Synthesis, molecular modeling, and biological evaluation of 4-[5-aryl-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazol-1-yl] benzenesulfonamides toward acetylcholinesterase, carbonic anhydrase I and II enzymes
Yamali, C (Yamali, Cem); Gul, HI (Gul, Halise Inci); Ece, A (Ece, Abdulilah); Taslimi, P (Taslimi, Parham); Gulcin, I (Gulcin, Ilhami)
CHEMICAL BIOLOGY & DRUG DESIGN 91:4,854-866 DOI: 10.1111/cbdd.13149, 2018
ÖzetIn this study, 4-[5-aryl-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazol-1-yl] benzenesulfonamides were synthesized, and inhibition effects on AChE, hCA I, and hCA II were evaluated. K-i values of the compounds toward hCA I were in the range of 24.2 +/- 4.6-49.8 +/- 12.8nm, while they were in the range of 37.3 +/- 9.0-65.3 +/- 16.7nm toward hCA II. K-i values of the acetazolamide were 282.1 +/- 19.7nm and 103.60 +/- 27.6nm toward both isoenzymes, respectively. The compounds inhibited AChE with K-i in the range of 22.7 +/- 10.3-109.1 +/- 27.0nm, whereas the tacrine had K-i value of 66.5 +/- 13.8nm. Electronic structure calculations at M06-L/6-31+G(d,p)//AM1 level and molecular docking studies were also performed to enlighten inhibition mechanism and to support experimental findings. Results obtained from calculations of molecular properties showed that the compounds obey drug-likeness properties. The experimental and computational findings obtained in this study might be useful in the design of novel inhibitors against hCA I, hCA II, and AChE.
Increased Potency and Selectivity for Group III Metabotropic Glutamate Receptor Agonists Binding at Dual sites
Selvam, C (Selvam, Chelliah); Lemasson, IA (Lemasson, Isabelle A.); Brabet, I (Brabet, Isabelle); Oueslati, N (Oueslati, Nadia); Karaman, B (Karaman, Berin); Cabaye, A (Cabaye, Alexandre); Tora, AS (Tora, Amelie S.); Commare, B (Commare, Bruno); Courtiol, T (Courtiol, Tiphanie); Cesarini, S (Cesarini, Sara); McCort-Tranchepain, I (McCort-Tranchepain, Isabelle); Rigault, D (Rigault, Delphine); Mony, L (Mony, Laetitia); Bessiron, T (Bessiron, Thomas); McLean, H (McLean, Heather); Leroux, FR (Leroux, Frederic R.); Colobert, F (Colobert, Francoise); Daniel, H (Daniel, Herve); Goupil-Lamy, A (Goupil-Lamy, Anne); Bertrand, HO (Bertrand, Hugues-Olivier); Goudet, C (Goudet, Cyril); Pin, JP (Pin, Jean-Philippe); Acher, FC (Acher, Francine C.)
JOURNAL OF MEDICINAL CHEMISTRY 61:5,1969-1989 DOI: 10.1021/acs.jmedchem.7b01438, 2018
ÖzetA group III metabotropic glutamate (mGlu) receptor agonist (PCEP) was identified by virtual HTS. This orthosteric ligand is composed by an L-AP4-derived fragment that mimics glutamate and a chain that binds into a neighboring pocket, offering possibilities to improve affinity and selectivity. Herein we describe a series of derivatives where the distal chain is replaced by an aromatic or heteroaromatic group. Potent agonists were identified, including some with a mGlu(4) subtype preference, e.g., 17m (LSP1-2111) and 16g (LSP4-2022). Molecular modeling suggests that aromatic functional groups may bind at either one of the two chloride regulatory sites. These agonists may thus be considered as particular bitopic/dualsteric ligands. 17m was shown to reduce GABAergic synaptic transmission at striatopallidal synapses. We now demonstrate its inhibitory effect at glutamatergic parallel fiber-Purkinje cell synapses in the cerebellar cortex. Although these ligands have physicochemical properties that are markedly different from typical CNS drugs, they hold significant therapeutic potential.
Design, Synthesis, SAR and Molecular Modeling Studies of Novel Imidazo[2,1-b][1,3,4]Thiadiazole Derivatives as Highly Potent Antimicrobial Agents
Tahtaci, H (Tahtaci, Hakan); Karacik, H (Karacik, Hatice); Ece, A (Ece, Abdulilah); Er, M (Er, Mustafa); Seker, MG (Seker, Mine Gul)
MOLECULAR INFORMATICS 37:3 DOI: 10.1002/minf.201700083, 2018
ÖzetIn this study, a novel series of phenyl substituted imidazo[2,1-b][1,3,4]thiadiazole derivatives were synthesized, characterized and explored for antibacterial activity against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Bacillus subtilis and antifungal activity against Candida albicans. Most of the synthesized compounds exhibited remarkable antimicrobial activities, some of which being ten times more potent than positive controls. The most promising compound showed excellent activity with MIC value of 0.03g/ml against both S. aureus and B. subtilis (MIC values of positive compound Chloramphenicol are 0.4g/ml and 0.85g/ml, respectively). Furthermore, structure-activity relationship was also investigated with the help of computational tools. Some physicochemical and ADME properties of the compounds were calculated too. The combination of electronic structure calculations performed at PM6 level and molecular docking simulations using Glide extra-precision mode showed that the hydrophobic nature of keto aryl ring with no electron withdrawing substituents at para position enhances activity while electron-donating substituents at the second aryl ring is detrimental to activity.
Vitamin D Deficiency in Pregnant Women and Their Infants
Ozdemir, AA (Ozdemir, Abdurrahman Avar); Gundemir, YE (Gundemir, Yasemin Ercan); Kucuk, M (Kucuk, Mustafa); Sarici, DY (Sarici, Deniz Yildiran); Elgormus, Y (Elgormus, Yusuf); Cag, Y (Cag, Yakup); Bilek, G (Bilek, Gunal)
JOURNAL OF CLINICAL RESEARCH IN PEDIATRIC ENDOCRINOLOGY 10:1,44-50 DOI: 10.4274/jcrpe.4706, 2018
ÖzetObjective: Vitamin D deficiency is a serious health problem despite a general improvement in socio-economic status in Turkey. The aim of this study was to evaluate maternal vitamin D status and its effect on neonatal vitamin D concentrations after a support programme for pregnant women was introduced. A second aim was to identify risk factors for vitamin D deficiency in a district of Istanbul.
Methods: A total of 97 pregnant women and 90 infants were included in this study, conducted between January and October 2016. The demographic data, risk factors and daily vitamin intake were recorded. Serum levels of vitamin D, calcium, phosphorus and alkaline phosphatase in all subjects were measured. The mothers and newborns were divided into groups based on their vitamin D levels. The relationship between vitamin D levels and risk factors was analyzed.
Results: Mean +/- standard deviation vitamin D levels for the women and their infants were found to be 14.82 +/- 11.45 and 13.16 +/- 7.16 ng/mL, respectively. The number of mothers and infants was significantly higher in the deficient group, and their mean vitamin D levels significantly lower (9.02 +/- 1.34 and 8.80 +/- 1.06 ng/mL, respectively) (p<0.001, p<0.001). Only 14.4% of pregnant women took 10001200 IU/day of vitamin D. When the mother groups were evaluated in terms of risk factors, there were significant differences in daily vitamin intake and clothing style (p<0.001 and p<0.001 respectively).
Conclusion: Vitamin D deficiency in pregnant women and their infants is still a serious health problem in Turkey, although a vitamin D support programme during pregnancy has been launched by the department of health.
Identification of Bichalcones as Sirtuin Inhibitors by Virtual Screening and In Vitro Testing
Karaman, B (Karaman, Berin); Alhalabi, Z (Alhalabi, Zayan); Swyter, S (Swyter, Soeren); Mihigo, SO (Mihigo, Shetonde O.); Andrae-Marobela, K (Andrae-Marobela, Kerstin); Jung, M (Jung, Manfred); Sippl, W (Sippl, Wolfgang); Ntie-Kang, F (Ntie-Kang, Fidele)
MOLECULES 23:2 DOI: 10.3390/molecules23020416, 2018
ÖzetSirtuins are nicotinamide adenine dinucleotide (NAD(+))-dependent class III histone deacetylases, which have been linked to the pathogenesis of numerous diseases, including HIV, metabolic disorders, neurodegeneration and cancer. Docking of the virtual pan-African natural products library (p-ANAPL), followed by in vitro testing, resulted in the identification of two inhibitors of sirtuin 1, 2 and 3 (sirt1-3). Two bichalcones, known as rhuschalcone IV (8) and an analogue of rhuschalcone I (9), previously isolated from the medicinal plant Rhus pyroides, were shown to be active in the in vitro assay. The rhuschalcone I analogue (9) showed the best activity against sirt1, with an IC50 value of 40.8 mu M. Based on the docking experiments, suggestions for improving the biological activities of the newly identified hit compounds have been provided.
Respiratory muscle strength in patients with pulmonary hypertension: The relationship with exercise capacity, physical activity level, and quality of life
Aslan, GK (Aslan, Goksen Kuran); Akinci, B (Akinci, Buket); Yeldan, I (Yeldan, Ipek); Okumus, G (Okumus, Gulfer)
CLINICAL RESPIRATORY JOURNAL 22:2, 699-705 DOI: 10.1111/crj.12582, 2018
ÖzetIntroductionPulmonary hypertension (PH) is characterized by exertional dyspnea, fatigue, chest pain, dizziness, and syncope. Physical activity, peripheral, and respiratory muscle strength reduces in pateints with PH. Little is known about respiratory muscle weakness and related outcomes.
ObjectivesThe aims of the study were to determine respiratory muscle strength and to investigate the relationship between respiratory muscle strength and spirometric measurements, exercise capacity, physical activity level, quality of life, and pulmonary hemodynamics in patients with PH.
MethodsIn total, 33 patients aged 25-80 years who were diagnosed as having PH and 24 healthy volunteers were included in the study. To measure respiratory function, spirometry, maximal inspiratory (MIP), and expiratory pressures (MEP) were used. Physical activity level was determined with activity monitoring (SenseWear Armband) and the International Physical Activity Questionnaire-Short Form. Exercise capacity was determined using the 6-minute walk test. Quality of life was evaluated with the Minnesota Living with Heart Failure Questionnaire (MLHFQ).
ResultsMaximal inspiratory pressure and MEP values of the patients with PH were significantly lower than the age- and sex-matched healthy controls (P<.0001). Significant relationships were found between the MIP and six MWD (r=.40, P=.02), vigorous physical activity (r=.38, P=.03), moderate physical activity (r=61, P<.001), and arm band-average metabolic equivalent (r=.39, P=.02).
ConclusionThe relationship between maximum inspiratory pressure, exercise capacity, and physical activity level showed that a decrease in exercise capacity or physical activity level may be a predictor for decreased MIP.
Experimental and theoretical studies on tautomeric structures of a newly synthesized 2,2'(hydrazine-1,2-diylidenebis(propan-1-yl-1-ylidene)) diphenol
Karakurt, T (Karakurt, Tuncay); Cukurovali, A (Cukurovali, Alaaddin); Subasi, NT (Subasi, Nuriye Tuna); Onaran, A (Onaran, Abdurrahman); Ece, A (Ece, Abdulilah); Eker, S (Eker, Sitki); Kani, I (Kani, Ibrahim)
CHEMICAL PHYSICS LETTERS 693,132-145 DOI: 10.1016/j.cplett.2018.01.016,2018
ÖzetIn the present study, a single crystal of a Schiff base, 2,2'(hydrazine-1,2-diylidenebis(propan-1-yl-1-ylidene)) diphenol, was synthesized. The structure of the synthesized crystal was confirmed by H-1 and C-13 NMR spectroscopic and X-ray diffraction analysis techniques. Experimental and theoretical studies were carried out on two tautomeric structures. It has been observed that the title compound studied can be in two different tautomeric forms, phenol-imine and keto-amine. Theoretical calculations have been performed to support experimental results. Accordingly, the geometric parameters of the compound were optimized by the density functional theory (DFT) method using the Gaussian 09 and Quantum Espresso (QE) packet program was used for periodic boundary conditions (PBC) studies. Furthermore, the compound was also tested for in vitro antifungal activity against Sclerotinia sclerotiorum, Alternaria solani, Fusarium oxysporum f. sp. lycopersici and Monilinia fructigena plant pathogens. Promising inhibition profiles were observed especially towards A. solani. Finally, molecular docking studies and post-docking procedure based on Molecular Mechanics-Generalized Born Surface Area (MM-GBSA) were also carried out to get insight into the compound's binding interactions with the potential. Although theoretical calculations showed that the phenol-imine form was more stable, keto-amine form was predicted to have better binding affinity which was concluded to result from loss of rotational entropy in phenol-imine upon binding. The results obtained here from both experimental and computational methods might serve as a potential lead in the development of novel anti-fungal agents. (C) 2018 Elsevier B.V. All rights reserved.
Inhibition of mTOR pathway decreases the expression of pre-meiotic and meiotic markers throughout postnatal development and in adult testes in mice
Sahin, P (Sahin, P.); Gungor-Ordueri, NE (Gungor-Ordueri, N. E.); Celik-Ozenci, C (Celik-Ozenci, C.)
ANDROLOGIA 50:1 DOI: 10.1111/and.12811,2018
ÖzetRapamycin (mTOR inhibitor) has been reported to have negative effect on human male gonadal function. Previously, we showed that mTOR signalling molecules are expressed during early spermatogenesis in mice. The objective of this study was to investigate the role of mTOR signalling in meiosis both during the first wave of spermatogenesis and also during adult spermatogenesis. Day 5 post-partum mice were administered rapamycin and retinoic acid (RA; a Stra8 activator), and expression of p-p70S6K and Stra8 proteins was evaluated. p-p70S6K and Stra8 protein expressions decreased in post-natal testes after rapamycin treatment. Stra8 protein expression increased after RA and rapamycin+RA administrations in post-natal testes. In adult mice, rapamycin was administrated for 1 or 4weeks. Morphological analysis for testicular damage and TUNEL assay was performed. After rapamycin administration, germ cell loss increased in adult testes. Ultrastructural analysis revealed disorganised testicular morphology and vacuolisation. The number of apoptotic germ cells increased after 4weeks rapamycin administration. Stra8 and Dmc1 expressions decreased in 4weeks rapamycin group, whereas Sycp3 and VASA expression did not change. Our findings suggest that mTOR pathway has an important role in meiotic progress of male germ cells both during first wave of spermatogenesis and in adult mice.
The Comparison of Angiographic Scoring Systems With the Predictors of Atherosclerosis
Kalkan, K (Kalkan, Kamuran); Hamur, H (Hamur, Hikmet); Yildirim, E (Yildirim, Erkan); Ipek, E (Ipek, Emrah); Ermis, E (Ermis, Emrah); Ozturk, M (Ozturk, Mustafa); Karal, H (Karal, Huseyin); Korkmaz, AF (Korkmaz, Ali Fuat); Bayantemur, M (Bayantemur, Murat); Demirelli, S (Demirelli, Selami)
ANGIOLOGY 69:2,158-163 DOI: 10.1177/0003319717712118, 2018
ÖzetSyntax score (SS) and Gensini score (GS) are used to determine the complexity and severity of coronary artery disease. Although there are some studies indicating the individual relationship of these scoring systems with the carotid intima-media thickness (cIMT) and epicardial fat tissue (EFT), there was no previous study that compared the SS and GS in that respect. We aimed to assess the relationship of SS and GS with predictors of atherosclerosis. We enrolled 522 patients. There were positive correlations between GS and SS tertiles with cIMT and EFT. According to multivariate linear regression analysis for GS, EFT (: 0.035, t: 2.63, and P = .49) and cIMT (: 0.339, t: 2.97, and P = .053) were not independently associated. For SS, EFT (: 0.009, t: 6.5, and P = .006) and cIMT (: 1.2, t: 10.1, and P = .001) were independently and significantly associated. We showed that the SS is significantly associated more with surrogate markers of atherosclerosis such as EFT and cIMT than the GS.
Can Follicular Fluid Anti-Mullerian Hormone Level Be a Determinant of Pregnancy in Women Under 35 Years of Age?
Yilmaz, T (Yilmaz, Turabi); Tavukcuoglu, S (Tavukcuoglu, Safak); Tasdemir, S (Tasdemir, Seval); Esenkaya, S (Esenkaya, Semra); Irez, T (Irez, Tulay)
INTERNATIONAL JOURNAL OF WOMENS HEALTH AND REPRODUCTION SCIENCES 6:1,6-10 DOI: 10.15296/ijwhr.2018.03, 2018
ÖzetObjectives: The failure of oocyte morphological assessment to predict in vitro fertilization (IVF) outcome has led the researchers to examine biochemical criteria of follicular fluid (FF). Anti-Mullerian hormone (AMH), an important marker of ovarian reserve, is secreted by the granulosa cells. The aim of this study is to investigate the relationship between the FF (AMH) levels and oocyte, embryo quality, fertilization, and clinical pregnancy.
Materials and Methods: The FF (AMH) levels of 61 patients (mean age: 33.72 +/- 4.82 years; range: 21 to 42 years) were analyzed. The FF (AMH) levels were measured by the quantitative auto-analyzer with an electro-chemiluminescence assay.
Results: The FF (AMH) levels in patients under 35 years of age were higher in pregnant women than non-pregnant ones (P<0.01).
Conclusion: Our study results showed that the FF (AMH) levels were correlated with the quality of oocyte and embryo and were the predictors of clinical pregnancy in patients younger than 35 years of age.
Determining the relationship between body mass index, healthy lifestyle behaviors and social appearance anxiety
Sanlier, N (Sanlier, Nevin); Pehlivan, M (Pehlivan, Merve); Sabuncular, G (Sabuncular, Guleren); Bakan, S (Bakan, Sevinc); Isguzar, Y (Isguzar, Yesim)
ECOLOGY OF FOOD AND NUTRITION 57:2,124-139 DOI: 10.1080/03670244.2017.1419343,2018
ÖzetThe aim of this study was to describe the relationship between nutritional status, healthy lifestyle behaviors and social appearance anxiety of individuals aged 20-65 (n=500). A significant, positive relationship was seen between body mass index and nutrition, and negative relationship between body mass index and spiritual development, interpersonal relations, stress management. There was a negative relationship between the ORTO-15 score and health responsibility, physical activity, nutrition, spiritual development, interpersonal relationships, stress management. There was also a negative relationship between the Social Appearance Anxiety Scale and spiritual development, interpersonal relationships.
Bonding Strength of Universal Adhesives To Er,Cr:YSGG Laser-Irradiated Dentin
Ayar, MK (Ayar, M. K.); Erdermir, F (Erdermir, F.)
NIGERIAN JOURNAL OF CLINICAL PRACTICE 21:1,93-98 DOI: 10.4103/1119-3077.224792, 2018
ÖzetObjectives: Universal adhesives have been recently introduced for use as self-etch or etch-and-rinse adhesives depending on the dental substrate and clinical condition. However, their bonding effectiveness to laser-irradiated dentin is still not well known. Therefore, the aim of this study was to compare the shear bond strength (SBS) of two universal adhesives (Single Bond Universal, Nova Compo-B Plus) applied following laser-etching with SBS of the same adhesives applied in self-etch and acid-etch modes, respectively. Materials and Methods: Sixty bovine incisors were used to obtain the flattened dentin surfaces. Specimens were divided into two groups according to universal adhesives. Each universal adhesive was applied with one of the following modes, self-etch, acid-etch, or laser-etch (n = 10). Er,Cr:YSGG laser was used for laser-etching with 1.5 Wu20 Hz parameters. After adhesive applications and composite buildups, SBS was determined after storage in water for 24 h using a universal testing machine with a crosshead speed of 0.5 mm/min. Failure modes were evaluated using a stereomicroscope. Data were analyzed using two-way of analyses of variances (ANOVA) (P = 0.05). Results: Two-way ANOVA revealed that adhesive had no effect on SBS (P > 0.05), but application mode significantly influenced SBS (P < 0.001). Laser-etch significantly increased SBS for NCP when compared to self-etch mode, whereas laser-etch provided similar SBS with self-etch mode for SBU. Conclusions: The influence of different application modes on dentin bond strength of universal adhesives was dependent on the adhesive material. Clinical Significance: For universal adhesives, laser etching may provide some benefits on bonds strength but this would depend on product.
Birth of Industry 5.0: Making Sense of Big Data with Artificial Intelligence, "The Internet of Things" and Next-Generation Technology Policy
Ozdemir, V (Ozdemir, Vural); Hekim, N (Hekim, Nezih)
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY 22:1,65-76 DOI: 10.1089/omi.2017.0194, 2018
ÖzetDriverless cars with artificial intelligence (AI) and automated supermarkets run by collaborative robots (cobots) working without human supervision have sparked off new debates: what will be the impacts of extreme automation, turbocharged by the Internet of Things (IoT), AI, and the Industry 4.0, on Big Data and omics implementation science? The IoT builds on (1) broadband wireless internet connectivity, (2) miniaturized sensors embedded in animate and inanimate objects ranging from the house cat to the milk carton in your smart fridge, and (3) AI and cobots making sense of Big Data collected by sensors. Industry 4.0 is a high-tech strategy for manufacturing automation that employs the IoT, thus creating the Smart Factory. Extreme automation until "everything is connected to everything else" poses, however, vulnerabilities that have been little considered to date. First, highly integrated systems are vulnerable to systemic risks such as total network collapse in the event of failure of one of its parts, for example, by hacking or Internet viruses that can fully invade integrated systems. Second, extreme connectivity creates new social and political power structures. If left unchecked, they might lead to authoritarian governance by one person in total control of network power, directly or through her/his connected surrogates. We propose in this study, Industry 5.0 that can democratize knowledge coproduction from Big Data, building on the new concept of symmetrical innovation. Industry 5.0 utilizes IoT, but differs from predecessor automation systems by having three-dimensional (3D) symmetry in innovation ecosystem design: (1) a built-in safe exit strategy in case of demise of hyperconnected entrenched digital knowledge networks. Importantly, such safe exists are orthogonalin that they allow "digital detox" by employing pathways unrelated/unaffected by automated networks, for example, electronic patient records versus material/article trails on vital medical information; (2) equal emphasis on both acceleration and deceleration of innovation if diminishing returns become apparent; and (3) next generation social science and humanities (SSH) research for global governance of emerging technologies: "Post-ELSI Technology Evaluation Research" (PETER). Importantly, PETER considers the technology opportunity costs, ethics, ethics-of-ethics, framings (epistemology), independence, and reflexivity of SSH research in technology policymaking. Industry 5.0 is poised to harness extreme automation and Big Data with safety, innovative technology policy, and responsible implementation science, enabled by 3D symmetry in innovation ecosystem design.
Evaluation of disordered eating tendencies in young adults
Sanlier, N (Sanlier, Nevin); Varli, SN (Varli, Semra Navruz); Macit, MS (Macit, M. Sedanur); Mortas, H (Mortas, Hande); Tatar, T (Tatar, Tugba)
EATING AND WEIGHT DISORDERS-STUDIES ON ANOREXIA BULIMIA AND OBESITY 22:4,623-631 DOI: 10.1007/s40519-017-0430-9, 2017
ÖzetPurpose It was aimed to determine the prevalence of high disordered eating tendencies and its relationship with food addiction, emotional eating and self esteem in participants at 18 and 33 years age group.
Methods This study was planned as a cross-sectional study and conducted with 1359 young adult volunteers (M = 386, F = 973) with an average age of 22.4 +/- 2.84 years. Eating Attitudes Test-26 (EAT-26), Yale Food Addiction Scale (YFAS), Emotional Appetite Questionnaire (EMAQ) and Rosenberg Self-Esteem Scale (RSES) were used. EAT-26 score above 20 was considered as eating disorders risk cutoff.
Results Participants with disordered eating tendencies have higher rates (22.4%) of food addiction compared to participants without high disordered eating tendencies (7.2%). There is no difference for EMAQ and YFAS scores; however, there is a significant difference for RSES and EAT-26 scores according to gender. A positive association of EAT-26 with YFAS and EMAQ-negative scores and a negative association of EAT-26 with RSES and EMAQ-positive were found.
Discussion There is association among EAT-26, YFAS, and Rosenberg Self-Esteem Scale, and Emotional Appetite Questionnaire scores. This study provides information for future studies about high disordered eating tendencies, food addiction and mood that are thought to be important in young adults.
Omega-3 polyunsaturated fatty acids reduce vascular tone and inflammation in human saphenous vein
Daci, A (Daci, Armond); Ozen, G (Ozen, Giilsev); Uyar, I (Uyar, Imran); Civelek, E (Civelek, Erkan); Yildirim, FIA (Yildirim, F. Ilkay Alp); Durman, DK (Durman, Deniz Kaleli); Teskin, O (Teskin, Onder); Norel, X (Norel, Xavier); Uydes-Dogan, BS (Uydes-Dogan, B. Sonmez); Topal, G (Topal, Gokce)
PROSTAGLANDINS & OTHER LIPID MEDIATORS 133:SI, DOI: 10.10164/j.prostaglandins.2017.08.007,2017
ÖzetDietary intake of omega-3 polyunsaturated fatty acids, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), has been reported to have beneficial cardiovascular effects. However, little is known about the effect of EPA and DHA on human vascular tone. Therefore, the aim of this study is to evaluate the effect of EPA and DHA on vascular tone of the human saphenous vein (SV) obtained from patients undergoing coronary bypass operation under normal and inflammatory conditions. Moreover, we aimed to investigate the effect of EPA and DHA on the release of inflammatory mediators from SV. Pretreatment of SV with EPA and DHA (100 mu M, 18 h) decreased the contractile response of SV to norepinephrine (NE) under normal and inflammatory conditions. Moreover, EPA and DHA pretreatment diminished increased Monocyte Chemoattractant Protein-1 (MCP-1) and Tumor Necrosis Factor-alpha (TNF-alpha) release from SV under inflammatory conditions. In conclusion, our results suggest that EPA and DHA pretreatment may be beneficial to counteract graft vasospasm and vascular inflammation in SV which are important factors in graft failure development. Therefore, dietary intake of EPA and DHA may have potential clinical applications in improving coronary bypass graft patency.
Relaxant effect of the prostacyclin analogue iloprost on isolated human radial artery: An approach for the reversal of graft spasm
Engin, E (Engin, Ersoy); Yildirim, FIA (Yildirim, F. Ilkay Alp); Durman, DK (Durman, Deniz Kaleli); Omeroglu, SN (Omeroglu, Suat Nail); Goksedef, D (Goksedef, Deniz); Teskin, O (Teskin, Onder); Balkanay, OO (Balkanay, Ozan Onur); Ipek, G (Ipek, Gokhan); Dogan, BSU (Dogan, B. Sonmez Uyde)
PROSTAGLANDINS & OTHER LIPID MEDIATORS 133:SI,35-41 DOI: 10.1016/j.prostaglandins.2017.10.003, 2017
ÖzetRadial artery graft spasm in the perioperative or postoperative period of coronary bypass surgery necessitates urgent treatment due to risk of graft failure and mortality. Herein, we evaluated the effect of iloprost, a prostacyclin (PGI(2)) analogue, against the contractions produced by noradrenaline and potassium chloride on isolated human radial artery. Following the determination of endothelial and vascular relaxing capacities of the arteries, iloprost (10(-9)M-10(-6)M) was cumulatively applied on rings precontracted submaximally with the spasmogens. In some rings, the response to iloprost was assessed following pretreatment with nitric oxide (NO) synthase inhibitor, L-NAME (3 x 10(-4)M,30 min). Iloprost produced complete relaxations on radial artery rings pre contracted with noradrenaline whereas, only moderate relaxations against the contractions induced by potassium chloride. Notably, the relaxation to iloprost was remarkably blunted in radial arteries with impaired endothelial function. Moreover, the relaxation to iloprost was unchanged in rings pretreated with L-NAME. Our results demonstrated that iloprost could be a potent relaxant agent in reversing radial artery spasm, particularly initiated by noradrenaline, possibly acting via an endothelium-mediated mechanism unrelated to NO.
Stop the Spam! Conference Ethics and Decoding the Subtext in Post-Truth Science. What Would Denis Diderot Say?
Ozdemir, V (Ozdemir, Vural); Dandara, C (Dandara, Collet); Hekim, N (Hekim, Nezih); Birch, K (Birch, Kean); Springer, S (Springer, Simon); Kunej, T (Kunej, Tanja); Endrenyi, L (Endrenyi, Laszlo)
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY 21:11,658-664 DOI: 10.1089/omi.2017.0150, 2017
ÖzetScience and its practice always had a subtext, subject to influence by scientists', funders', and other innovation actors' values and assumptions. The recent emergence of post-truth, authoritarian and populist penchants, in both developed and developing countries, has further blurred the already fluid boundaries between material scientific facts and their social construction/shaping by scientific subtext, human values, powers, and hegemony. While there are certain checks, balances, and oversight mechanisms for publication ethics, other pillars of science communication, most notably, scientific conferences and their governance, are ill prepared for post-truth science. Worrisomely, the proliferation of spam conferences is a major cause for concern for integrative biology and postgenomic science. The current gaps in conference ethics are important beyond science communication because conferences help build legitimacy of emerging technologies and frontiers of science and, thus, bestows upon the organizers, funders, enlisted scientific advisors, speakers, among others, power, which in turn needs to be checked. Denis Diderot (1713-1784), a prominent intellectual during the Enlightenment period, has aptly observed that the very act of organizing brings about power, influence, and control. If the subtext of conference practices is left unchecked, it can pave the way for hegemony, and yet more volatile and violent authoritarian governance systems in science and society. This begs for innovative solutions to increase accountability, resilience, and capacity of technology experts and scientists to discern and decode the subtext in science and its communication in the current post-truth world. We propose that the existing undergraduate and graduate programs in life and physical sciences and medicine could be redesigned to include a rotation for exposure to and training in political science. Such innovative PhD+ programs straddling technical and political science scholarship would best equip future students and citizens to grasp and respond to subtext and embedded opaque value and power systems in scientific practices in an increasingly post-truth world. Political science scholarship unpacks the inner workings, subtext, and power dynamics in science and society. Thus, knowledge of political science competency is akin to molecular biology in life sciences. Both make the invisible (e.g., cell biology versus subtext of knowledge) visible. The ability to read subtext in science and claims of post-truth knowledge is a new and essential form of societal literacy in 21st century science and integrative biology.
Enuresis in Early Adolescents
Savaser, S (Savaser, Sevim); Kizilkaya Beji, N (Kizilkaya Beji, Nezihe); Aslan, E (Aslan, Ergul); Gozen, D (Gozen, Duygu)
IRANIAN JOURNAL OF PUBLIC HEALTH 46:10,1452-1453 OCT 2017
Surfactant proteins in psoriasis vulgaris and lichen planus
Akman-Karakas, A (Akman-Karakas, A.); Celik-Ozenci, C (Celik-Ozenci, C.); Kipmen-Korgum, D (Kipmen-Korgum, D.); Gungor-Ordueri, NE (Gungor-Ordueri, N. E.); Cift-Cioglu, MA (Cift-cioglu, M. A.); Alpsoy, E (Alpsoy, E.)
JOURNAL OF INVESTIGATIVE DERMATOLOGY 137:10,S276-S276, DOI: 10.1016/j.jid.2017.07.690,2017
Effect of fasting on 24-h blood pressure values of individuals with no previous history of hypertension
Seker, A (Seker, Ayse); Demirci, H (Demirci, Hakan); Ocakoglu, G (Ocakoglu, Gokhan); Aydin, U (Aydin, Ufuk); Ucar, H (Ucar, Hakan); Yildiz, G (Yildiz, Gursel); Yaman, O (Yaman, Ozen)
BLOOD PRESSURE MONITORING 22:5, 247-252,, DOI: 10.1097/MBP.0000000000000244 , 2017
Özet için tıklayınızObjective We aimed to analyze the difference in 24-h blood pressure values during Ramadan of fasting and nonfasting individuals with no previous history of hypertension.
Patients and methods This study was planned as a multicenter research study in the cities of Izmit, Zonguldak, Sivas, and Adana. The percentage changes in the blood pressure of the patients were calculated from their blood pressure measurements at 0 h while they were resting. A Food Frequency Questionnaire was filled out by all participants.Results Forty patients were included in the fasting group and 55 patients were included in the nonfasting group in the study. There was a difference between the two groups in percent changes of systolic measurements performed at the 4th (5% difference and P=0.020), 13th (6% difference and P=0.015), 14th (10% difference and P=0.017), 18th (9% difference and P=0.027), 19th (9% difference and P=0.020), and 20th (6% difference and P=0.014) hours with respect to the baseline measurement at the 0 h. There was a difference between the fasting and nonfasting groups in the Fasting Food Questionnaire results. Meat consumption was significantly higher in the fasting group.
Conclusion A systolic blood pressure increase in fasting patients was observed in measurements at 18:00, 19:00, and 20:00 h. We believe that an increase of more than 10% in blood pressure at the time of iftar is an important result of our study in terms of the meal preferences of the individuals under risk. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.
The Comparison of The Effects of Application of Compound 48/80 and Autologous Mast Cell Mediator Suspension on Plasma Substance-P Levels And Dural Mast Cells in Rats: A Methodological Study For Acute Inflammatory Pain
Kilinc, E (Kilinc, Erkan); Dagistan, Y (Dagistan, Yasar); Cetinkaya, A (Cetinkaya, Ayhan); Tore, F (Tore, Fatma)
ACTA PHYSIOLOGICA 221:SI,34-34 SEP 2017
The Neurotrophic Effect of Thymoquinone in Pentilentetrazole-induced Epilepsy Model
Pala, M (Pala, Mukaddes); Acikgoz, NP (Acikgoz, Nilgun Pala); Kacmaz, SK (Kacmaz, Sema Karaca); Acar, S (Acar, Seyma); Meral, I (Meral, Ismail); Akbas, F (Akbas, Fahri)
ACTA PHYSIOLOGICA 221:SI, 37-38 SEP 2017
Salmon Calcitonin Ameliorates Nitroglycerin-Induced Migraine Pain Through Inhibition of Calcitonin-Gene Related Peptide Release From Trigeminal Neurons and C-Fos Expression in Trigeminal Nucleus Caudalis in Rats
Kilinc, E (Kilinc, Erkan); Dagistan, Y (Dagistan, Yasar); Kukner, A (Kukner, Aysel); Soyler, G (Soyler, Gizem); Tore, F (Tore, Fatma)
ACTA PHYSIOLOGICA 221:SI,72-72 SEP 2017
Investigation of the Effects of Seizures Induced with Penthylenetrazole on Biomolecular Structure in Peripheral Tissues with Fourier Transform Infrared Spectroscopy Method and Comparison with Oxidative Stress Markers
Uzum, G (Uzum, Gulay); Kuruca, S (Kuruca, Serap); Kaptan, Z (Kaptan, Zulal); Kapucu, A (Kapucu, Aysegul); Dar, KA (Dar, Kadriye Akgun); Yilmaz, A (Yilmaz, Ayberk); Yalcinkaya, OB (Yalcinkaya, Olcay Bolukbasi); Kartal, S (Kartal, Sehban); Kaleler, I (Kaleler, Islim)
ACTA PHYSIOLOGICA 221:SI,73-74 SEP 2017
Evaluation of Daily Blood Pressure Alteration in Subclinical Hypothyroidism
Canbolat, IP (Canbolat, Ismail Polat); Belen, E (Belen, Erdal); Bayyigit, A (Bayyigit, Akif); Helvact, A (Helvact, Aysen); Kilickesmez, K (Kilickesmez, Kadriye)
ACTA CARDIOLOGICA SINICA, 33:5, 489-494, DOI: 10.6515/ACS20170220B, 2017
Özet için tıklayınızBackground: Subclinical hypothyroidism is the most common thyroid dysfunction in the general population. The relationship between overt thyroid dysfunction and hypertension is generally understood. Besides high blood pressure, non-dipper hypertension is known to increase cardiovascular risk. Our aim is to investigate daily blood pressure changes and the frequency of non-dipping patterns in patients with subclinical hypothyroidism.
Methods: Forty-nine patients without hypertension with subclinical hypothyroidism were compared with 50 healthy sex-and age-matched controls using ambulatory blood pressure monitoring.
Results: Thyroid-stimulating hormone (TSH) levels were significantly higher in the subclinic hypothyroidism group, and there was no difference between free triiodothyronine (FT3) and free thyroxine (FT4) levels which could be predicted as a result of the study design. Levels of mean diastolic, daytime diastolic, nighttime diastolic and nighttime systolic blood pressure were significantly higher in the subclinic hypothyroidism group (p = 0.001 for mean, daytime and nighttime diastolic and p = 0.01 for nighttime systolic). Diastolic non-dipping occurred more frequently in the subclinic hypothyroidism group [subclinical hypothyroidism group 24 patients (49%), control group 13 patients (26%), p = 0.01]. On multivariate analysis, subclinical hypothyroidism was independently associated with diastolic non-dipping (95% confidence interval 1.162-8.053, odds ratio 1.182, p = 0.024).
Conclusions: Our study found that both the frequency of diastolic non-dipping pattern and diastolic blood pressure increase with subclinical hypothyroidism. Therfore, it would appear that searching for non-dipping pattern can add valuable information for patients with subclinical hypothyroidism.
Coping and Help-Seeking Behaviors for Management of Urinary Incontinence
Bilgic, D (Bilgic, Dilek); Kizilkaya Beji, N (Kizilkaya Beji, Nezihe); Ozbas, A (Ozbas, Ayfer); Cavdar, I (Cavdar, Ikbal); Aslan, E (Aslan, Ergul); Yalcin, O (Yalcin, Onay)
LUTS-LOWER URINARY TRACT SYMPTOMS, 9:3, 134-141, DOI: 10.1111/luts.12120, 2017
Özet için tıklayınızObjectivesTo describe the coping strategies and help-seeking behaviors of women and men with urinary incontinence (UI).
MethodsIn cross-sectional study, 156 women and 106 men Turkish with UI were interviewed using a questionnaire covering 29 questions.
ResultsIt was determined that 50.6% of women and 34.0% of men seek help in first for treatment of UI. Forty eight percent of men consulted a physician in the first 6 months after the UI occurred, 44.9% of women consulted a physician 2-5 years later after the UI occurred. Coping behaviors of both groups for management of the UI were as follows: keeping feet warm, performing hot application to perineum, reducing the amount of daily drinking water, using pad, cloth pads, restricting physical activity, refraining from social life and praying etc.
ConclusionsWhile women mostly do not prefer to seek medical advice for UI, men tend to go to doctors more often than women. Except for treatment seeking behaviors, both men and women are practicing coping methods for the management of UI such as going frequently to the toilet, keeping feet warm, hot application to perineum etc.
Familial early infantile epileptic encephalopathy and cardiac conduction disorder: A rare cause of SUDEP in infancy
Turkdogan, D (Turkdogan, Dilsad); Usluer, S (Usluer, Sunay); Akalin, F (Akalin, Figen); Agyuz, U (Agyuz, Umut); Aslan, ES (Aslan, Elif Sibel)
SEIZURE-EUROPEAN JOURNAL OF EPILEPSY, 50, 171-172, DOI: 10.1016/j.seizure.2017.06.019, 2017
Gustilo type IIIC open tibia fractures with vascular repair: minimum 2-year follow-up
Tunali, O (Tunali, O.); Saglam, Y (Saglam, Y.); Balci, HI (Balci, H. I.); Kochai, A (Kochai, A.); Sahbaz, NA (Sahbaz, N. A.); Sayin, OA (Sayin, O. A.); Yazicioglu, O (Yazicioglu, O.)
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY, 43:4, 505-512, DOI: 10.1007/s00068-016-0689-y, 2017
Özet için tıklayınızPurpose Salvage or amputation for grade 3C open fracture of tibia is not well responded question universally because of surgical innovations, cultural believes, difficulties in estimate the outcome, coasts, and different results in the literature. The aim of this study was to evaluate the surgical outcomes of Gustilo grade 3C open tibia fractures with at least two years follow-up in non-military adults.
Methods Twenty-two non-military patients with a mean age of 31.1 were operated with grade 3c open fractures at tibia level in last 10 years in our clinic. We evaluated them retrospectively and asked about their daily life, pain, and if present, about the wish for secondary amputation. We also asked if they would prefer a first day amputation rather than their present status.
Results Mean operation time after the injury was 13 h. Seven patients had nerve injury. Mean operation number was 3.5. Eight patients (%36) (all due to circulatory problem) had to have amputation. All patients treated with temporary unilateral external fixation than converted to circular external fixators when soft tSayı healing was completed. Two patients were reoperated because of deformity. Four patients needed revision surgery because of non-union. At long term follow, we had osteomyelitis in one patient.
Conclusions Scoring systems and the ischemic time are not the only predictors of amputation. The decision of the treatment mode should be made by the patient and the care team after discussing the options and outcomes rather than relying on a scoring system.
Assessment of the association between the presence of fragmented QRS and the predicted risk score of sudden cardiac death at 5 years in patients with hypertrophic cardiomyopathy
Ozyilmaz, S (Ozyilmaz, Sinem); Akgul, O (Akgul, Ozgur); Uyarel, H (Uyarel, Huseyin); Pusuroglu, H (Pusuroglu, Hamdi); Karayakali, M (Karayakali, Muammer); Gul, M (Gul, Mehmet); Cetin, M (Cetin, Mustafa); Satilmisoglu, H (Satilmisoglu, Hulusi); Yildirim, A (Yildirim, Aydin); Bakir, I (Bakir, Ihsan)
ANATOLIAN JOURNAL OF CARDIOLOGY, 18:1, 54-61, DOI: 10.14744/AnatolJCardiol.2017.7593 Basım Tarihi: JUL 2017
Özet için tıklayınızObjective: It has been shown that the presence of fragmented QRS (fQRS) is associated with poor prognosis in many cardiovascular diseases and in patients with hypertrophic cardiomyopathy (HCM). However, no study has shown an association with the absolute risk score of sudden cardiac death. The aim of this study was to determine the relationship between QRS and the predicted risk score of sudden cardiac death at 5 years (HCM Risk-SCD) in HCM patients.
Methods: In total, 115 consecutive HCM patients were included in this prospective observational study. The patients were divided into two groups according to the presence [fQRS(+) group (n= 65)] or absence [fQRS(-) group (n= 50)] of fQRS on a 12-lead electrocardiogram (ECG). Results: The HCM Risk-SCD (%) HCM Risk-SCD (>6%) values and some echocardiographic parameters, including ventricular extrasystole, ventricular tachycardia, cardiopulmonary resuscitation, implantable cardioverter defibrillator implantation, appropriate shock, and heart failure at the time of admission, were significantly higher in the fQRS(+) group than in the fQRS(-) group (all p<0.05). Both univariate and multivariate analyses revealed fQRS and New York Heart Association (NYHA) class as independent predictors of HCM Risk-SCD. In a receiver operating characteristic (ROC) curve analysis, an HCM Risk-SCD value of >4 was identified as an effective cut-off point in fQRS for HCM. An HCM Risk-SCD value of >4 yielded a sensitivity of 77% and a specificity of 76%.
Conclusion: fQRS is determined to be an independent high-risk indicator of HCM Risk-SCD. It seems to be associated with increased ventricular arrhythmias and some echocardiographic parameters.
The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores
Ipek, E (Ipek, Emrah); Ermis, E (Ermis, Emrah); Uysal, H (Uysal, Handan); Kizilet, H (Kizilet, Halit); Demirelli, S (Demirelli, Selami); Yildirim, E (Yildirim, Erkan); Unver, S (Unver, Sedat); Demir, B (Demir, Bircan); Kizilet, N (Kizilet, Nergiz)
ANATOLIAN JOURNAL OF CARDIOLOGY, 17:6, 483-489, DOI: 10.14744/AnatolJCardiol.2017.7582, 2017
Özet için tıklayınızObjective: We aimed to evaluate the relationship of micronucleus (MN) frequency and nuclear division index (NDI) with SYNTAX and Gensini scores and thrombolysis in myocardial infarction (TIMI) frame counts of coronary arteries in patients undergoing coronary angiography.
Methods: In a single-center prospective observational study, a total of 63 individuals, 48 consecutive patients with coronary artery disease (CAD) and 15 healthy people were included. Before coronary angiography (exposure to X-ray), blood samples were collected for lymphocyte cultures, MN and NDI measurements. According to the SYNTAX and Gensini scores, patients were allocated into two groups. Group 1 and 2 included the patients with SYNTAX scores <22 and >= 22 points, respectively. Similarly, groups according to Gensini scores included the ones <23 and >= 23 points. MN test was used for in vitro studies in human peripheral lymphocytes. Binucleated lymphocytes were calculated for each patient.
Results: MN frequency was significantly higher in group 2 than group 1 and in group 1 than control group (p<0.001). NDI was significantly higher in control group than group 1 and in group 1 than group 2 (p=0.003). MN frequency had positive but moderate correlation with SYNTAX and Gensini scores and TFCs of left anterior descending (LAD), circumflex and right coronary arteries (r=0.394, p=0.003; r=0.458, p<0.001; r=0.425, p<0.001; r=0.469, p<0.001; and r=0.475, p<0.001, respectively).
Conclusion: We can conclude that as the degree of atherosclerosis increases and coronary flow worsens, MN frequency increases and NDI decreases. Our results may help to elucidate the relationship of DNA damage in pathophysiology of atherosclerosis and endothelial dysfunction in patients with stable CAD.
Prevalence of vitamin D insufficiency in children with forearm fractures
Saglam, Y (Saglam, Y.); Kizildag, H (Kizildag, H.); Toprak, G (Toprak, G.); Alp, NB (Alp, N. B.); Yalcinkaya, EY (Yalcinkaya, E. Y.)
JOURNAL OF CHILDRENS ORTHOPAEDICS, 11:3, 180-184, DOI: 10.1302/1863-2548.11.160008, 2017
Özet için tıklayınızPurpose This study aimed to determine whether children with distal radius impaction fractures have increased prevalence of 25-hydroxyvitamin D (25(OH) D) insufficiency compared with healthy controls.
Patients and Methods This is a prospective controlled study. The 30 children who were diagnosed with forearm fracture at the orthopaedic emergency clinic were included in the study and 30 healthy children from the routine paediatric outpatient unit were included as the control group. Peripheric venous 25(OH) D, calcium (Ca), magnesium (Mg), phosphor (P), alkaline phosphatase (ALP) and parathyroid hormone (PTH) of both groups were ed. The sample size was estimated based on the effect size for a type I error of 5% and power of 80%.
Results Demographic characteristics of the two groups did not differ in terms of weekly physical activity levels and breast milk intake. The mean whole body BMI was similar in both groups of patients. History of previous fracture and 25(OH) D level were significantly lower in the patient group than the control group.
Conclusion In the present study, the prevalence of vitamin D insufficiency or deficiency was higher in patients with forearm impaction type fractures than healthy controls and the baseline levels reported in the literature. In addition, there were no significant differences in serum Ca, Mg, P, ALP and PTH levels between the healthy controls and the patient group.
Auditory changes in acromegaly
Tabur, S (Tabur, S.); Korkmaz, H (Korkmaz, H.); Baysal, E (Baysal, E.); Hatipoglu, E (Hatipoglu, E.); Aytac, I (Aytac, I.); Akarsu, E (Akarsu, E.)
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 40:6, 621-626, DOI: 10.1007/s40618-016-0602-x, 2017
Özet için tıklayınızObjective The aim of this study is to determine the changes involving auditory system in cases with acromegaly.
Materials and methods Otological examinations of 41 cases with acromegaly (uncontrolled n = 22, controlled n = 19) were compared with those of age and gender-matched 24 healthy subjects. Whereas the cases with acromegaly underwent examination with pure tone audiometry (PTA), speech audiometry for speech discrimination (SD), tympanometry, stapedius reflex evaluation and otoacoustic emission tests, the control group did only have otological examination and PTA. Additionally, previously performed paranasal sinus-computed tomography of all cases with acromegaly and control subjects were obtained to measure the length of internal acoustic canal (IAC).
Results PTA values were higher (p < 0.001 for right ears and p = 0.001 for left ears), and SD scores were (p = 0.002 for right ears and p = 0.002 for left ears) lower in acromegalic patients. IAC width in acromegaly group was narrower compared to that in control group (p = 0.03 for right ears and p = 0.02 for left ears). When only cases with acromegaly were taken into consideration, PTA values in left ears had positive correlation with growth hormone and insulin-like growth factor-1 levels (r = 0.4, p = 0.02 and r = 0.3, p = 0.03). Of all cases with acromegaly 13 (32%) had hearing loss in at least one ear, 7 (54%) had sensorineural type and 6 (46%) had conductive type hearing loss.
Conclusion Acromegaly may cause certain changes in the auditory system in cases with acromegaly. The changes in the auditory system may be multifactorial causing both conductive and sensorioneural defects.
Synthesis, structural characterization, and antiproliferative/cytotoxic effects of a novel modified poly(maleic anhydride-co-vinyl acetate)/doxorubicin conjugate
Karakus, G (Karakus, Gulderen); Ece, A (Ece, Abdulilah); Yaglioglu, AS (Yaglioglu, Ayse Sahin); Zengin, HB (Zengin, Haci Bayram); Karahan, M (Karahan, Mesut)
POLYMER BULLETIN, 74:6, 2159-2184, DOI: 10.1007/s00289-016-1821-1, 2017
Özet için tıklayınızDrug carrier, poly(maleic anhydride-co-vinyl acetate) (MAVA or poly[MA-co-VA]) copolymer, was traditionally synthesized by free radical chain polymerization reaction, in methyl ethyl ketone (MEK) organic media at 80 A degrees C, using benzoyl peroxide (BPO) as the radicalic initiator. The purified copolymer was then modified with a chemotherapeutic agent, doxorubicin hydrochloride (DOX) at 75 A degrees C for 72 h, using N-(3-dimethyl-aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDAC) as the carboxylic acid-activating agent. Structural characterization of the MAVA and the modified MAVA/DOX conjugate was carried out by Fourier transform infrared (FTIR) and nuclear magnetic resonance (H-1-NMR and C-13-NMR). Their molecular weights were determined by size-exclusion chromatography (SEC). The spectroscopic and SEC results confirmed that conjugated/modification reaction was successfully carried out. UV spectrophotometric measurements indicated that MAVA/DOX preserved its molecular stability in physiological body fluid, PBS (physiological pH 7.40 at 37 A degrees C). Antiproliferative activities of MAVA/DOX were determined by BrdU cell proliferation ELISA assay using C6 (Rat Brain tumor cells) and HeLa (human uterus carcinoma) cell lines in vitro by comparing with free DOX agent (reference compound). Although MAVA showed low antiproliferative activity, both MAVA/DOX and DOX exhibited greater activity against HeLa and C6. Lactate dehydrogenase (LDH) leakage assay was performed for MAVA/DOX and DOX, which detected a non-toxic effect against C6 even at the highest dose (100 mu g/mL). IC50 and IC75 values were also determined using ED50 plus v1.0. Molecular modeling at M06-L/6-31 + G(d,p)//AM1 level showed that the electron density in MAVA/DOX is more localized resulting a higher polarization and thereby a higher dipole moment which shed light on the solubility of MAVA/DOX conjugate.
Extended locoregional use of intercostal artery perforator propeller flaps
Baghaki, S (Baghaki, Semih); Diyarbakirlioglu, M (Diyarbakirlioglu, Murat); Sahin, U (Sahin, Ugur); Kucuksucu, MA (Kucuksucu, Muge Anil); Turna, A (Turna, Akif); Baca, B (Baca, Bilgi); Aydin, Y (Aydin, Yagmur)
MICROSURGERY, 37:4, 293-299, DOI: 10.1002/micr.30098, 2017
Özet için tıklayınızBesides conventional flaps, intercostal artery perforator flaps have been reported to cover trunk defects. In this report the use of anterior intercostal artery perforator (AICAP) flap, lateral intercostal artery perforator (LICAP) flap and dorsal intercostal artery perforator (DICAP) flap for thoracic, abdominal, cervical, lumbar and sacral defects with larger dimensions and extended indications beyond the reported literature were reevaluated. Thirty-nine patients underwent surgery between August 2012 and August 2014. The age of the patients ranged between 16 and 79 with a mean of 49 years. The distribution of defects were as follows; 12 thoracic, 8 parascapular, 3 cervical, 8 abdominal, 4 sacral and 4 lumbar. AICAP, LICAP and DICAP flaps were used for reconstruction. Fifty-two ICAP flaps were performed on 39 patients. Flap dimensions ranged between 6 x 9 cm and 14 x 35 cm. Twenty-six patients had single flap coverage and 13 patients had double flap coverage. Forty-six flaps have been transferred as propeller flaps and 6 flaps have been transferred as perforator plus flap. Forty flaps (75%) went through transient venous congestion. In one DICAP flap, 30% of flap was lost. No infection, hematoma or seroma were observed in any patient. Follow-up period ranged between 3 and 32 months with a mean of 9 months. The ICAP flaps provide reliable and versatile options in reconstructive surgery and can be used for challenging defects in trunk.
Comparison of arch width changes following orthodontic treatment with and without extraction using three-dimensional models
Oz, AA (Oz, A. A.); Oz, AZ (Oz, A. Z.); Yazicioglu, S (Yazicioglu, S.); Arici, N (Arici, N.); Ozer, M (Ozer, M.); Arici, S (Arici, S.)
NIGERIAN JOURNAL OF CLINICAL PRACTICE, 20:5, 581-586, DOI: 10.4103/1119-3077.181389, 2017
Özet için tıklayınızObjective: To compare the arch width changes in patients treated fixed orthodontic mechanics without extraction (Group 1), with upper and lower first premolar extractions (Group 2), and with upper first premolar extraction only (Group 3). Materials and Methods: The study was conducted with pre- and post-treatment digital models from 240 patients. Anterior, middle, and posterior distances were measured on pre- and post-treatment models. At T1 measurements, the distance among the canine cusp tips, the second premolar buccal cusp tips, and the first molar mesiobuccal cusp tips were measured. In addition, the distance (D) between the intercanine and intermolar lines and the distance (D') between the interpremolar and intermolar lines were defined on the anatomic y-axis, and this distance was maintained in calculating posttreatment measurements (T2). Mandibular and maxillary arch width changes were evaluated within and between groups. Results: Anterior, middle, and posterior arch widths increased significantly in Groups 1 and 3. Maxillary anterior and middle arch widths also increased in Group 2, but the increases were not statistically significant. Changes in maxillary anterior and middle arch widths were higher in Groups 1 and 3 when compared to Group 2. However, there was no statistically significant difference in mandibular arch changes between the groups. Conclusion: Extraction treatment mechanics did not cause narrow dental arches, but nonextraction treatment increased arch width in all 3 measurements. Treatments with only upper arch extraction showed similar results with nonextraction treatment.
Diagnostic Value of Presepsin in Detection of Early-Onset Neonatal Sepsis
Ozdemir, AA (Ozdemir, Abdurrahman A.); Elgormus, Y (Elgormus, Yusuf)
AMERICAN JOURNAL OF PERINATOLOGY, 34:6, 550-556, DOI: 10.1055/s-0036-1593851, 2017
Özet için tıklayınızObjectiveThe aim of this study was to evaluate the efficacy of presepsin in both diagnosis and follow-up of early-onset neonatal sepsis (EOS) and also to compare its effectiveness with C-reactive protein (CRP) and procalcitonin (PCT).
MethodsA total of 29 term infants with EOS group and 40 term infants with control group were included in this study. Before initiating therapy, blood samples for whole blood count, CRP, PCT, presepsin, and culture were obtained from all neonates (T0). This procedure was repeated two times at 72 hours (T3) and day 7 (T7).
ResultsPresepsin levels of sepsis group at T0 were significantly higher (704.27223.54 pg/mL) than the control group (508.33 +/- 165.46 pg/mL). The sensitivity of CRP, PCT, and presepsin at T0 was found to be 83, 67, and 80%, whereas the specificity was found to be 75, 67, and 75%, respectively. The cutoff value for presepsin was 539 pg/mL with an area under the curve of 0.772.
ConclusionPresepsin may be used as a reliable and accurate marker for both diagnosis and follow-up of EOS. However, to increase the accuracy, presepsin may be used in combination with other markers such as CRP and PCT.
The importance of the neutrophil-to-lymphocyte ratio in patients with hypertrophic cardiomyopathy
Ozyilmaz, S (Ozyilmaz, Sinem); Akgul, O (Akgul, Ozgur); Uyarel, H (Uyarel, Huseyin); Pusuroglu, H (Pusuroglu, Hamdi); Gul, M (Gul, Mehmet); Satilmisoglu, MH (Satilmisoglu, Muhammet Hulusi); Bolat, I (Bolat, Ismail); Ozyilmaz, I (Ozyilmaz, Isa); Ucar, H (Ucar, Hakan); Yildirim, A (Yildirim, Aydin); Bakir, I (Bakir, Ihsan)
REVISTA PORTUGUESA DE CARDIOLOGIA, 36:4, 239-246, DOI: 10.1016/j.repc.2016.09.014, 2017
Özet için tıklayınızIntroduction: Previous studies have demonstrated the predictive value of the neutrophil-to-lymphocyte ratio (NLR) in many cardiovascular disorders. The aim of this study was to assess whether NLR is associated with echocardiographic or electrocardiographic parameters, or with predicted five-year risk of sudden cardiac death (SCD), in patients with hypertrophic cardiomyopathy (HCM).
Methods: This prospective observational study included 74 controls and 97 HCM patients. Three years of follow-up results for HCM patients were evaluated.
Results: NLR was significantly higher in patients with fragmented QRS, ventricular tachycardia, and presyncope than in those without (p=0.031, 0.030, and 0.020, respectively). NLR was significantly higher in patients whose predicted five-year risk of SCD was more than 6% and whose corrected QT interval was greater than 440 ms (p=0.022 and 0.001, respectively). It was also significantly higher in patients whose left ventricular ejection fraction (LVEF) was <60% than in those with LVEF >60% (p=0.017).
Conclusion: NLR was significantly higher in patients with HCM compared to the control group. A high NLR is associated with a higher five-year risk of SCD in patients with HCM. (C) 2017 Sociedade Portuguesa de Cardiologia. Basım Tarihi by Elsevier Espana, S.L.U. All rights reserved.
Serotonergic mechanisms of trigeminal meningeal nociception: Implications for migraine pain
Kilinc, E (Kilinc, Erkan); Guerrero-Toro, C (Guerrero-Toro, Cindy); Zakharov, A (Zakharov, Andrey); Vitale, C (Vitale, Carmela); Gubert-Olive, M (Gubert-Olive, Max); Koroleva, K (Koroleva, Ksenia); Timonina, A (Timonina, Arina); Luz, LL (Luz, Liliana L.); Shelukhina, I (Shelukhina, Irina); Giniatullina, R (Giniatullina, Raisa); Tore, F (Tore, Fatma); Safronov, BV (Safronov, Boris V.); Giniatullin, R (Giniatullin, Rashid)
NEUROPHARMACOLOGY, 116, 160-173, DOI: 10.1016/j.neuropharm.2016.12.024, 2017
Özet için tıklayınızSerotonergic mechanisms play a central role in migraine pathology. However, the region-specific effects of serotonin (5-HT) mediated via multiple types of receptors in the nociceptive system are poorly understood. Using extracellular and patch-clamp ings, we studied the action of 5-HT on the excitability of peripheral and central terminals of trigeminal afferents. 5-HT evoked long-lasting TTX-sensitive firing in the peripheral terminals of meningeal afferents, the origin site of migraine pain. Cluster analysis revealed that in majority of nociceptive fibers 5-HT induced either transient or persistent spiking activity with prevailing delta and theta rhythms. The 5-HT3-receptor antagonist MDL-72222 or 5-HT1BID-receptor antagonist GR127935 largely reduced, but their combination completely prevented the excitatory pro-nociceptive action of 5-HT. The 5-HT3 agonist mCPBG activated spikes in MDL-72222-dependent manner but the 5HT-1 receptor agonist sumatriptan did not affect the nociceptive firing. 5-HT also triggered peripheral CGRP release in meninges, which was blocked by MDL-72222.5-HT evoked fast membrane currents and Ca2+ transients in a fraction of trigeminal neurons. Immunohistochemistry showed expression of 5-HT3A receptors in fibers innervating meninges. Endogenous release of 5-HT from degranulated mast cells increased nociceptive firing. Low pH but not histamine strongly activated firing. 5-HT reduced monosynaptic inputs from trigeminal A delta- and C-afferents to the upper cervical lamina I neurons and this effect was blocked by MDL-72222. Consistent with central inhibitory effect, 5 HT reduced CGRP release in the brainstem slices. In conclusion, 5-HT evokes powerful pro-nociceptive peripheral and anti-nociceptive central effects in trigeminal system transmitting migraine pain. (C) 2016 Elsevier Ltd. All rights reserved.
Bonding performance of universal adhesives to er, cr:YSGG laser-irradiated enamel
Ayar, MK (Ayar, Muhammet Kerim); Erdemir, F (Erdemir, Fatih)
MICROSCOPY RESEARCH AND TECHNIQUE, 80:4, 387-393, DOI: 10.1002/jemt.22807, 2017
Özet için tıklayınızUniversal adhesives have been recently introduced for use as self-etch or etch-and-rinse adhesives depending on the dental substrate and clinical condition. However, their bonding effectiveness to laser-irradiated enamel is still not well-known. Thus, the aim of this study was to compare the shear bond strength (SBS) of universal adhesives (Single Bond Universal; Nova Compo-B Plus) applied to Er, Cr: YSGG laser-irradiated enamel with SBS of the same adhesives applied in self-etch and acid-etching modes, respectively. Crown segments of sixty bovine incisors were embedded into standardized acrylic blocks. Flattened enamel surfaces were prepared. Specimens were divided into six groups according to universal adhesives and application modes randomly (n=10), as follows: Single Bond Universal/acid-etching mode; Nova Compo-B Plus/acid-etching mode; Single Bond Universal/self-etching mode; Nova Compo-B Plus/self-etching mode; and Single Bond Universal/Er, Cr: YSGG Laser-etching mode; Nova Compo-B Plus/Er, Cr: YSGG Laser-etching mode. After surface treatments, universal adhesives were applied onto surfaces. SBS was determined after storage in water for 24 h using a universal testing machine with a crosshead speed of 0.5 mm min(-1). Failure modes were evaluated using a stereomicroscope. Data was analyzed using two-way of analyses of variances (ANOVA) (p=0.05). Two-way ANOVA revealed that adhesive had no effect on SBS (p=0.88), but application mode significantly influenced SBS (p=0.00). Acid-etching significantly increased SBS, whereas there are no significant differences between self-etch mode and laser-etching for both adhesives. The bond strength of universal adhesives may depend on application mode. Acid etching may significantly increase bond strength, while laser etching may provide similar bond strength when compared to self-etch mode.
Sociodemographic Characteristics and Career Decisions of Students in Dental School of Turkish Foundation University.
Ayar, MK (Ayar, Muhammet Kerim)
RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES, 8:2, 1770-1775, 2017
Özet için tıklayınızThe purposes of this study were to determine the sociodemographic characteristics of students enrolled in a dental school of Turkish foundation university and their motives for the choice of dentistry. Questionnaires were distributed to second and third year students of Biruni University Faculty of Dentistry. Questionnaire included items about their sociodemographic characteristics and their motives for the choice of dentistry. 124 of 163 returned the survey for an overall response rate of 76.1 percent. Of the respondents, 76 (61.3 percent) were females and 48 (38.7 percent) were males; they had an average age of 20.38 +/- 2.1. Personal decision had the greatest influence on the students in choosing dentistry as a career followed by first degree relative (father and mother). Fathers and mothers had similar impact on the students' choice of dental profession (46.0 percent and 52.1 percent, respectively). Relatives or family friends and a teacher in high school had more influence on female students' choice than those of male students significantly, respectively. Reputation and lifestyle provided by the profession and finding a job easily, were the predominant reasons that motivated both males and females to become a dentist. In conclusion, the reasons dental students enrolled in this study for choosing dentistry as a profession were primarily related to reputation and lifestyle provided by the profession, then economic.
Inhibition of poly(ADP-ribose) polymerase may have preventive potential for varicocoele-associated testicular damage in rats
Celik-Ozenci, C (Celik-Ozenci, C.); Kuscu, N (Kuscu, N.); Gungor-Ordueri, NE (Gungor-Ordueri, N. E.); Tasatargil, A (Tasatargil, A.); Sahin, P (Sahin, P.); Durmus, H (Durmus, H.)
ANDROLOGY, 5:2, 362-369, DOI: 10.1111/andr.12305, 2017
Özet için tıklayınızVaricocele is ordinarily accompanied by testicular damage and male infertility. Several theories have been proposed to explain the detrimental effect of varicocele on testis tSayı, including the possible effects of oxidative stress. The poly(ADP-ribose) polymerase (PARP) pathway has been established as a major downstream intracellular pathway of oxidative stress. Recently we have reported that PARP pathway has been activated in varicocele-induced rat testicular damage model. The aim of the present study was to investigate the possible protective effect of PARP inhibition in varicocele-associated testicular damage. Fifty male Wistar rats were divided into five groups: control, sham, varicocele-induced, varicocele-induced 1,5-isoquinolinediol (ISO, a PARP inhibitor)-treated, and ISO treated groups. The ISO-treated rats received intraperitoneal injections of 3 mg/kg ISO daily for 13 weeks. After 13 weeks of varicocele induction, body and testes weights were investigated in all groups. Histopathology of testes were evaluated by light microscopy. Expressions of PAR, p53 and cytochrome c were detected by immunohistochemistry and cleaved PARP-1, PAR, p53 and cytochrome c by western blot. The degree of apoptosis was determined by TUNEL. Light microscopy revealed testicular damage comprising various degrees of seminiferous tubule degeneration in varicocele-induced rats and their testes weights decreased significantly, whereas ISO administration prevented it. Expressions of cleaved PARP-1, PAR, cytochrome c, and p53 increased significantly in varicoceleinduced rats, whereas the level of these molecules were similar to controls in varicocele-induced rats treated with ISO. In conclusion, increased PARP activation in testes seems to be related with testicular damage and apoptosis associated with varicocele and pharmacological inhibition of this pathway might be an effective intervention to prevent varicocele-induced testicular injury.
Synthesis, characterization, preliminary SAR and molecular docking study of some novel substituted imidazo[2,1-b][1,3,4]thiadiazole derivatives as antifungal agents
Er, M (Er, Mustafa); Erguven, B (Erguven, Bugracan); Tahtaci, H (Tahtaci, Hakan); Onaran, A (Onaran, Abdurrahman); Karakurt, T (Karakurt, Tuncay); Ece, A (Ece, Abdulilah)
MEDICINAL CHEMISTRY RESEARCH, 26:3, 615-630, DOI: 10.1007/s00044-017-1782-4, 2017
Özet için tıklayınızThe aim of this study was to synthesize imidazo[2,1-b][1,3,4]thiadiazole derivatives, characterize them with various spectroscopic methods and investigate their antifungal activities. 2-Imino-1,3,4-thiadiazole derivatives 2a, b were synthesized by reacting nitrile compounds 1a, b with thiosemicarbazide (yields 75 and 88%). We then synthesized imidazo[2,1-b][1,3,4]thiadiazole derivatives 4-21, the target compounds, from the reactions of 2-amino-1,3,4-thiadiazole derivatives 2a, b with phenacyl bromide derivatives 3 (yields 52-69%). The structures of all synthesized compounds were characterized by infrared, H-1 nuclear magnetic resonance, C-13 nuclear magnetic resonance, elemental analysis and mass spectroscopy and X-ray diffraction analysis was also used for the compounds 7, 8, 10, and 17. Subsequently, in vitro antifungal activity tests were applied to all synthesized compounds. Inhibition zones, percentages of inhibition and LD50 doses were determined. Most of the synthesized compounds exhibited good antifungal activity against plant pathogens. Molecular docking and electronic properties calculations were carried out in order to see the potential binding conformations of the ligands and the effect of the substituents on the activities. Docking score successfully reflects the activity of the most active compound 10, which was found to have the lowest octanol/water partition coefficient and high HOMO energy value. The combination of experimental and computational work show that all the synthesized compounds have promising activities and might serve as novel drug candidates.
Radiopacity of bulk fill flowable resin composite materials
Yildirim, T (Yildirim, T.); Ayar, MK (Ayar, M. K.); Akdag, MS (Akdag, M. S.); Yesilyurt, C (Yesilyurt, C.)
NIGERIAN JOURNAL OF CLINICAL PRACTICE, 20:2, 200-204, DOI: 10.4103/1119-3077.178919, 2017
Özet için tıklayınızObjectives: The purpose of this study was to evaluate the radiopacity of currently marketed bulk fill flowable dental composite materials (Beautifil Bulk Flowable, SDR Flow, Filtek Bulk Fill Flow, and x-tra Base Bulk Fill).
Materials and Methods: Six specimens of each material with a thickness of 1 mm were prepared, and digital radiographs were taken, using a CCD sensor along with an aluminum stepwedge and 1 mm-thick tooth slice. The mean gray level of each aluminum stepwedge and selected materials was measured, using the equal-density area tool of Kodak Dental Imaging software. The equivalent thickness of aluminum for each material was then calculated by using the stepwedge values in the CurveExpert version 1.4 program.
Results: The radiopacity of bulk fill flowable composites sorted in descending order as follows: Beautifil Bulk Flowable (2.96 mm Al) = x-tra base bulk fill (2.92 mm Al) = SureFil SDR Flow (2.89 mm Al) > Filtek Bulk Fill Flow (2.51 mm Al) (P < 0.05).
Conclusions: As all materials had a radiopacity greater than dentin and enamel; their adequate radiopacity will help the clinicians during radiographic examination of restorations. Clinical Significance: Bulk fill composite materials have greater radiopacity, enabling clinicians to distinguish the bulk fill composites from dentin and enamel.
Magnetic Probe-Guided Excision of Nonpalpable Neck Lesions: A New and Safe Technique for Operated Neck Compartments
Aksakal, N (Aksakal, Nihat); Ozturk, A (Ozturk, Alper); Tutal, F (Tutal, Firat); Dogan, S (Dogan, Selim); Agcaoglu, O (Agcaoglu, Orhan); Bulakci, M (Bulakci, Mesut); Barbaros, U (Barbaros, Umut); Erbil, Y (Erbil, Yesim)
SURGICAL INNOVATION, 24:1, 42-48, DOI: 10.1177/1553350616676444, 2017
Özet için tıklayınızBackground: Reoperative neck surgery is technically more demanding because of the presence of scar tSayı and distorted anatomy. We aimed to investigate the magnetic probe-guided excision of nonpalpable neck lesions in patients with previously operated neck compartments. Methods: This study included 9 patients with recurrent/persistent thyroid carcinoma, recurrent/persistent hyperparathyroidism with previously operated neck compartments. The pathologic lesions were localized by ultrasonography, and magnetic tracer (0.2 mL, iron oxide) was injected directly into the pathologic lesions. Careful dissection was carried out following the area of maximum magnetic activity until the nonpalpable lesions were identified and excised. Result: All neck lesions were removed in 9 patients. The median count from lesion was significantly higher than values from lesion bed (background activity; (9900/5 seconds vs 250/5 seconds, P < .001). During follow-up, all patients had negative ultrasonography. Conclusion: Magnetic probe-guided technique could provide access to nonpalpable lesion localization in centers without readily available access to nuclear medicine facilities.
The effects of 17 beta-estradiol on blood brain barrier integrity in the absence of the estrogen receptor alpha; an in-vitro model
Kuruca, SE (Kuruca, Serap Erdem); Karadenizli, S (Karadenizli, Sabriye); Akgun-Dar, K (Akgun-Dar, Kadriye); Kapucu, A (Kapucu, Aysegul); Kaptan, Z (Kaptan, Zulal); Uzum, G (Uzum, Gulay)
ACTA HISTOCHEMICA, 119:6, 638-647, DOI: 10.1016/j.acthis.2017.07.005 Basım Tarihi: 2017
Özet için tıklayınızThe blood-brain barrier (BBB), which saves the brain from toxic substances, is formed by endothelial cells. It is mainly composed of tight junction (TJ) proteins existing between endothelial cells. Estrogen is an important regulatory hormone of BBB permeability. It protects the BBB before menopause, but may increase BBB permeability with aging. In addition, nitric oxide modulates BBB permeability. Alcohol impairs the integrity of the BBB with oxidants and inflammatory mediators such as iNOS. We investigated the effects of estrogen on BBB integrity in an in vitro BBB model created with ER alpha-free HUVEC (human umbilical vein endothelial-like cells) to mimics the menopausal period.
In vitro BBB model is created with HUVEC/C6 (rat glioma cells) co-culture. The effect of 17 beta-estradiol on ethanol-induced BBB disruption and change/or increase of iNOS activity, which modulate BBB integrity, were evaluated. Inducibility and functionality of BBB were investigated using transendothelial electrical resistance (TEER) and the expression of proteins TJ proteins (occludin and claudin-1) and iNOS activity by immunostaining.
Our results revealed that 17 beta-estradiol treatment before and after ethanol decrease expression of occludin and claudin-1 and value of TEER which are BBB disrupt indicators. In addition, ethanol and 17 beta-estradiol separately and pre-and post-ethanol 17 beta-estradiol treatment increased iNOS expression. Thus our study suggests caution in the use of 17 beta-estradiol after menopause because 17 beta-estradiol at this time may both increase the inflammatory process as well as damage the BBB. We think that beneficial effects of 17 beta-estradiol may be through ER alpha but it needs further studies.
Assessment of Nutritional Status and Metabolic Syndrome in Peritoneal Dialysis Patients: A Pilot Study
Tek, NA (Tek, Nilufer Acar); Sanlier, N (Sanlier, Nevin); Akbulut, G (Akbulut, Gamze); Onec, K (Onec, Kursad); Erten, Y (Erten, Yasemin)
GAZI MEDICAL JOURNAL, 28:3, 191-195, DOI: 10.12996/gmj.2017.56 Basım Tarihi: 2017
Özet için tıklayınızAim: Nutritional deficiencies and metabolic syndrome ( MS) is challenge in chronic kidney disease patients with peritoneal dialysis(PD). This study was planned in order to assess the nutritional status and metabolic syndrome in PD patients.
Methods: This study was performed on clinically stable patients that were undergoing PD therapy. Energy and nutrient intakes were determined with consequently three days dietary . The statement of MS was identified according to MS criteria adopted for PD patients.
Results: The prevalence of MS was found 64.3%. The mean duration of PD in with-MS(35.4 +/- 25.23mo.) was lower than without- MS(44.3 +/- 30.99mo.) (p> 0.05). Patients with MS had significantly greater systolic/ diastolic blood pressure and lower high- density- lypoprotein cholesterol levels (HDL-C) ( p< 0.05). According to the body mass index (BMI), the 83.3% of patients' with- MS and 30% without- MS were found overweight and body fat mass was significant higher in patients with- MS(p< 0.05). The mean daily protein intakes per kilograms of body weight determined lower with- MS patients (0.8 +/- 0.25g/kg) than without- MS (0.9 +/- 0.29 g/kg).
Conclusion: The prevalence of MS is remarkably high. The daily dietary energy and protein intakes were found under the recommended levels. Inadequate energy and protein intake increase loss of muscle mass and also excessive energy intake leads to obesity. Monitoring of nutritional status of PD patients is important both in prevention and progression of MS.
The Effects of 1,3,5-trisubstituted Indole Derivatives on Cell Growth, Apoptosis and MMP-2/9 mRNA Expression of MCF-7 Human Breast Cancer Cells
Bakar, F (Bakar, Filiz); Kilic-Kurt, Z (Kilic-Kurt, Zuhal); Caglayan, MG (Caglayan, Mehmet Gokhan); Olgen, S (Olgen, Sureyya)
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 17:5, 762-767, DOI: 10.2174/1871520616666160802115737 Basım Tarihi: 2017
Özet için tıklayınızBackground: Matrix metalloproteinases are known as extracellular matrix degrading enzymes and have important role on tumor progression.
Objective: This study reports the effects of 1,3,5-trisubstituted indole derivatives on cytotoxicity, apoptosis and MMP2/MMP-9 mRNA expression of MCF-7 human breast carcinoma cells.
Method: The cytotoxic effects of the compounds on MCF-7 cells were performed by MTT test, and cell proliferation was determined via BrdU incorporation. The apoptotic effects were observed by cell death detection elisa. The effects of the compounds on MMP-2/-9 enzyme activity and mRNA expression were also performed.
Results: The compounds inhibited the proliferation of MCF-7 breast carcinoma cells significantly in a dose dependent manner. All compounds were able to induce DNA fragmentation, especially compound 1. The IC50 values of compound 2 and 4 for MMP-2 were 0.42 mu M and 1.88 mu M, respectively. MMP-2 mRNA expression results were correlated with the inhibition of enzyme activity, such compound 4 inhibited MMP-2 mRNA expression at all treated concentrations. Docking simulation has also been performed to analyze the binding mode of compounds and the results showed that compound 2, the most active compound, formed a hydrogen bond with Glu202 for binding to the MMP-2 active site. In addition, the hydrophobic parts of compound 2 are in contact with nonpolar surface areas of MMP-2, such as His201, His211, Tyr223 and Tyr193.
Conclusion: According to the molecular docking results along with the biological assay data, it is suggested that compound 2 might be used for further design and development of MMP-2 inhibitors.
Evaluation of Biochemical Composition of Vitreous of Eyes of Diabetic Patients Using Proton Magnetic Resonance Spectroscopy
Simsek, IB (Simsek, Ilke Bahceci); Artunay, O (Artunay, Ozgur)
CURRENT EYE RESEARCH, 42:5, 754-758, DOI: 10.1080/02713683.2016.1242754 Basım Tarihi: 2017
Özet için tıklayınızPurpose: To investigate possible metabolic changes in vitreous of patients with diabetes mellitus with or without diabetic retinopathy using proton magnetic resonance spectroscopy.
Material and methods: Twenty-eight healthy control subjects (group 1), 18 patients with diabetes mellitus without diabetic retinopathy (group 2) and 18 patients with diabetic retinopathy (group 3) were included. Proton magnetic resonance spectroscopy was performed in left vitreous of all subjects; amounts of Glutamate, Choline, Lactate and Creatine values were measured. Choline/Creatine, Glutamate/Creatine and Lactate/Creatine ratios were calculated.
Results: Glutamate/Creatine and Lactate/Creatine ratios were significantly higher in group 2 and 3 when compared to group 1. Both Glutamate/Creatine and Lactate/Creatine ratios did not show any difference in group 2 when compared to group 3.
Conclusions: Our results suggest that when compared to healthy subjects, Glutamate and Lactate are the main metabolites of vitreous in diabetes mellitus patients. This may help us to better understand the metabolic factors in pathogenesis of diabetic retinopathy.
Bond strength of glass carbomer material to enamel and dentin following different surface pretreatments
Ayar, MK (Ayar, Muhammet Kerim); Guven, ME (Guven, Mehmet Esad)
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, 31:17, 1929-1937, DOI: 10.1080/01694243.2017.1289830 Basım Tarihi: 2017
Özet için tıklayınızObjective: Enamel and dentin bond strengths of restorative glass carbomer material were determined by shear bond strength (SBS) test after different surface treatments in this study.Materials and methods: Flat enamel and dentin surfaces pre-treated with 37% phosphoric acid (15s for enamel and dentin), or 20% polyacrylic acid (15s for enamel and dentin). Glass carbomer applied to the treated and non-treated surfaces. Conventional glass ionomer without any surface treatment served as a control.Results: Enamel and dentin SBSs of the conventional glass ionomer cement were significantly higher than those of the glass carbomer material bonded to enamel and dentin without any surface treatments. Acid-etching and polyacrylic acid pre-treatments yielded similar enamel bond strength to that of glass ionomer cement. For dentin bonding, only polyacrylic acid pre-treatment improved SBS of glass carbomer to dentin surface.Conclusions: Clinicians may consider the use of polyacrylic acid conditioner prior to the use of glass carbomer material.
Lack of association between increased mitochondrial DNA(4977) deletion and ATP levels of sputum cells from chronic obstructive pulmonary disease patients versus healthy smokers
Karimova, A (Karimova, A.); Oltulu, YM (Oltulu, Y. M.); Azakli, H (Azakli, H.); Kara, M (Kara, M.); Ustek, D (Ustek, D.); Tutluoglu, B (Tutluoglu, B.); Onaran, I (Onaran, I.)
MITOCHONDRIAL DNA PART A, 28:3, 361-369, DOI: 10.3109/19401736.2015.1126826 Basım Tarihi: 2017
Özet için tıklayınızIn this study we looked at smokers with and without chronic obstructive pulmonary disease (COPD) patients in order to evaluate the incidence of 4977 base pair (bp) mtDNA (mtDNA(4977)) deletion and mtDNA copy number in sputum cells and in peripheral blood leukocytes (PBLs) in relation to mitochondrial function and oxidative stress status. Twenty-five COPD patients who were current smokers, 22 smokers and 23 healthy nonsmokers (for only PBLs studies) participated in this study. The 4977-bp deletion was detected in all examined samples within 40 cyles of PCR amplification, using a quantitative real time PCR. The frequency of the mtDNA(4977) was significantly higher in the sputum cells of patients with COPD compared to smokers without COPD (p<0.0001). This difference was not observed in PBLs. Levels of cellular oxidative stress were significantly higher in the sputum cells of subjects with COPD than in the smoker group. However, mtDNA copy number, mitochondrial membrane potential (Delta Psi m) and cellular ATP levels in PBLs and sputum cells were not significantly different between the studied groups. The Pearson analysis revealed no correlations between the accumulation of mtDNA(4977), and intracellular ATP content and Delta Psi m values of the sputum cells, although there was a positive correlation between the increase in the percentage of deleted mtDNA(4977) and the levels of cellular oxidative stress in COPD patients (r = 0.80, p<0.0001). Our studies may suggest that the accumulation of mtDNA(4977) in the sputum cells of smokers with COPD does not seem to have an important impact on mitochondrial dysfunction in relation to ATP production and Delta Psi m when compared to those of healthy smokers.
Approaches to Cope with Stress and Depression in Individuals with Urinary Incontinence
Bilgic, D (Bilgic, Dilek); Beji, NK (Beji, Nezihe Kizilkaya); Ozbas, A (Ozbas, Ayfer); Cavdar, I (Cavdar, Ikbal); Yalcin, O (Yalcin, Onay)
GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 82:2, 181-187, DOI: 10.1159/000446955 Basım Tarihi: 2017
Özet için tıklayınızAim: The aim of study was to assess individuals with urinary incontinence (UI) with respect to depression and to determine coping mechanisms with stress. Methods: One hundred sixty female and 110 male participants that applied to Istanbul Faculty of Medicine, Urology and Urogynecology Department with UI complaints and accepted to participate in the study were included in the study. Depressive symptoms were assessed with Center for Epidemiological Studies Depression Scale (CES-D). The mechanisms of coping with stress were evaluated using Ways of Coping with Stress Inventory (WCSI). Results: Females (57.5%) scored 16 points and more from the CES-D scale, while the rate was significantly higher in males (79.1%). The scores obtained in 5 subdimensions of the WCSI showed that females utilized a desperate approach (female 1.39 +/- 0.63, male 1.11 +/- 0.51, p < 0.000), self-confident approach (female 1.98 +/- 0.60, male 1.70 +/- 0.42, p < 0.000), and social support approach (female 1.90 +/- 0.57, male 1.48 +/- 0.44, p < 0.000) statistically and significantly more than males in coping with stress. Conclusion: Males experienced more depression symptoms when compared to females. Females were significantly more self-confident and utilized social support mechanisms and desperate approaches more than males in order to cope with stress. (C) 2016 S. Karger AG, Basel
Keratoacanthoma in an acrochordon
Aksoy, B (Aksoy, Berna); Aksoy, HM (Aksoy, Hasan Mete); Akin, O (Akin, Onat)
TURKDERM-ARCHIVES OF THE TURKISH DERMATOLOGY AND VENEROLOGY, 51:1, 24-25, DOI: 10.4274/turkderm.85698 Basım Tarihi: 2017
A cross-sectional study to evaluate metabolic and demographic factors affecting cognitive function among low educated internal medicine outpatients
Alakus, F (Alakus, Feride); Helvaci, SA (Helvaci, Serife A.); Temizel, M (Temizel, Mustafa); Arman, Y (Arman, Yucel)
NEUROSCIENCES, 22:1, 38-43, DOI: 10.17712/nsj.2017.1.20160259 Basım Tarihi: JAN 2017
Özet için tıklayınızObjective: To evaluate factors affecting cognitive function in internal medicine outpatients.
Methods: A total of 130 consecutive outpatients aged 50-80 years old were included in this cross-sectional study conducted at Okmeydani Training and Research Hospital, Istanbul, Turkey between March and May 2013. Cognitive function was evaluated via Standardized Mini-Mental State Examination (SMMSE) scores. Logistic regression analysis was performed to determine factors predicting poor cognitive function.
Results: Mild-to-moderate cognitive impairment was noted in 39.2% of the patients. Median (interquartile range) total SMMSE scores were significantly higher in patients aged <= 60 than > 60 years (27.0 (2.0) vs. 25.0 (5.0), p=0.000). Multivariate linear regression analyses revealed female gender (B, -1.27; 95% CI, -2.36 to -0.18; p=0.023) and aging (B, -0.20; 95% CI, -0.26 to -0.14; p< 0.001) to result in a significant decrease in the total SMMSE scores.
Conclusions: Mild-to-moderate cognitive impairment was observed in 39.2% of internal medicine outpatients. Old age and female gender were significant predictors of lower total SMMSE scores. Furthermore, besides for high language scores in hypertensive patients on combined therapy, no significant impact of hypertension or obesity was observed on the SMMSE scores.
Biomechanical comparison of orthogonal versus parallel double plating systems in intraarticular distal humerus fractures
Atalar, AC (Atalar, Ata C.); Tunali, O (Tunali, Onur); Ersen, A (Ersen, Ali); Kapicioglu, M (Kapicioglu, Mehmet); Saglam, Y (Saglam, Yavuz); Demirhan, MS (Demirhan, Mehmet S.)
ACTA ORTHOPAEDICA ET TRAUMATOLOGICA TURCICA, 51:1, 23-28, DOI: 10.1016/j.aott.2016.11.001 Basım Tarihi: 2017
Özet için tıklayınızObjectives: In intraarticular distal humerus fractures, internal fixation with double plates is the gold standard treatment. However the optimal plate configuration is not clear in the literature. The aim of this study was to compare the biomechanical stability of the parallel and the orthogonal anatomical locking plating systems in intraarticular distal humerus fractures in artificial humerus models.
Methods: Intraarticular distal humerus fracture (AO13-C2) with 5 mm metaphyseal defect was created in sixteen artificial humeral models. Models were fixed with either orthogonal or parallel plating systems with locking screws (Acumed elbow plating systems). Both systems were tested for their stiffness with loads in axial compression, varus, valgus, anterior and posterior bending. Then plastic deformation after cyclic loading in posterior bending and load to failure in posterior bending were tested. The failure mechanisms of all the samples were observed.
Results: Stiffness values in every direction were not significantly different among the orthogonal and the parallel plating groups. There was no statistical difference between the two groups in plastic deformation values (0.31 mm-0.29 mm) and load to failure tests in posterior bending (372.4 N-379.7 N). In the orthogonal plating system most of the failures occurred due to the proximal shaft fracture, whereas in the parallel plating system failure occurred due to the shift of the most distal screw in proximal fragment.
Conclusion: Our study showed that both plating systems had similar biomechanical stabilities when anatomic plates with distal locking screws were used in intraarticular distal humerus fractures in artificial humerus models. (C) 2016 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.
Synthesis and Antioxidant Activity of Indole Derivatives Containing 4-Substituted Piperazine Moieties
Altuntas, TG (Altuntas, Tunca Gul); Yilmaz, N (Yilmaz, Nilufer); Coban, T (Coban, Tulay); Olgen, S (Olgen, Sureyya)
LETTERS IN DRUG DESIGN & DISCOVERY, 14:4, 380-386, DOI: 10.2174/1570180813666161020165623 Basım Tarihi: 2017
Özet için tıklayınızBackground: Oxidative stress caused by reactive oxygen species (ROS) plays a significant role in many human disorder such as cancer, rheumatoid arthritis, atherosclerosis, stroke, neurodegeneration, and diabetes as well as the process of aging. Compounds interfere with the action of ROS might be useful in prevention and treatment of these pathologies. Indole nucleus seems to protect the nervous system against oxidative stress.
Methods: Herein, several indole compounds containing 4-substituted piperazine moieties were synthesized. Compounds 1, and 4-15 were obtained by amidification reaction using 1,1'-carbonyldiimidazole (CDI) and compounds 2 and 3 were obtained by the reaction of amine and substituted chloropyridine ring using potassium carbonate. Antioxidant activities of compounds were determined by in vitro lipid peroxidation levels on rat liver, superoxide formation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) stable radical scavenging activities in comparison with vitamine E (alpha-tocopherol).
Results: The results indicated that compounds 6 (75%), 7 (66%), 8 (75%), 11 (88%), and 12 (69%) showed strong inhibiory effects on superoxide radical formation compare to vitamine E (62%). Compound 11 can be considered as the most active one among them. Compound 10 (38%) showed slight activity in lipid peroxidation inhibition assay. Compounds are not good inhibitor of DPPH except compound 10, which has also slight inhibition (20%).
Assessment of Aflatoxin M1 and Heavy Metal Levels in Mothers Breast Milk in Famagusta, Cyprus
Kunter, I (Kunter, Imge); Hurer, N (Hurer, Nazife); Gulcan, HO (Gulcan, Hayrettin Ozan); Ozturk, B (Ozturk, Baris); Dogan, I (Dogan, Irfan); Sahin, G (Sahin, Gonul)
BIOLOGICAL TRACE ELEMENT RESEARCH, 175:1, 42-49, DOI: 10.1007/s12011-016-0750-z Basım Tarihi: JAN 2017
Özet için tıklayınızBreast milk contributes towards optimal nutrition for infants. However, studies showed that it can also contain different toxins and heavy metals, which reduce its health benefits. The aim of this study is to determine the level of contaminants such as aflatoxin M1 (AFM1), Pb, Cd, As, and Hg in breast milk samples from Famagusta, Cyprus. Correlations between moldy food consumption, smoking habits of the mothers, and contaminant levels in breast milk were also investigated. Breast milk samples from 50 lactating mothers in rural and urban areas of Famagusta District were analyzed for AFM1 by ELISA. Eighty percent of them were found to be contaminated with AFM1 with the mean measurement of 7.84 +/- 1.72 ng/l. Socio-demographic status, moldy food consumption habits, and smoking status do not have any effect on the AFM1 levels observed in breast milk. Heavy metal levels in breast milk were examined by inductively coupled plasma mass spectrometry, and the mean measurements were1.19 +/- 1.53 ppm for Pb, 0.73 +/- 0.58 ppm for As, 0 +/- 0.20 ppm for Hg, and 0.45 +/- 0.23 ppm for Cd. This study indicates that the levels of these contaminants in breast milk samples obtained in Famagusta District are well within the acceptable levels. However, the presence of AFM1 and heavy metals still may pose risks for infant health.
Oral mucosa and lung cancer: Are genetic changes in the oral epithelium associated with lung cancer?
Komerik, N (Komerik, N.); Yuce, E (Yuce, E.); Calapoglu, NS (Calapoglu, N. S.); Kosar, PA (Kosar, P. A.); Cakir, M (Cakir, M.); Koparan, G (Koparan, G.)
NIGERIAN JOURNAL OF CLINICAL PRACTICE, 20:1, 12-18, DOI: 10.4103/1119-3077.181396 Basım Tarihi: JAN 2017
Özet için tıklayınızAim: To compare genetic aberrations in the oral epithelium of lung cancer patients with those without cancer.
Subjects and Methods: Buccal smears were performed to collect oral epithelium from each of the participants (smoker cancer patients n = 50, smoker control subjects n = 40, and nonsmoker control subjects n = 25). Cytogenetic changes in the samples were detected by micronuclei assay, whereas p53 and murine double minute 2 (MDM2) polymorphisms were genotyped using polymerase chain reaction-restriction fragment length polymorphism.
Results: p53 codon 72 polymorphism was seen in 44% of cancer patients versus 12.5% in smokers and 12% in nonsmokers of the control group. Similarly, MDM2 single nucleotide polymorphism 309 polymorphism was seen in 34% of patients with lung cancer as opposed to 12.5% of smokers (P = 0.038) and 8% of nonsmokers (P = 0.019) of the control group.
Conclusion: A higher proportion of individuals with lung cancer demonstrate genetic damage to oral mucosa compared to those without cancer.
Effects of Er,Cr:YSGG Laser Pulse Frequency on Microtensile Bond Strength to Enamel
Ayar, MK (Ayar, M. K.); Yildirim, T (Yildirim, T.)
OPERATIVE DENTISTRY, 42:1, 53-61, DOI: 10.2341/15-193-L Basım Tarihi: JAN 2017
Özet için tıklayınızLiterature regarding the influence of Er,Cr:YSGG laser pulse frequency with different output power levels on adhesion properties of adhesive resin to lased enamel is limited. Therefore, the aim of the present study was to evaluate the effects of laser pulse frequency (20, 35, and 50 Hz) at two different output power settings (3 and 6 W) of Er,Cr:YSGG on the microtensile bond strength (lTBS) of adhesive resin to enamel. Crowns of 35 intact bovine incisors were embedded into self-cure acrylic resin individually, and then flat enamel surfaces were prepared with 600-grit silicon carbide papers under water cooling. Teeth were divided randomly into seven groups. Enamel surfaces were irradiated with Er,Cr:YSGG laser operated at one of six output power-pulse frequency combinations (6 W20 Hz, 6 W-35 Hz, 6 W-50 Hz, 3 W-20 Hz, 3 W-35 Hz, and 3W-50 Hz) in groups 1-6, respectively. Bur-treated surfaces served as a control in group 7. After surface treatments and bonding procedures, composite build-ups were done in three layers up to a height of 4 mm. Next, all bonded teeth were sectioned into the resin-enamel sticks to be tested in a lTBS testing machine. The mu TBS data were analyzed with univariate analysis of variance under a general linear model with the factor 'tooth' added as a random effect to the design. Resin-enamel interfaces were evaluated with scanning electron microscopy (SEM). The lTBS to laser-irradiated enamel in group 1 (6 W-20 Hz) was significantly lower than those of bur-treated enamel (p<0.05). However, group 6 (3 W-50 Hz) showed significantly higher mu TBS values than did bur-treated teeth (p<0.05). SEM evaluation revealed enormous morphological alterations of laser-irradiated specimens, such as extensive vertical and horizontal microcracks and gaps, with the exception of group 6. The bonding effectiveness of adhesive resin to laser-irradiated enamel was affected by the pulse frequen- cy of the Er,Cr:YSGG laser. Although the parameters recommended by the manufacturer lowered mu TBS, increasing the pulse rate may maintain optimum mu TBS.
Could Heterozygous Beta Thalassemia Provide Protection Against Multiple Sclerosis?
Cikrikcioglu, MA (Cikrikcioglu, Mehmet Ali); Ozcan, ME (Ozcan, Muhammed Emin); Halac, G (Halac, Gulistan); Gultepe, I (Gultepe, Ilhami); Celik, K (Celik, Kenan); Sekin, Y (Sekin, Yahya); Eser, EE (Eser, Elif Ece); Burhan, S (Burhan, Sebnem); Cetin, G (Cetin, Guven); Uysal, O (Uysal, Omer)
MEDICAL SCIENCE MONITOR, 22, 4854-4858, DOI: 10.12659/MSM.898192, 11 2016
Özet için tıklayınızBackground: Heterozygous beta thalassemia (HBT) has been proposed to increase the risk of developing autoimmune disease. Our aim in this study was to examine the prevalence of HBT among multiple sclerosis (MS) patients.
Material/Methods: HBT frequency was investigated in our MS group (243 patients with MS). Hemoglobin electrophoresis (HE) was carried out if MS patients had a mean corpuscular Cilt No of (MCV) <80 fL and a mean corpuscular hemoglobin level of (MCH) <27 pg/L according to a complete blood count (CBC). If MCV was lower than 80 fL, MCH was lower than 27 pg/L, and Hemoglobin A2 equal to or higher than 3.5%, a diagnosis of HBT was established. The frequency of patients with HBT in our MS patient group was statistically compared with the prevalence of HBT in the city of Istanbul, where our MS patients lived.
Results: The HBT prevalence was 0.823% (2 patients) in the MS patient group. The prevalence of HBT in Istanbul has been reported to be 4.5%. According to the z-test, the HBT prevalence in our MS patient group was significantly lower than that in Istanbul (Z=6.3611, two-sided p value <0.0001, 95% confidence interval of prevalence of HBT in our MS patient group: 0.000998-0.029413).
Conclusions: Contrary to our hypothesis at the outset of study, the reduced HBT prevalence in the MS group compared to HBT frequency in the city of Istanbul might indicate that HBT is protective against MS.
The Effect of Urinary Incontinence on Sexual Quality of Life in Women with Chronic Physical Diseases
Yilmaz, FT (Yilmaz, Feride Taskin); Kumsar, AK (Kumsar, Azime Karakoc); Demirel, G (Demirel, Gulbahtiyar); Yesildag, B (Yesildag, Birnur)
SEXUALITY AND DISABILITY, 34:4, 403-415, DOI: 10.1007/s11195-016-9457-5, 2016
Özet için tıklayınızThis study was conducted in order to determine the effect of the presence of urinary incontinence on sexual quality of life in women diagnosed with chronic physical diseases. The sample of the cross sectional and comparative study consisted of 67.5 % (n = 191) female inpatients with and 32.5 % (n = 92) female inpatients without urinary incontinence complaints being treated for chronic physical diseases in the internal diseases clinic of a public hospital. Data was collected using the patient identification form, the International Incontinence Consultation Questionnaire-Short Form and the Sexual Quality of Life Scale for Women. The average score that women with urinary incontinence took from the sexual quality of life scale (61.50 +/- 19.10) was lower than the average score of women without urinary incontinence (77.19 +/- 18.75) (p < 0.01). There was a negative meaningful relationship between the frequency, amount, and effect of urinary incontinence and sexual quality of life (p < 0.05). The sexual quality of life of women who were 50 years of age and above, who had respiratory system diseases, had a duration of illness between 6 and 10 years, perceived their general health as bad, parity and gave normal birth, were in their menopausal period, and had hysterectomies was significantly lower (p < 0.05). As a result, in women with chronic physical diseases and urinary incontinence, the sexual quality of life is affected in a worse level compared to those who do not have urinary incontinence.
The Role of Adult Hippocampal Neurogenesis in Learning and Memory Function
Kaptan, Z (Kaptan, Zulal); Uzum, G (Uzum, Gulay)
TURKISH JOURNAL OF NEUROLOGY, 22:4, 149-155, DOI: 10.4274/tnd.68889, 2016
Özet için tıklayınızAdult hippocampal neurogenesis is the lifelong production of new functional neurons from neural stem cells in the adult brain. Production of neurons in the adult brain is limited to two areas. One of these is the subgranular region in the hippocampus dentate gyrus (DG), the other is the subventricular zone of the lateral ventricles. New neurons produced in the hippocampus DG functionally integrate into mature circuits, and contribute to cognition and emotional control, learning and memory functions in particular. It is still unclear how that contribution occurs and a key Sayı that is being intensively investigated. Hippocampal neurogenesis declines both with age and in neurodegenerative diseases characterized by deterioration of memory. Understanding the contribution of hippocampal neurogenesis to memory function and investigating factors that affect neurogenesis have gained importance for protective or therapeutic approaches against these neurodegenerative diseases. It is suggested that new neurons produced in the DG contribute to spatial and episodic memory function by pattern separation (prevent overlapping of similar information by making separate presentations) through their electrophysiologic properties (being stimulated easier than mature neurons) in the maturation process. Hippocampal neurogenesis is important in episodic and spatial memory formation. In this review we will try to explain how hippocampal neurogenesis occurs, factors that affect the neurogenesis process, and the role of neurogenesis in memory function based on the present literature.
The effect of health-promoting lifestyle education on the treatment of unexplained female infertility
Kaya, Y (Kaya, Yeliz); Beji, NK (Beji, Nezihe Kizilkaya); Aydin, Y (Aydin, Yunus); Hassa, H (Hassa, Hikmet)
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 207, 109-114, DOI: 10.1016/j.ejogrb.2016.10.050, 2016
Özet için tıklayınızObjective: This study aimed to reveal the 1) awareness, 2) improvements of a health-promoting lifestyle on women with unexplained infertility having at least one of the risk factors that have been indicated to negatively affect fertility (smoking, body mass index lower than 18.5 kg/m(2) and more than 25 kg/m(2), over-exercising or not exercising at all, alcohol consumption, caffeine consumption of more than 300 mg/day, and high levels of stress) by means of health-promoting lifestyle education, 3) the effect of this improvement on the result of assisted-reproduction treatment in terms of clinical pregnancy.
Study design: 64 women diagnosed with unexplained infertility were divided into a group receiving Health-Promoting Lifestyle (HPL) education and a control group. 1) Risk Factors Questionnaire (BMI, Smoking, Alcohol, Stress, Exercise, Caffeine), 2) Depression, Anxiety and Stress Scale, 3) Health Promoting Lifestyle Profile II. The health promoting lifestyle was given to the education group. The Risk Factors Questionnaire; Depression, Anxiety, Stress Scale and Healthcare-Promoting Lifestyle Profile II were also administered after the first-second-third month of education but before ART treatment.
Results: A statistically significant decrease was found in the average levels of four variables as; BMI (p < 0.001) -stress (p < 0.001)-caffeine consumption (p <0.001)-lower exercise levels (p < 0.001). Moreover, the total number of risk factors that females had between the first and third interview decreased significantly. Clinical pregnancy rate after ART was 12 (46.1%) and 5 (19.2%) in education and control group consequently (p = 0.02).
Conclusion: Health-promoting lifestyle education was found to be effective in reducing the lifestyle risk factors for infertility and increasing the success rates of assisted reproduction treatment by correcting these risk factors. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
The relationship of self-perception, personality and high school type with the level of problematic internet use in adolescents
Ozturk, E (Ozturk, Ebru); Ozmen, SK (Ozmen, Suna Kaymak)
COMPUTERS IN HUMAN BEHAVIOR, 65, 501-507, DOI: 10.1016/j.chb.2016.09.016, 2016
Özet için tıklayınızAs adolescents spend a substantial part of the day using internet, they constitute one of the risk groups for problematic internet use. The purpose of this study was to investigate the relationship between problematic internet use and the self-perception, personality type, gender of adolescents, and how these relationships varied depending on the type of high school the students attended. This study was conducted with 771 students studying at Anatolian high schools, science high schools, arts high schools, vocational high schools for girls, vocational technical high schools, trade vocational high schools, and common high schools in the city of Kars, Turkey. In this study, 433 (56.2%) of the participants were girls, while 338 (43.8%) were boys. The forms, scales and questionnaires administered during the study included the Personal Information Form, the Problematic Internet Use Scale, the Self-Perception Scale for Adolescents, and Eysencic's Personality Questionnaire. Data were analyzed using multiple linear regression analysis and the one way ANOVA test. The present study examined whether personality type (extraversion, neuroticism, lie and psychoticism), self-perception (romantic appeal, physical appearance, social acceptance/close friendship, behavioral conduct, athletic competence and job competence), gender, and duration of internet use can predict problematic internet use. Based on the study results, it was observed that gender, duration of internet use, lying type personality, neuroticism, psychoticism, romantic appeal, physical appearance and behavioral conduct were significant predictors of problematic internet use, while positive attributes such as extraversion, athletic competence, job competence, and social acceptance/close friendship were not. Moreover, it was determined that the type of high school led to significant differences with regards to problematic internet use among students, and that students at technical high school were more prone to problematic internet use. (C) 2016 Elsevier Ltd. All rights reserved.
Receptor discordance rate and its effects on survival in primary and recurrent breast cancer patients
Ilgun, S (Ilgun, Serkan); Sarsenov, D (Sarsenov, Dauren); Erdogan, Z (Erdogan, Zeynep); Ordu, C (Ordu, Cetin); Celebi, F (Celebi, Filiz); Pilanci, KN (Pilanci, Kezban Nur); Ozturk, A (Ozturk, Alper); Selamoglu, D (Selamoglu, Derya); Alco, G (Alco, Gul); Aktepe, F (Aktepe, Fatma); Eralp, Y (Eralp, Yesim); Tuzlali, S (Tuzlali, Sitki); Ozmen, V (Ozmen, Vahit)
JOURNAL OF BUON, 21:6, 1425-1432, 2016
Özet için tıklayınızPurpose: The receptor status of breast cancer plays a critical role in clinical practice. During the metastatic process, a change in the biological characteristics of the tumor can be seen. This study aimed to investigate the hormone receptor and HER2 status changes between primary and recurrent breast cancers and their effect on survival.
Methods: Eighty-six breast cancer patients with biopsy-proven local recurrences or distant metastases during the follow-up period were included in the study. Patients with metastatic disease at the time of first diagnosis or with history of previous neoadjuvant chemotherapy were excluded.
Results: Forty-three of the 86 patients (50%) had changes in at least one of the estrogen receptor (ER), progesterone receptor (PR), or HER2. ER, PR and HER2 discordance rates were 12.7,38.3, and 15.1%, respectively, and PR discordance was significantly higher (p=0.000). Among all molecular subtypes, the triple negative breast cancer (TNBC) subtype showed the least change. When the effect of chemotherapy on receptor change was analyzed, PR discordance was significantly higher in the group who received chemotherapy (p =0.029). Analysis of the hormonotherapy effects on receptor discordance revealed results similar to those of chemotherapy. Only the PR discordance was significantly greater in the group that received hormonotherapy (p=0.000).
None of the three receptor discordances or loss of any receptor were related to survival. Primary tumor TNBC subtype and disease-free-interval (DFI) shorter than 5 years were found as independent prognostic factors that negatively affected overall survival (OS).
Conclusion: This study showed that during recurrent disease there was 50% discordance in the expression of ER, PR, and HER2. The receptor showing the greatest discordance and influence from the systemic treatment was PR. A significant relationship between receptor discordance and survival could not be demonstrated in our study.
Efficacy of denture cleaners on the surface roughness and Candida albicans adherence of sealant agent coupled denture base materials
Koroglu, A (Koroglu, Aysegul); Sahin, O (Sahin, Onur); Dede, DO (Dede, Dogu Omur); Deniz, ST (Deniz, Sule Tugba); Sever, NK (Sever, Nurdan Karacan); Ozkan, S (Ozkan, Serkan)
DENTAL MATERIALS JOURNAL, 35:5, 810-816, DOI: 10.4012/dmj.2016-103, 2016
Özet için tıklayınızThis study investigated the effect of denture cleansers on the surface roughness and Candida albicans adherence of surface sealant agent coupled denture base resins. One hundred and twenty specimens were fabricated from 2 polymethyl methacrylate (PMMA) (Meliodent; Acron MC) and 1 polyamide (Deflex) denture base materials, coated with a sealant agent (Palaseal) and divided into 4 groups (n=10) according to overnight cleaning procedures: distilled water (control), 5% sodium hypochlorite (NaOCl) and two different sodium perborate (Corega; Rapident). The surface roughness values were measured with a profilometer before (R(a)0) and after 90 days immersion in denture cleaners (R(a)1). Specimens were incubated with Candida albicans suspension and Candida colony forming units (CFU) (Cfu/mm) were counted. Significant differences were found, between the R(a)0 and R(a)1 values of 5% NaOCl applied Acron MC, Deflex and also Rapident applied Deflex groups (p<0.05). Denture cleaning procedures had no significant effects on the quantitiy of Candida albicans.
Hemoglobins as new players in multiple sclerosis: metabolic and immune aspects
Altinoz, MA (Altinoz, Meric A.); Ozcan, EM (Ozcan, Emin M.); Ince, B (Ince, Bahri); Guloksuz, S (Guloksuz, Sinan)
METABOLIC BRAIN DISEASE, 31:5, 983-992, DOI: 10.1007/s11011-016-9845-7, 2016
Özet için tıklayınızBasic science investigations and clinical observations in recent years indicate that hemoglobins (Hbs) may have important roles in the pathogenesis of multiple sclerosis (MS). These findings can be summarized as follows: 1- Erythrocyte fragility is higher in MS patients, the released free Hb damages blood-brain barrier, myelin basic protein and also triggers iron overload and inflammation. 2- Free Hb may further activate the inflammatory responses through Toll-like receptor 4 (TLR4), present on microglia and other innate immunocytes. 3- Hbs are expressed in neural cells including dopaminergic neurons. Also, several studies have demonstrated that Hbs are expressed in astrocytes and oligodendroglia. 4- Hb overexpression in neural cells upregulate mitochondrial complex I-V subunits. The comparison of the mitochondrial proteome between healthy and patients with MS revealed only four differentially expressed proteins including Hb beta-chain. 5- Microarray analysis of 8300 genes in monocytes of twins with and without MS showed a difference in 25 genes that include genes encoding alpha- and beta-globins as well. 6- beta- and alpha-globin gene clusters reside at chromosomal regions 11p15.5 and 16p13.3, respectively. Whole genome screen (WGS) in Sardinian MS families using 327 markers revealed linkage in 3 regions including 11p15.5 loci. Further, 11p15.5 and 16p13.3 were part of the 17 regions identified in the WGS study of 136 sibling-pairs in Nordic countries analyzing 399 microsatellite markers. In the light of these findings, we propose that free Hb released from dying erythrocytes is detrimental. On the contrary, intracellular Hbs in neural cells are protective in MS. The genomic linkage findings can be explained by common haematologically-silent Hb variants that may lower the protective function of intracellular Hbs, and therefore, enhance the risk for MS. In the absence of such variants, aberrations in the translational and post-translational mechanisms controlling synthesis of neural Hbs may also enhance the vulnerability to MS. Alternatively, such genetic variants may perturb the metabolism of anti-inflammatory hemorphins produced via cleavage of Hbs.
A Meta-Analysis: Identification of Common Mir-145 Target Genes that have Similar Behavior in Different GEO Datasets
Pashaei, E (Pashaei, Elnaz); Guzel, E (Guzel, Esra); Ozgurses, ME (Ozgurses, Mete Emir); Demirel, G (Demirel, Goksun); Aydin, N (Aydin, Nizamettin); Ozen, M (Ozen, Mustafa)
PLOS ONE, 11:9 Article Number: e0161491, DOI: 10.1371/journal.pone.0161491, 21 2016
Özet için tıklayınızBackground
MicroRNAs, which are small regulatory RNAs, post-transcriptionally regulate gene expression by binding 3'-UTR of their mRNA targets. Their deregulation has been shown to cause increased proliferation, migration, invasion, and apoptosis. miR-145, an important tumor supressor microRNA, has shown to be downregulated in many cancer types and has crucial roles in tumor initiation, progression, metastasis, invasion, recurrence, and chemo-radioresistance. Our aim is to investigate potential common target genes of miR-145, and to help understanding the underlying molecular pathways of tumor pathogenesis in association with those common target genes.
Eight Basım Tarihi microarray datasets, where targets of mir-145 were investigated in cell lines upon mir-145 over expression, were included into this study for meta-analysis. Inter group variabilities were assessed by box-plot analysis. Microarray datasets were analyzed using GEOquery package in Bioconducter 3.2 with R version 3.2.2 and two-way Hierarchical Clustering was used for gene expression data analysis.
Meta-analysis of different GEO datasets showed that UNG, FUCA2, DERA, GMFB, TF, and SNX2 were commonly downregulated genes, whereas MYL9 and TAGLN were found to be commonly upregulated upon mir-145 over expression in prostate, breast, esophageal, bladder cancer, and head and neck squamous cell carcinoma. Biological process, molecular function, and pathway analysis of these potential targets of mir-145 through functional enrichments in PPI network demonstrated that those genes are significantly involved in telomere maintenance, DNA binding and repair mechanisms.
As a conclusion, our results indicated that mir-145, through targeting its common potential targets, may significantly contribute to tumor pathogenesis in distinct cancer types and might serve as an important target for cancer therapy.
Anti-Mullerian Hormone and Inhibin-A, but not Inhibin-B or Insulin-Like Peptide-3, may be Used as Surrogates in the Diagnosis of Polycystic Ovary Syndrome in Adolescents: Preliminary Results
Yetim, A (Yetim, Aylin); Yetim, C (Yetim, Cagcil); Bas, F (Bas, Firdevs); Erol, OB (Erol, Oguz Bulent); Cig, G (Cig, Gulnaz); Ucar, A (Ucar, Ahmet); Darendeliler, F (Darendeliler, Feyza)
JOURNAL OF CLINICAL RESEARCH IN PEDIATRIC ENDOCRINOLOGY, 8:3, 288-297, DOI: 10.4274/jcrpe.3253, 2016
Özet için tıklayınızObjective: Polycystic ovary syndrome (PCOS) is a common endocrine problem in adolescents with an increasing prevalence of 30%. Pursuing new biomarkers with high specificity and sensitivity in the diagnosis of PCOS in adolescents is currently an active area of research. We aimed to investigate the diagnostic value of anti-Mullerian hormone (AMH), insulin-like peptide-3 (INSL3), inhibin-A (INH-A), and inhibin-B (INH-B) in adolescents with PCOS and also to determine the association, if any, between these hormones and clinical/laboratory findings related with hyperandrogenism.
Methods: The study group comprised 53 adolescent girls aged between 14.5 and 20 years who were admitted to our outpatient clinic with symptoms of hirsutism and/or irregular menses and diagnosed as having PCOS in accordance with the Rotterdam criteria. Twenty-six healthy peers, eumenorrheic for at least two years and body mass index-matched, constituted the controls. Fasting blood samples for hormones [luteinizing hormone (LH), follicle-stimulating hormone (FSH), dehydroepiandrosterone-sulfate (DHEAS), androstenedione (D4-A), total/free testosterone (T/fT), sex hormone binding globulin (SHBG), AMH, INSL3, INH-A, INH-B] were drawn after an overnight fast.
Results: In the PCOS group, 83% of the subjects were oligomenorrheic/amenorrheic and 87% had hirsutism. The LH, LH/FSH ratio, total T, fT, free androgen-index (FAI), DHEAS levels were significantly higher (p=0.005, p=0.042, p=0.047, p<0.001, p=0.007, p=0.014, respectively) and SHBG was significantly lower (p=0.004) in PCOS patients as compared to the controls. Although the INSL3 and INH-B levels showed no difference between the groups (p>0.05), AMH and INH-A levels were found to be significantly higher in the PCOS group compared to the controls (p<0.001, p<0.001, respectively). In multiple linear regression analysis, WC SDS (p=0.028), logD4-A (p=0.033), logSHBG (p=0.031), and total ovarian Cilt No (p=0.045) had significant effects on AMH levels, and LH (p=0.003) on INH-A levels. In receiver-operating characteristic analysis, the cut-off values for AMH and INH-A were 6.1 ng/mL (sensitivity 81.1%) and 12.8 pg/mL (sensitivity 86.8%), respectively, to diagnose PCOS. When AMH and INH-A were used in combination, the sensitivity (96.2%) increased.
Conclusion: INSL3 and INH-B were not found to have diagnostic value in adolescents with PCOS. On the other hand, it was shown that INH-A could be used as a new diagnostic biomarker in addition to AMH.
Lower Urinary Tract Symptoms in Women With Type 2 Diabetes Mellitus
Yilmaz, SD (Yilmaz, Sema Dereli); Bal, MD (Bal, Meltem Demirgoz); Celik, S (Celik, Selda); Rathfisch, G (Rathfisch, Gulay); Beji, NK (Beji, Nezihe Kizilkaya); Dinccag, N (Dinccag, Nevin); Yalcin, O (Yalcin, Onay)
JOURNAL OF WOUND OSTOMY AND CONTINENCE NURSING, 43:5, 523-528, DOI: 10.1097/WON.0000000000000259 , 2016
Özet için tıklayınızPURPOSE: The aim of the study was to compare lower urinary tract symptoms (LUTS) in women with and without type 2 diabetes mellitus (DM).
SUBJECTS AND SETTING: The sample was drawn from community-dwelling women in the province of Istanbul who were cared for in the diabetes outpatient clinic of Istanbul Medical School between January and June 2012. Two hundred forty-nine women with DM were compared to 255 women without DM cared for in the obstetrics and gynecology department of the same university hospital. The mean ages of the groups were 55.1 and 53.7 years, respectively.
METHODS: Participants completed a questionnaire that queried sociodemographic and clinical characteristics; the Bristol Female Lower Urinary Tract Symptoms-Short Form (BFLUTS-SF) was used to evaluate LUTS. The questionnaire required 10 to 15 minutes to complete; participants completed the questionnaire in a private room of each of the respective outpatient clinics.
RESULTS: No statistically significant differences were found when groups (women with and without DM) were compared based on age and cigarette smoking (P > .05). In contrast, BMI scores were significantly higher in the women with DM (P < .001). The cumulative BFLUTS scores and the filling and incontinence symptoms subscale sores (P < .001) were significantly higher in women with DM. No differences were observed in voiding symptoms (P = .347), sexual function (P = .380), and health-related quality of life (P = .142) subscale scores. The prevalence of storage symptoms nocturia, voiding frequency, urge incontinence, stress incontinence, frequency of incontinent episodes were higher among women with DM. In addition, women with DM were more likely to report the need to change clothing because of urinary leakage, effect of incontinence on daily tasks, and overall interference with daily activities of living.
CONCLUSIONS: Women with type 2 DM are more likely to experience LUTS as compared to women without DM. Women with type 2 DM should routinely be assessed for LUTS.
Evaluation of microtensile and tensile bond strength tests determining effects of erbium, chromium: yttrium-scandium-gallium-garnet laser pulse frequency on resin-enamel bonding
Yildirim, T (Yildirim, T.); Ayar, MK (Ayar, M. K.); Yesilyurt, C (Yesilyurt, C.); Kilic, S (Kilic, S.)
NIGERIAN JOURNAL OF CLINICAL PRACTICE, 19:5, 585-590, DOI: 10.4103/1119-3077.188702 Basım Tarihi: SEP 2016
Özet için tıklayınızObjectives: The aim of the present study was to compare two different bond strength test methods (tensile and microtensile) in investing the influence of erbium, chromium: yttrium-scandium-gallium-garnet (Er, Cr: YSGG) laser pulse frequency on resin-enamel bonding. Materials and Methods: One-hundred and twenty-five bovine incisors were used in the present study. Two test methods were used: Tensile bond strength (TBS; n = 20) and micro-TBS (mTBS; n = 5). Those two groups were further split into three subgroups according to Er, Cr: YSGG laser frequency (20, 35, and 50 Hz). Following adhesive procedures, microhybrid composite was placed in a custom-made bonding jig for TBS testing and incrementally for mTBS testing. TBS and mTBS tests were carried out using a universal testing machine and a microtensile tester, respectively. Results: Analysis of TBS results showed that means were not significantly different. For mTBS, the Laser-50 Hz group showed the highest bond strength (P 0.05), and increasing frequency significantly increased bond strength (P 0.05). Comparing the two tests, the mTBS results showed higher means and lower standard deviations. Conclusion: It was demonstrated that increasing mTBS pulse frequency significantly improved immediate bond strength while TBS showed no significant effect. It can, therefore, be concluded that test method may play a significant role in determining optimum laser parameters for resin bonding.
The relationship between quadriceps strength and joint position sense, functional outcome and painful activities in patellofemoral pain syndrome
Guney, H (Guney, Hande); Yuksel, I (Yuksel, Inci); Kaya, D (Kaya, Defne); Doral, MN (Doral, Mahmut Nedim)
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 24:9, 2966-2972, DOI: 10.1007/s00167-015-3599-3, 2016
Özet için tıklayınızThe aim of this study was to investigate how strongly the concentric and eccentric quadriceps strengths were correlated with the joint position sense, functional outcomes and painful activities in patients with patellofemoral pain syndrome (PFPS).
The study included forty-six women diagnosed with unilateral PFPS. Eccentric and concentric quadriceps strengths were ed at 60 and 180A degrees/s. Active knee joint position sense (JPS) was measured at 20A degrees and 60A degrees of flexion. Functional levels were determined by using Kujala patellofemoral scores. Pain levels during stair descending and ascending, squatting and prolonged sitting were measured using 0-10 cm visual analogue scale. The relationship of isokinetic quadriceps strength with JPS results, Kujala score and pain levels were evaluated using Spearman's correlation coefficient test.
Eccentric and concentric quadriceps strengths were significantly lower on involved side than uninvolved side. JPS results were poorer on the painful knee when compared to uninvolved side. While eccentric strength correlated with both JPS target angles, concentric strength was correlated only with 20A degrees. Both eccentric and concentric strengths were significantly correlated with Kujala scores and pain levels.
Quadriceps eccentric strength was correlated more to joint position sense than concentric strength. Both eccentric and concentric quadriceps strength related to pain and functional level in PFPS patients.
Comparative Evaluation of Immediate Bond Strength to Enamel with One-Step Self-Etch Adhesives
Ayar, MK (Ayar, Muhammet Kerim)
RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES, 7:5, 2014-2019 Basım Tarihi: SEP 2016
Özet için tıklayınızAvailable knowledge in the literature regarding the immediate bond strength of one-step, self-etch adhesives to enamel surfaces covered with thick layers is limited. Therefore, the immediate bond strength of six one-step, self-etch adhesive systems to enamel surfaces covered with thick smear layers was evaluated. Flattened enamel surfaces were prepared and polished with 180-grit silicon carbide abrasive paper under running water for 20 seconds, to produce thick smear layers similar to clinical conditions. The following were applied to bonding surfaces: self-etch adhesives, six one-step, self-etch adhesives, Adper Prompt L-POP, iBond, Clearfil S3 Bond, AdheSE One F, G-Bond, Optibond All-in-one, one two-step, self-etch adhesive, and Clearfil SE Bond. Bonded teeth were sectioned into sticks immediately after bonding and subsequently subjected to microtensile bond strength testing. Differences in bond strength for enamel were determined using two-way ANOVA with Tukey tests. iBond, AdheSE One F, Optibond All-in-one failed to bond enamel surfaces covered with thick smear layers. However, Adper Prompt L-POP and Clearfil S3 Bond showed similar enamel bond strength to Clearfil SE Bond. It should be noted that the bonding effectiveness of the majority of one-step, self-etch adhesives tested to enamel surfaces covered with thick smear layers is poor.
Total hip arthroplasty in patients with ankylosing spondylitis: Midterm radiologic and functional results
Saglam, Y (Saglam, Yavuz); Ozturk, I (Ozturk, Irfan); Cakmak, MF (Cakmak, Mehmet Fevzi); Ozdemir, M (Ozdemir, Mustafa); Yazicioglu, O (Yazicioglu, Onder)
ACTA ORTHOPAEDICA ET TRAUMATOLOGICA TURCICA, 50:4, 443-447, DOI: 10.1016/j.aott.2016.06.010, 2016
Özet için tıklayınızIntroduction: The aim of this study was to evaluate the clinical and radiological outcomes of total hip arthroplasty (THA) in patients with Ankylosing Spondylitis (AS).
Patients and methods: One hundred five hips of 61 AS patients (mean age: 41.3 +/- 10.2 years) who underwent THA between 1997 and 2012 were included into the study. Dorr's classification of proximal femoral geometry, acetabular protrusio, bone ankylosis, acetabular protrusion, Brooker classification of heterotopic ossification (HO), Gruen and Charnley classifications of implant loosening were used in radiographic assessments. Patients were called back to return for an additional long-term follow-up for functional assessment.
Results: Cementless total hip arthroplasty was used in 83 hips (79%) and cemented TKA was used in 22 hips (21%). The overall rate of aseptic loosening was 7.6% at a mean follow-up of 5.4 years. Femoral loosening was statistically similar in cemented and cementless femoral components (14% vs. 8%, p = 0.089). Acetabular component loosening was statistically higher in patients with any degree of HO (p = 0.04). Regardless of the type of femoral implant (cemented or cementless), femoral component loosening was higher in Dorr's type C patients (p = 0.005). The average pre-operative HSS was 46.6 +/- 16.3, and it improved to 80.7 +/- 18.7 at last follow-up (p < 0.01).
Conclusion: Revision incidence was similar in between ankylosed and non-ankylosed hips. While complication rates are high, significant functional improvement can be achieved after THA in patients with AS. (C) 2016 Turkish Association of Orthopaedics and Traumatology. Publishing services by Elsevier B. V.
Gingival crevicular fluid interleukin-8 and lipoxin A(4) levels of smokers and nonsmokers with different periodontal status: a cross-sectional study
Lutfioglu, M (Lutfioglu, M.); Aydogdu, A (Aydogdu, A.); Sakallioglu, EE (Sakallioglu, E. E.); Alacam, H (Alacam, H.); Pamuk, F (Pamuk, F.)
JOURNAL OF PERIODONTAL RESEARCH, 51:4, 471-480, DOI: 10.1111/jre.12324, 2016
Özet için tıklayınızBackground and ObjectiveSmoking is an important risk factor for periodontal disease and effects the pathogenesis of the disease. This study evaluated the impact of smoking on gingival crevicular fluid interleukin-8 (IL-8) and lipoxin A(4) (LxA(4)) levels in patients with and without periodontal disease.
Material and MethodsA total of 122 participants were grouped as follows: smokers with generalized aggressive periodontitis (S-GAgP, n = 15); smokers with chronic periodontitis (S-CP, n = 17); smokers with gingivitis (SG, n = 15); smokers classified as periodontally healthy (SH, n = 15); nonsmokers with generalized aggressive periodontitis (N-GAgP, n = 15); nonsmokers with chronic periodontitis (N-CP, n = 15); nonsmokers with gingivitis (NG, n = 15); and nonsmokers classified as periodontally healthy (NH, n = 15). Gingival index, plaque index, probing pocket depth and clinical attachment level were ed. Gingival crevicular fluid IL-8 and LxA4 levels were analyzed by ELISA.
ResultsGingival crevicular fluid IL-8 levels varied among groups, as follows: S-GAgP>S-CP>SG>SH and N-GAgP>N-CP>NG>NH. The gingival crevicular fluid IL-8 levels were significantly higher in the S-GAgP group compared with the N-GAgP group and in the S-CP group compared with the N-CP group (p < 0.05); differences between the SG and NG and the SH and NH groups were not statistically significant (p > 0.05). Gingival crevicular fluid LxA(4) levels also varied among groups, but in an inverse direction when compared with the IL-8 levels, as follows: S-GAgP<S-CP<SG and N-GAgP<N-CP<NG. (The gingival crevicular fluid LxA(4) levels in SH and NH groups were below the limits of detection.) The gingival crevicular fluid LxA(4) levels were significantly lower in the S-GAgP group than in the N-GAgP group and in the S-CP group than in the N-CP group (p < 0.05); differences between the SG and NG groups were not statistically significant (p > 0.05).
ConclusionThe study findings suggest that the observed increases in gingival crevicular fluid IL-8 levels and decreases in gingival crevicular fluid LxA(4) levels reflect changes in immune and inflammatory responses that occur as a result of smoking.
Cyberbullying Tendencies of High School Students
Topaloglu, M (Topaloglu, Murat); Topaloglu, AO (Topaloglu, Aysegul Ozdemir)
INTERNATIONAL JOURNAL OF EDUCATIONAL SCIENCES, 15:1-2 Special Sayı: SI, 88-95 Basım Tarihi: JUL-AUG 2016
Özet için tıklayınızThe rapid developments in technology have affected human life in many ways. While technology makes life easier, it has been producing new concepts, which are just alternative ways of demonstrating violence, conflict and bullying. With individuals or groups starting to use information and communication technologies for the purpose of doing harm, the term cyberbullying has been introduced. One of the most effective ways of coping with cyberbullying is to raise awareness among individuals. Cyberbullying includes crimes such as harassment, humiliation, intimidation of a child or adolescent by another person using the Internet and mobile technologies. The aim of this study is to assess the cyberbullying tendencies of high school students. High school students make up the population of the study. The study sample comprises 300 high school students in the City of Edirne.
Differential expression of LOXL4 in normal and tumour tSayı samples of laryngeal squamous cell carcinoma
Yilmaz, M (Yilmaz, M.); Suer, I (Suer, I.); Karatas, OF (Karatas, O. F.); Cansiz, H (Cansiz, H.); Ozen, M (Ozen, M.)
CLINICAL OTOLARYNGOLOGY, 41:3, 206-210, DOI: 10.1111/coa.12498, 2016
Özet için tıklayınızObjective: Head and neck cancer is the sixth most common cancer in worldwide with an increasing incidence rate in recent years. LOXL4 is expressed in several tSayıs, and its expression has been shown to display a significant correlation with local lymph node metastasis. In this study, we aimed to explore the LOXL4 expression level in metastatic and non-metastatic LSCC tSayıs and to determine its prognostic significance.
Study design: Basic science research study.
Setting: University hospital.
Participants: A total of 40 patients were included in the study.
Main outcome measure: LOXL4 expression status in metastatic, non-metastatic LSCC and normal tSayı samples was investigated by quantitative reverse-transcription PCR.
Results: We demonstrated that LOXL4 was significantly overexpressed in LSCC tumour tSayı samples in comparison with the corresponding normal tSayıs (P < 0.001); however, no significant relationship has been found between LOXL4 expression and either the metastatic potential or the T classification of the specimens.
Conclusions: Although expression of LOXL4 is not statistically associated with neck metastases, we showed that LOXL4 expression significantly increased in laryngeal cancer.
A peer-assisted learning experience in computer programming language learning and developing computer programming skills
Altintas, T (Altintas, Tugba); Gunes, A (Gunes, Ali); Sayan, H (Sayan, Hamiyet)
INNOVATIONS IN EDUCATION AND TEACHING INTERNATIONAL, 53:3, 329-337, DOI: 10.1080/14703297.2014.993418, 3 2016
Özet için tıklayınızPeer learning or, as commonly expressed, peer-assisted learning (PAL) involves school students who actively assist others to learn and in turn benefit from an effective learning environment. This research was designed to support students in becoming more autonomous in their learning, help them enhance their confidence level in tackling computer programming problems as well as to encourage them towards team work and collective effort. The main purpose of this research is to test the effectiveness of PAL on the C programming language course in the second semester of undergraduate level. For this purpose, an experiment group (peer students) and a control group were statistically compared in terms of midterm and final exam scores and the results showed that peer students were more successful in C programming language.
The effect of home bleaching agents on the surface roughness of five different composite resins: A SEM evaluation
Cengiz, E (Cengiz, Esra); Kurtulmus-Yilmaz, S (Kurtulmus-Yilmaz, Sevcan); Ulusoy, N (Ulusoy, Nuran); Deniz, ST (Deniz, Sule Tugba); Yuksel-Devrim, E (Yuksel-Devrim, Ece)
SCANNING, 38:3, 277-283, DOI: 10.1002/sca.21307, 2016
Özet için tıklayınızThe aim of this study was to investigate the effect of hydrogen peroxide (HP) and carbamide peroxide (CP) on the surface roughness of five different composite resins using profilometer and scanning electron microscope (SEM). Thirty-six specimens (1mm thick, 10mm in diameter) of five composite resins were fabricated. Each composite group was equally divided into three subgroups as control, CP and HP. In control group, specimens were stored in daily refreshed distilled water during the 14-day testing period. In other groups, 10% HP (Opalescence Treswhite) and 10% CP (Opalescence PF) were applied and surface roughness values (Ra) of each specimen were measured with a profilometer at the end of 14 days. Additionally, SEM analysis was performed to evaluate the surface deformations of composite resins. Data were analyzed with Kruskal-Wallis and Mann-Whitney U tests. Ra values of composite groups exposed to bleaching agents were statistically higher than control group (p<0.05). There was no significant difference between Ra values after HP and CP application within each composite group while SEM micrographs showed higher surface alterations at HP group compared to CP. Among the composite resins tested, Ceram-X Mono revealed the lowest Ra values after CP and HP applications as seen at SEM images. Home bleaching agents increased the surface roughness of all composites. Except CP applied Ceram-X mono specimens, Ra values of all composite resins evaluated in this study exceeded the critical limit of 0.2m. Ceram-X mono was the least affected composite material after bleaching application. SCANNING 38:277-283, 2016. (c) 2016 Wiley Periodicals, Inc.
Effect of Different Composite Placement Molds on Resin-Enamel/Dentin Shear Bond Strength
Ayar, MK (Ayar, Muhammet Kerim); Yesilyurt, C (Yesilyurt, Cemal); Yildirim, T (Yildirim, Tahsin)
JOURNAL OF RESEARCH IN MEDICAL AND DENTAL SCIENCE, 4:2, 141-145, DOI: 10.5455/jrmds.20164213, 2016
Özet için tıklayınızBackground: Different types of molds are being used to place resin composite and build composite buildup in shear bond strength test. However, a little information is available in the literature regarding their effects on resin-enamel/dentin bond strengths.
Aims: The aim of the present study was to investigate the effect of different composite placement molds on shear bond strength (SBS) of composite resin bonded to enamel and dentin.
Methods & Material: Three different composite placement molds (silicone mold, one-piece fixed plexiglass mold, two-piece removable plexiglass) and two substrates (enamel and dentin) were used in this study. Composite resins were bonded to enamel and dentin surfaces with using one of the tested placement molds (n=20) and SBS tests were performed using universal testing machine.
Results: The findings showed that bond strength was not influenced by different composite placement molds (p=0.147), but influenced by bonding substrate (p=0.000). More cohesive failures in enamel were evident with one-piece fixed mold. It can be concluded that composite placement molds have no effect on resin-enamel and dentin bond strengths, but it affects failure mode distributions in resin-enamel bonding.
The effects of posture and relaxation training on sleep, dyspnea, pain and, quality of life in the short-term after cardiac surgery: a pilot study
Akinci, B (Akinci, Buket); Yeldan, I (Yeldan, Ipek); Bayramoglu, Z (Bayramoglu, Zehra); Akpinar, TB (Akpinar, Tahsin Belhhan)
TURK GOGUS KALP DAMAR CERRAHISI DERGISI-TURKISH JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, 24:2, 258-265, DOI: 10.5606/tgkdc.dergisi.2016.11675, 2016
Özet için tıklayınızBackground: This study aims to evaluate the effects of a two-week posture and relaxation training program on sleep quality, daytime sleepiness, dyspnea, pain, and quality of life in patients undergoing cardiac surgery.
Methods: Twenty-four patients were randomly assigned to the control (n=12) or posture and relaxation training group (n=12). Sleep quality, daytime sleepiness, dyspnea, quality of life, and pain were assessed using the Pittsburgh Sleep Quality Index, Epworth Sleepiness Scale, Modified Medical Research Council Dyspnea Scale, Euro Quality of Life-5D, and visual analog scale, respectively. The control group received a standard postoperative rehabilitation program, while the training group underwent an additional posture and relaxation training program. Both groups continued their rehabilitation program for one week at the hospital and one week at home.
Results: After treatment, there were statistically significant differences in the Pittsburgh Sleep Quality Index sum scores, subjective sleep quality, sleep medication use, sleep disturbance, and visual analog scale pain scores between the groups (p<0.05). The posture and relaxation training group showed a significant improvement in the Modified Medical Research Council scores, but not in Pittsburgh Sleep Quality Index sum scores. After treatment, a significantly higher number of patients showed improvements in the quality of life in the posture and relaxation training group (p<0.05). The Pittsburgh Sleep Quality Index, subjective sleep, and sleep medication use subgroup scores showed significant improvements in the posture and relaxation training group after treatment (p<0.05).
Conclusion: Posture and relaxation training has beneficial effects for maintaining sleep quality, reducing sleep medication use, alleviating dyspnea and pain, and increasing quality of life in cardiac surgery patients.
Identification of miR-139-5p as a saliva biomarker for tongue squamous cell carcinoma: a pilot study
Duz, MB (Duz, Mehmet Bugrahan); Karatas, OF (Karatas, Omer Faruk); Guzel, E (Guzel, Esra); Turgut, NF (Turgut, Nesrettin Fatih); Yilmaz, M (Yilmaz, Mehmet); Creighton, CJ (Creighton, Chad J.); Ozen, M (Ozen, Mustafa)
CELLULAR ONCOLOGY, 39:2, 187-193, DOI: 10.1007/s13402-015-0259-z, 2016
Özet için tıklayınızOf all human oral carcinomas, 41 % are localized to the tongue. Despite considerable improvements in both diagnosis and treatment, tongue squamous cell carcinoma (TSCC) has remained one of the most lethal types of cancer. Here, we aimed at identifying a salivary microRNA (miRNA) expression signature specific for TSCC patients.
To identify putative diagnostic biomarkers, we compared the miRNA expression profiles of saliva samples from three TSCC patients and four healthy control individuals using an Agilent miRNA microarray platform (V19). Three of the differentially expressed miRNAs identified were selected for further validation using quantitative reverse-transcription PCR (qRT-PCR) in saliva samples from 25 TSCC patients and 25 healthy control individuals.
Through microarray-based expression profiling, we found that 419 miRNAs were deregulated in the saliva samples from the TSCC patients compared to those from the healthy control individuals tested. Subsequent qRT-PCR analysis revealed that the expression level of miR-139-5p was significantly reduced in the TSCC validation samples compared to the controls. Further analysis of post-operative saliva samples derived from TSCC patients revealed that the miR-139-5p expression levels had turned back to normal again. In addition, we found that miR-139-5p exhibited enough power to discriminate pre-operative TSCC patients from both normal individuals (AUC: 0.805) and post-operative TSCC patients (AUC: 0.713), thereby underscoring its diagnostic potential.
From our results we conclude that saliva can be used as a feasible Dergi for routine TSCC diagnostics and that miR-139-5p may serve as a potential biomarker for early TSCC detection.
Assessing the effect of school bullying prevention programs on reducing bullying
Albayrak, S (Albayrak, Sevil); Yildiz, A (Yildiz, Ayse); Erol, S (Erol, Saime)
CHILDREN AND YOUTH SERVICES REVIEW, 63, 1-9, DOI: 10.1016/j.childyouth.2016.02.005, 2016
Özet için tıklayınızThis study was conducted for the purpose of assessing the effects on reducing bullying of a school bullying prevention program (BPP). A pre-test/post-test and control group, semi-experimental design was used for the research. The universe and sampling for the study comprised 583 pupils, ages 12-15, enrolled in two elementary schools in Istanbul. The BPP was prepared in accordance with the Behavioral-Ecological Model (BEM) and the Neuman Systems Model (NSM) and it encompassed faculty members, families and students. In the post-test, the mean scores of the study group in all of the sub-scales of the Peer Victimization Scale (PVS) and in the "threatening/intimidating" sub-scale of the Peer Bullying Behavior Scale (PBBS) were found to be significantly lower than those of the control group (p < .05). It was concluded that the BPP organized in line with the BEM and the NSM was effective in reducing bullying and could be used in nursing practices. (C) 2016 Elsevier Ltd. All rights reserved.
The role of ATP-binding cassette transporter genes in the progression of prostate cancer
Karatas, OF (Karatas, Omer F.); Guzel, E (Guzel, Esra); Duz, MB (Duz, Mehmet B.); Ittmann, M (Ittmann, Michael); Ozen, M (Ozen, Mustafa)
PROSTATE, 76:5, 434-444, DOI: 10.1002/pros.23137, 1 2016
Özet için tıklayınızBACKGROUNDProstate cancer (PCa) is the most commonly diagnosed neoplasm and the second leading cause of cancer-related death among men in developed countries. There is no clear evidence showing the success of current screening tests in reducing mortality of PCa. In this study, we aimed to profile expressions of nine ABC transporters, ABCA5, ABCB1, ABCB6, ABCC1, ABCC2, ABCC3, ABCC5, ABCC10, and ABCF2, in recurrent, non-recurrent PCa and normal prostate tSayıs.
METHODSA total of 77 (39 recurrent, 38 non-recurrent) radical prostatectomy and 20 normal prostate samples, obtained from Baylor College of Medicine Prostate Cancer program, were included into the study and divided into two independent groups as test and validation sample sets. Differential expression of selected ABC transporters was assessed using quantitative real-time PCR (qRT-PCR). Pearson's correlation test, receiver operating characteristics (ROC) analysis and Kaplan-Meier test were used for statistical analysis.
RESULTSQRT-PCR results demonstrated the elevated expression of ABCA5, ABCB1, ABCB6, ABCC1, and ABCC2 as well as reduced expression of ABCC3 in PCa samples compared to normal prostate tSayıs. In addition, we found deregulation of ABCB1, ABCB6, ABCC3, and ABCC10 in recurrent PCa samples and validated differential expression of ABCB6, ABCC3, and ABCC10 in recurrent PCa compared to non-recurrent PCa. Pearson's correlation, ROC and Kaplan-Meier analysis revealed the power of these three ABC transporters for estimating prognosis of PCa.
CONCLUSIONSWe demonstrated differential expression of ABC transporters both in tumor versus normal and recurrent versus non-recurrent comparisons. Our data suggest ABCB6, ABCC3, and ABCC10 as valuable predictors of PCa progression. Prostate 76:434-444, 2016. (c) 2015 Wiley Periodicals, Inc.
The effects of the sagittal plane malpositioning of the patella and concomitant quadriceps hypotrophy on the patellofemoral joint: a finite element analysis
Aksahin, E (Aksahin, Ertugrul); Kocadal, O (Kocadal, Onur); Aktekin, CN (Aktekin, Cem N.); Kaya, D (Kaya, Defne); Pepe, M (Pepe, Murad); Yilmaz, S (Yilmaz, Serdar); Yuksel, HY (Yuksel, H. Yalcin); Bicimoglu, A (Bicimoglu, Ali)
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 24:3, 903-908, DOI: 10.1007/s00167-014-3421-7, 2016
Özet için tıklayınızAnterior knee pain is a common symptom after intramedullary nailing in tibia shaft fracture. Moreover, patellofemoral malalignment is also known to be a major reason for anterior knee pain. Patellofemoral malalignment predisposes to increased loading in patellar cartilage. In the previous study, we have demonstrated the quadriceps atrophy and patellofemoral malalignment after intramedullary nailing due to tibia shaft fracture. In this study, our aim was to clarify the effects of quadriceps atrophy and patellofemoral malalignment with the pathologic loading on the joint cartilage.
Mesh models of patellofemoral joint were constructed with CT images and integrated with soft tSayı components such as menisci and ligaments. Physiological and sagittal tilt models during extension and flexion at 15A degrees, 30A degrees and 60A degrees were created generating eight models. All the models were applied with 137 N force to present the effects of normal loading and 115.7 N force for the simulation of quadriceps atrophy. Different degrees of loading were applied to evaluate the joint contact area and pressure value with the finite element analysis.
There was increased patellofemoral contact area in patellar tilt models with respect to normal models. The similar loading patterns were diagnosed in all models at 0A degrees and 15A degrees knee flexion when 137 N force was applied. Higher loading values were obtained at 30A degrees and 60A degrees knee flexions in sagittal tilt models. Furthermore, in the sagittal tilt models, in which the quadriceps atrophy was simulated, the loadings at 30A degrees and 60A degrees knee flexion were higher than in the physiological ones.
Sagittal malalignment of the patellofemoral joint is a new concept that results in different loading patterns in the patellofemoral joint biomechanics. This malalignment in sagittal plane leads to increased loading values on the patellofemoral joint at 30A degrees and 60A degrees of the knee flexions. This new concept should be kept in mind during the course of diagnosis and treatment in patients with anterior knee pain. Definition of the exact biomechanical effects of the sagittal tilting will lead to the development of new treatment modalities.
The role of miR-145 in stem cell characteristics of human laryngeal squamous cell carcinoma Hep-2 cells
Karatas, OF (Karatas, Omer Faruk); Suer, I (Suer, Ilknur); Yuceturk, B (Yuceturk, Betul); Yilmaz, M (Yilmaz, Mehmet); Hajiyev, Y (Hajiyev, Yusif); Creighton, CJ (Creighton, Chad J.); Ittmann, M (Ittmann, Michael); Ozen, M (Ozen, Mustafa)
TUMOR BIOLOGY, 37:3, 4183-4192, DOI: 10.1007/s13277-015-4219-z, 2016
Özet için tıklayınızThe cancer stem-like cells (CSLCs) are tumorigenic cells promoting initiation, progression, and spread of the tumor. Accumulating evidences suggested the presence of CSLCs in distinct tumors including laryngeal squamous cell carcinoma (LSCC). MicroRNAs have been proposed as significant regulators of carcinogenesis, and several of them have been demonstrated to have direct roles in survival of CSLCs. In this study, we aimed to explore the role of miR-145, which is downregulated in LSCC, on cancer stem cell potency of laryngeal cancer cells. We initially showed the downregulation of miR-145 expression in tumor tSayı samples and in CD133-enriched CSLCs. Quantitative reverse-transcription PCR (qRT-PCR) analysis of miR-145-transfected Hep-2 cells demonstrated the inhibitory role of miR-145 on stem cell markers like SOX2, OCT4, KLF4, and ABCG2. We, then, investigated the stem cell features of miR-145-overexpressing Hep-2 cells by sphere formation assay, single-cell cloning assay, and aldehyde dehydrogenase (ALDH) assay, which all demonstrated the inhibition of stem cell potency upon miR-145 overexpression. Further qRT-PCR analysis demonstrated altered expression of epithelial to mesenchymal transition markers in miR-145-overexpressing Hep-2 cells. In conclusion, we demonstrated the regulatory role of miR-145 in stem cell characteristics of Hep-2 cells. Based on these results, we propose that miR-145 might carry crucial roles in LSCC tumorigenesis, prognosis, metastasis, chemoresistance, and recurrence through regulating stem cell properties of tumor cells.
Characterizing the paramagnetic behavior of Cu2+ doped nickel(II) dipicolinato by using theoretical and experimental EPR and UV-vis studies
Yildirim, I (Yildirim, Ilkay); Celik, Y (Celik, Yunus); Karabulut, B (Karabulut, Bunyamin)
PHYSICA B-CONDENSED MATTER, 483, 90-93, DOI: 10.1016/j.physb.2015.12.032, 15 2016
Özet için tıklayınızIn this study, the paramagnetism in bis(hydrogeno pyridine-2,6-dicarboxylato) nickel(II) trihydrate, [Ni(Hdpc)(2)]center dot 3H(2)O, has been investigated after doping the sample with Cu2+ ions. The g and hyperfine parameters were obtained by electron paramagnetic resonance (EPR) experiments performed at ambient temperature. The study shows that Cu2+ ion defects the structure and exists interstitially in the lattice having a distorted local environment It also shows the existence of two magnetically inequivalent Cu2+ sites. Experimental values for both EPR and optical spectrum studies were verified by using the appropriate theoretical approaches. (C) 2015 Elsevier B.V. All rights reserved.
In-vitro bond strengths and clinical failure rates of metal brackets bonded with different light-emitting diode units and curing times
Oz, AA (Oz, Abdullah Alper); Oz, AZ (Oz, Aslihan Zeynep); Arici, S (Arici, Selim)
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS, 149:2, 212-216, DOI: 10.1016/j.ajodo.2015.07.036, 2016
Özet için tıklayınızIntroduction: The purpose of this study was to compare the clinical failure rates and the in-vitro bond strengths of metal brackets bonded with different light-emitting diode (LED) devices and curing times. Methods: Forty patients were included in the clinical part of this study. A split-mouth design was used, with the adhesive in group 1 cured for 10 seconds with an LED unit (Elipar S10; 3M Unitek, Monrovia, Calif), and the adhesive in group 2 cured for 3 seconds with another LED unit (VALO Ortho; Ultradent Products, South Jordan, Utah). Bond failures during 12 months of orthodontic treatment were ed. In-vitro performance of the brackets was also compared by bonding brackets to extracted premolars and using the same light units and curing times (n = 20 for each group). The adhesive remnant index was used to determine the bond failure interface. Results: Clinical bond failure rates were 2.90% for the Elipar and 3.16% for the VALO curing units. The difference in bracket failure rates between the 2 LED devices was not statistically significant. No statistically significant difference was found between the in-vitro bond strengths of the groups. Conclusions: Our findings regarding long-term clinical survival rates and in-vitro bond strengths indicate that bracket bonding can be safely accomplished in 10 seconds of light-curing with an Elipar LED and 3 seconds of light-curing with a VALO LED.
Role of miR-145 in human laryngeal squamous cell carcinoma
Karatas, OF (Karatas, Omer Faruk); Yuceturk, B (Yuceturk, Betul); Suer, I (Suer, Ilknur); Yilmaz, M (Yilmaz, Mehmet); Cansiz, H (Cansiz, Harun); Solak, M (Solak, Mustafa); Ittmann, M (Ittmann, Michael); Ozen, M (Ozen, Mustafa)
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 38:2, 260-266, DOI: 10.1002/hed.23890, 2016
Özet için tıklayınızBackgroundLaryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. Incidences of laryngeal SCC have been reported to increase recently. In this study, we aimed to explore the biological effects of miR-145 on laryngeal cancer cells.
MethodsThe relative miR-145 level in laryngeal SCC tumor tSayıs and normal samples was investigated. Then, Hep-2 cells were utilized for functional analysis of miR-145. The proliferation abilities of transfected cells were measured using MTS assay. Scratch assay and single colony migration assay were performed to observe the alterations in migration behavior of transfected cells. Caspase assay and cell cycle analysis were used to investigate the underlying reasons of proliferative inhibition in cells in which miR-145 is overexpressed. Moreover, expression of SOX2 was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis in Hep-2 cells upon miR-145 transfection and its expression was evaluated in tumor and normal tSayı sample of the larynx.
ResultsThe miR-145 expression in laryngeal SCC tumor samples has been shown to be downregulated. The miR-145 overexpression caused inhibition of proliferation and migration in Hep-2 cells through induction of apoptosis and cell cycle arrest. The SOX2 level was demonstrated to be overexpressed in tumor samples and its expression was significantly decreased in miR-145 overexpressed Hep-2 cells.
ConclusionWe have demonstrated the deregulation of miR-145 and SOX2 in laryngeal SCC. Based on these results, we propose that miR-145, as an important regulator of SOX2, carries crucial roles in laryngeal SCC tumorigenesis. (c) 2015 Wiley Periodicals, Inc. Head Neck 38: 260-266, 2016
Revealing the function of a novel splice-site mutation of CHD7 in CHARGE syndrome
Lee, B (Lee, Byeonghyeon); Duz, MB (Duz, Mehmet Bugrahan); Sagong, B (Sagong, Borum); Koparir, A (Koparir, Asuman); Lee, KY (Lee, Kyu-Yup); Choi, JY (Choi, Jae Young); Seven, M (Seven, Mehmet); Yuksel, A (Yuksel, Adnan); Kim, UK (Kim, Un-Kyung); Ozen, M (Ozen, Mustafa)
GENE, 576:2, 776-781, DOI: 10.1016/j.gene.2015.11.006 Part: 2, 1 2016
Özet için tıklayınızMost cases of CHARGE syndrome are sporadic and autosomal dominant. CHD7 is a major causative gene of CHARGE syndrome. In this study, we screened CHD7 in two Turkish patients demonstrating symptoms of CHARGE syndrome such as coloboma, heart defect, choanal atresia, retarded growth, genital abnomalities and ear anomalies. Two mutations of CHD7 were identified including a novel splice-site mutation (c.2443-2A>G) and a previously known frameshift mutation (c.2504_2508delATCTT). We performed exon trapping analysis to determine the effect of the c.2443-2A>G mutation at the transcriptional level, and found that it caused a complete skip of exon 7 and splicing at a cryptic splice acceptor site. Our current study is the second study demonstrating an exon 7 deficit in CHD7. Results of previous studies suggest that the c.2443-2A>G mutation affects the formation of nasal tSayıs and the neural retina during early development, resulting in choanal atresia and coloboma, respectively. The findings of the present study will improve our understanding of the genetic causes of CHARGE syndrome. (C) 2015 Elsevier B.V. All rights reserved.
EPR study of Cu2+ ion doped orotato(nicotinamid)cobalt(II) single crystal
Yildirim, I (Yildirim, I.); Karabulut, B (Karabulut, B.); Buyukgungor, O (Buyukgungor, O.)
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 152, 608-613, DOI: 10.1016/j.saa.2015.01.100 Basım Tarihi: JAN 5 2016
Özet için tıklayınızWe have studied the Cu2+ ion doped orotato(nicotinamid)cobalt(II) complex by using EPR spectroscopy and X-ray diffraction. The single crystal is triclinic with the space group P (1) over bar. The unit cell dimensions of the crystal are a = 7.2785(4) angstrom, b = 10.2349(5) angstrom, c = 12.7372(6) angstrom, alpha = 69.297(4)degrees, beta = 74.791(4)degrees and gamma = 76.995(4)degrees, with Z = 2. We analyzed the EPR spectra of both single crystal and powder of the complex at room temperature. EPR analysis indicates the presence of only one Cu2+ site. We obtained the spin Hamiltonian parameters from the single crystal data for the complex. The spin Hamiltonian parameters are g(x) = 2.032, g(y) = 2.116, g(z) = 2.319, A(x) = 28 G, A(y) = 66 G, A(z) = 126G. These data indicate that the symmetry of paramagnetic center is rhombic. We constructed the ground state wave function of the Cu2+ ion. (C) 2015 Elsevier B.V. All rights reserved.
A Comparison of Mother-Child Interactions of Children with Autism Spectrum Disorders and Typically Developing Children
Dogan, Y (Dogan, Yasemin); Ozdemir, S (Ozdemir, Selda); Selimoglu, OG (Selimoglu, Omur Gurel); Toret, G (Toret, Gokhan); Ozkubat, U (Ozkubat, Ufuk); Duman, ATC (Duman, Ayse Tuba Ceyhun)
ANKARA UNIVERSITESI EGITIM BILIMLERI FAKULTESI OZEL EGITIM DERGISI-ANKARA UNIVERSITY FACULTY OF EDUCATIONAL SCIENCES JOURNAL OF SPECIAL EDUCATION, 17:1, 79-96, DOI:10.1501/Ozlegt_0000000240 Basım Tarihi: 2016
Özet için tıklayınızThis study compares the interactional behaviors of mothers of children with autism spectrum disorders (n=34) and mothers of typically developing children (n=28) through mother-child dyads. Participating mothers and their children were ed while playing in an unstructured environment. Data were analyzed using five distinct mother interactional behavior categories and mother reinforcements used during the interactions. Mother interactional behaviors were: Directiveness, achievement orientation, responsivity, warmth and un-engagement. Study results were discussed in regard to the interactional behaviors of mothers of children with ASD and TD with their children and suggestions for future research were provided.
Obesity and Lower Urinary Tract Symptoms in Women
Bilge, C (Bilge, Cigdem); Kizilkaya Beji, N (Kizilkaya Beji, Nezihe)
FLORENCE NIGHTINGALE JOURNAL OF NURSING-FLORENCE NIGHTINGALE HEMSIRELIK DERGISI, 24:2, 72-79 Basım Tarihi: 2016
Özet için tıklayınızAim: The aim of this study is to determine the relationship between urinary complaints and obesity in women.
Method: This study conducted between December 2008 and December 2009 at the Istanbul University, Istanbul Faculty of Medicine Department of Obstetrics and Gynecology Outpatient Clinic of Gynecology and Urogynecology. Totaly 320 non-pregnant and over 20 (age) women participated in this study. Obesity degree was evaluated by body mass index (BMI). As data collection device The Urinary Symptom Profile-USP and query form (in order to determinate socio-demographic positions of participants) were used. Validity and reliability studies of USP (to Turkish) were done.
Results: It was determined that BMI of 40.3% women was in normal limit, 35.6% lightly overwieght and 24.1 % was obese and over obese. USP total points of normal weigth women was found 2.98 +/- 4.23, lightly overwiegth women's found 16.56 +/- 4.27, obese and over obese' found 24.18 +/- 4.41. According to USP total points when the difference between lower point and total point was statisticaly examined, a significant diff erence between total and lower point was determined (p<0.05). When women urinary complaints investigated type of stress urinary incontinence score was found 4.20 +/- 2.88, overactive bladder score was found 7.54 +/- 5.74 and urinary flow rate score was found 1.18 +/- 1.75. 19.1% of the women complained of urgency and frequency are available 18.8%. Multiple regression analysis model was used to determine the level of influence of the factors affecting the total factor points of USP. 20 patients for USP test-retest reliability were administered again two weeks later. Test-retest reliability at a high level has been found that the correlation between any two applications (r=0.93).
Conclusion: Body mass index increases urinary complaints increase too.
The altered promoter methylation of oxytocin receptor gene in autism
Yuksel, ME (Yuksel, Mine Elagoz); Yuceturk, B (Yuceturk, Betul); Karatas, OF (Karatas, Omer Faruk); Ozen, M (Ozen, Mustafa); Dogangung, B (Dogangung, Burak)
JOURNAL OF NEUROGENETICS, 30:3-4, 280-284, DOI: 10.1080/01677063.2016.1202951 Basım Tarihi: 2016
Özet için tıklayınızAutism spectrum disorder (ASD) is one of the lifelong existing disorders. Abnormal methylation status of gene promoters of oxytonergic system has been implicated as among the etiologic factors of ASDs. We, therefore, investigated the methylation frequency of oxytocin receptor gene (OXTR) promoter from peripheral blood samples of children with autistic features. Our sample includes 66 children in total (22-94 months); 27 children with ASDs according to the DSM-IV-TR and the Childhood Autism Rating Scale (CARS) and 39 children who do not have any autistic like symptoms as the healthy control group. We investigated the DNA methylation status of OXTR promoter by methylation specific enzymatic digestion of genomic DNA and polymerase chain reaction. A significant relationship has been found between ASDs and healthy controls for the reduction of methylation frequency of the regions MT1 and MT3 of OXTR. We could not find any association in the methylation frequency of MT2 and MT4 regions of OXTR. Although our findings indicate high frequency of OXTR promoter hypomethylation in ASDs, there is need for independent replication of the results for a bigger sample set. We expect that future studies with the inclusion of larger, more homogeneous samples will attempt to disentangle the causes of ASDs.
The anti-inflammatory and antioxidant effects of thymoquinone on ceruleine-induced acute pancreatitis in rats
Dur, A (Dur, A.); Kose, H (Kose, H.); Kocyigit, A (Kocyigit, A.); Kocaman, O (Kocaman, O.); Ismayilova, M (Ismayilova, M.); Sonmez, FC (Sonmez, F. C.)
BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY, 117:10, 614-618, DOI: 10.4149/BLL_2016_119 Basım Tarihi: 2016
Özet için tıklayınızINTRODUCTION: The aim of this study is to investigate the potential antioxidant and anti-inflammatory effects of thymoquinone (TQ) on ceruleine-induced acute pancreatitis.
MATERIAL AND METHODS: A total of 14 male Wistar albino rats were divided into 2 groups as follows: (1) normal saline-treated group and (2) thymoquinone- treated groups. For achieving acute pancreatitis, intraperitoneal (IP) cerulein, a stable cholecystokinin (CCK) analogue, was applied in a 50 mcg/kg dose 2 times in one hour interval in total. One hour after last ceruleine injection, IP 2 ml/kg isotonic saline solution was applied to the saline group and IP 5 mg/kg TQ was applied. The rats were sacrificed by decapitation 12 h after the last injection of last medication. Blood samples were taken, and serum interleukin-1 beta (IL-1 beta), amylase, lipase pancreatic, total antioxidant capacity (TAC), total oxidant status (TOS), and pancreatic Schoenberg scores were determined. Oxidative stress index (OSI) was calculated for each group. Results are given as mean SD. A value of p < 0.05 was accepted as statistically significant. SPSS for Windows v15.0 was used for statistical analyses.
RESULTS: The increased serum amylase, lipase levels and histopathological scoring of pancreatic tSayı showed that acute pancreatitis was present in both groups. Furthermore, serum IL-1 beta level was significantly reduced in TQ-administered group (p < 0.05). Although serum TAC level was high and TOS level was low, those changes were not statistically significant. Nevertheless, OSI index, which was driven from TOS/TAC, was significantly low in TQ groups (p < 0.05). Although TQ partially ameliorated the acute pancreatitis in terms of histopathological evaluations, the main effect of it was brought about by reducing the hemorrhage in acute pancreatitis (p < 0.05).
CONCLUSION: In this study, it was shown that TQ can reduce the inflammation and has a positive effect on the oxidative status of organism in inflammatory cases such as acute pancreatitis. This is consistent with partial amelioration of acute pancreatitis in rats given TQ (Tab. 2, Fig. 4, Ref. 31). Text in PDF www.elis.sk.
Posterior interosseous nerve palsy associated with neglected pediatric Monteggia fracture-dislocation: A case report
Demirel, M (Demirel, Mehmet); Saglam, Y (Saglam, Yavuz); Tunali, O (Tunali, Onur)
INTERNATIONAL JOURNAL OF SURGERY CASE REPORTS, 27, 102-106, DOI: 10.1016/j.ijscr.2016.08.011 Basım Tarihi: 2016
Özet için tıklayınızINTRODUCTION: The main goal of the treatment is the anatomical reduction of the ulna fracture and the radial head dislocation in acute and chronic Monteggia cases. Acute pediatric Monteggia lesions are generally treated non-surgically; however, the treatment of chronic Monteggia is challenging. The aim of this article is to share our experiences about treatment of neglected Monteggia lesion.
PRESENTATION OF CASE: A 6-year-old girl who underwent a surgery in our clinic for a missed Bado type-III Monteggia fracture-dislocation of the right elbow with concomitant posterior interosseous nerve (PIN) palsy, which resolved spontaneously after the operation. The operation consisted of open reduction of the radial head, transverse ulnar osteotomy and fixation with an intramedullary Kirchner wire, and annular ligament repair without exploring PIN. The patient was seen in routine follow-up periods until the postoperative first year using plain radiographies. At 16th week follow-up, all functions of the PIN were returned. At first-year follow-up, full range of elbow motion was observed; plain radiographies showed radiocapitellar joint congruency, and Mayo Elbow Performance Index was one hundred.
DISCUSSION: Treatment planning for chronic, neglected or missed Monteggia fractures is challenging. There is no consensus about the definitive treatment in the literature.
CONCLUSION: We recommend anatomic and stable restoration of radiocapitellar joint by correcting ulna deformity. Radiocapitellar fixation and PIN exploration may not be necessary in all neglected Monteggia lesions. (C) 2016 The Yazar(lar). Basım Tarihi by Elsevier Ltd on behalf of IJS Publishing Group Ltd.
Design Strategies, Structures and Molecular Interactions of Small Molecule Src Inhibitors
Olgen, S (Olgen, Sureyya)
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 16:8, 992-1002, DOI: 10.2174/1871520616666160223111800 Basım Tarihi: 2016
Özet için tıklayınızIn recent years, several small molecules approved by FDA for clinical studies are promising anti-cancer agent. Among the kinases, Abelson Leukaemia (Abl), sarcoma (Src), epidermal growth factor receptor (EGFR) and vascular endotelhial growth factor receptor (VEGFR) are considered as primary molecular targets for selective inhibition and the best successful targeted therapy of tyrosine kinase inhibitors (TKIs) has been achieved in the treatment of Bcr (break point cluster)-Abl leukemia.
The majority of type 1 kinase inhibitors target the active conformation of ATP binding site. In consequence of intensive studies on kinases, type 2, type 3 (allosteric) and type 4 (covalent) inhibitors have been discovered beyond the type 1 inhibitors. Although the selectivity is a major problem for type 1 inhibitors, these new type of inhibitors are promising for finding new selective compounds, which may provide other therapeutic options for cancer therapy. They may also be a solution to overcome drug resistance that remains unresolved yet. Three-dimensional structural determination provides the development of specific and highly binding properties of compounds. Studying the prediction of a binding mode of inhibitors, homology model developments from kinase-ligand co-crystal structures and isosteric replacements have been used to improve binding properties of inhibitors. In this review, critical results related to the design strategies of kinase specifically targeted to Src and Bcr-Abl kinases and therapeutic potential of novel inhibitors will be evaluated. The readers will be endowed with the functional role of Src and Bcr-Abl kinases that lead inhibitor design, the structural analysis of binding modes of kinase inhibitors, the current progress in terms of therapeutic interventions and the mission of leading groups in the field.
Higher minor hemoglobin A2 levels in multiple sclerosis patients correlate with lesser disease severity
Ozcan, ME (Ozcan, Muhammed Emin); Ince, B (Ince, Bahri); Karadeli, HH (Karadeli, Hasan Huseyin); Gedikbasi, A (Gedikbasi, Asuman); Asil, T (Asil, Talip); Altinoz, MA (Altinoz, Meric A.)
NEUROPSYCHIATRIC DISEASE AND TREATMENT, 12, 2033-2038, DOI: 10.2147/NDT.S109954 Basım Tarihi: 2016
Özet için tıklayınızObjective: To define whether minor adult hemoglobin A2 (HbA2, alpha 2 delta 2) exerts any protective activity in multiple sclerosis (MS).
Methods: HbA2 levels were measured in 146 MS patients with high performance liquid chromatography and association with MS Severity Scores (MSSS) were determined. HbA2 associations with blood count parameters were also studied using blood counts evaluated on the same day of high performance liquid chromatography sampling. Routine biochemical parameters were also determined to rule out elusively influential factors, such as anemia and thyroid disorders.
Results: HbA2 levels negatively correlated with MSSS (Spearman correlation, R: -0.186, P=0.025). Exclusion of confounding factors with a generalized linear model revealed an even stronger negative correlation between HbA2 and MSSS (P < 0.001). HbA2 positively correlated with red blood cells (RBCs) (R=0.350, P < 0.001) and in turn, RBCs negatively correlated with MSSS (R=-0.180, P=0.031). Average HbA2 levels were highest among patients treated with interferon beta 1 alpha.
Conclusion: RBC fragility is increased in MS, and recent data suggest that circulating free Hb contributes to neural injury in MS. HbA2 and its oxidative denaturation product hemichrome A2 enhance RBC membrane stability to a greater extent than do major HbA or hemichrome A. Reductions in ischemic cerebrovascular vascular events are reported in beta-thalassemia carriers and HbA2 levels are considerably higher in this population. Episodic declines of cerebral blood flow were shown in bipolar disorder, and we have recently shown a protective role of HbA2 against postpartum episodes in females with bipolar disorder. HbA2's erythroprotective functions may reduce free Hb and long-term neural injury in MS.
Efficacy of uncommon surface treatment methods on titanium in order to improve bond strengths for adhesive cementation
Kilicarslan, MA (Kilicarslan, Mehmet Ali); Ozkan, P (Ozkan, Pelin); Mumcu, E (Mumcu, Emre); Deniz, ST (Deniz, Sule Tugba)
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, 30:21, 2345-2356, DOI: 10.1080/01694243.2016.1182825 Basım Tarihi: 2016
Özet için tıklayınızThe aim of this study is to comparison of effects of uncommon surface treatments, especially new alternatives on the adhesive strength between resin and titanium surfaces. Fifty-five titanium disks were prepared and they were separated into 5 groups as follows:(1) Control group; (2) Tribochemical treatment group in the laboratory; (3) Tribochemical treatment group in the clinic; (4) Acid etch group; and (5) Nd:YAG Laser-irradiated group. Surface roughness of the specimens was measured using a profilometer, and the topographic patterns were observed by scanning electron microscope. After these tests, resin cement was applied to the titanium samples. Shear bond test was performed via a universal testing machine at a crosshead speed of 0.5 mm/minute. In addition, the correlation between the surface roughness and bond strength was checked using Spearman correlation test (0.01 level). The highest surface roughness value was observed for the acid etch group (1.53 mu m). The highest mean shear bond strength was ed with the tribochemical procedure group in the laboratory (13.74 MPa) and the lowest with the control group (3.69 MPa). A positive correlation was found between the bond strength and surface roughness for all groups. All of the surface treatment methods that were used in present study increased the bond strength between resin and titanium except for the laser group.
Nanoleakage within adhesive-dentin interfaces made with simplified ethanol-wet bonding
Ayar, MK (Ayar, Muhammet Kerim); Yildirim, T (Yildirim, Tahsin); Yesilyurt, C (Yesilyurt, Cemal)
JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, 30:22, 2511-2521, DOI: 10.1080/01694243.2016.1185824 Basım Tarihi: 2016
Özet için tıklayınızObjective: The aim of the present study was to evaluate the effects of the simplified ethanol-wet bonding technique and conventional water-wet bonding technique on short term nanoleakage within hybrid layers made with two different etch-and-rinse adhesives (Single Bond 2 and Prime & Bond NT). Materials and methods: Flat dentin surfaces from bovine incisors were prepared and finished with wet 600-grit abrasive papers, then divided into groups to be bonded with one of the adhesives. After etching and rinsing, dentin surfaces were either moistened with water (water-wet bonding technique) or moistened with absolute ethanol for 1 min (ethanol-wet bonding technique). Then, adhesives were applied, and composite buildups were done. Bonded teeth samples were sectioned into resin-dentin samples by means of a low speed diamond saw under water cooling. Specimens were immersed into a 50% (w/v) solution of silver ammoniacal nitrate for 18 h and exposed to photodeveloping solution for 6 h. The amount of silver nitrate uptake within the adhesive layer, or hybrid layer, was measured with energy dispersive spectroscopy (EDS) in different regions (n = 30). Results: Simplified ethanol-wet bonding significantly reduced nanoleakage within resin-dentin interfaces made with Single Bond 2 and Prime & Bond NT, but improvement at the nanoleakage of Single Bond 2 was only significant. Conclusion: Simplified ethanol-wet bonding may improve quality of hybrid layers made with commercially available simplified etch-and-rinse adhesives. But benefits of ethanol-wet bonding may depend on product.
Diagnostic Value of Upar, IL-33, and ST2 Levels in Childhood Sepsis
Cekmez, F (Cekmez, Ferhat); Fidanci, MK (Fidanci, M. Kursat); Ayar, G (Ayar, Ganime); Saldir, M (Saldir, Mehmet); Karaoglu, A (Karaoglu, Abdulbaki); Gunduz, RC (Gunduz, Ramiz C.); Tunc, T (Tunc, Turan); Kalkan, G (Kalkan, Gokhan)
CLINICAL LABORATORY, 62:5, 751-755, DOI: 10.7754/Clin.Lab.2014.141013 Basım Tarihi: 2016
Özet için tıklayınızBackground: The aim of this study was to evaluate the diagnostic value of Upar, IL-33, and ST2 in comparison with C-reactive protein, TNF-alpha, and Interleukin-6 in childhood sepsis.
Methods: A total of 128 children were included and 20 of them were the control group. We used only data showing a high probability of sepsis with blood culture positive children, because of this reason 68 children were excluded. Blood was collected from children from first day of sepsis (1st value) and 48 - 72 hours later (2nd value).
Results: There were significant differences between control and sepsis (1st value) for IL-33 levels (1.1 +/- 0.28 ng/mL and 5.23 +/- 1.80 ng/mL, p = 0.01), for sST2 levels (6.73 +/- 5.3 ng/mL and 53.23 +/- 28.30 ng/mL, p = 0.01), for sUpar levels (3.3 +/- 1.7 ng/mL and 15.2 +/- 6.3 ng/mL, p = 0.01), respectively. There were significant differences between sepsis (1st value) and sepsis (2nd value) for IL-33 levels, for sST2 levels, and for suPAR levels.
Conclusions: In the light of these results, it may be suggested that Upar, IL-33, and ST2 can be used as an acute phase reactant like C-reactive protein, TNF-alpha, and Interleukin-6 in the diagnosis of childhood sepsis.
Developing a Model to Explain Perceptions of Parental Involvement to Education
Kaya, O (Kaya, Ozer); Bacanli, H (Bacanli, Hasan)
HACETTEPE UNIVERSITESI EGITIM FAKULTESI DERGISI-HACETTEPE UNIVERSITY JOURNAL OF EDUCATION, 31:2, 410-423 Basım Tarihi: 2016
Özet için tıklayınızThe overall goal of the study is to establish a model in which the relationship between parental involvement and motivational factors, parents' perceptions of invitations and perceived time and energy. The instruments used in the study which was a causal comparison model were Parent Involvement Questionnaire, Parental Role Construction Scale, School Experience Scale, Parental Efficacy Scale, Parents' Perceptions of Invitations for Involvement from the Others and Perceived Time and Energy Scale. The subjects of the research were composed of 790 parents (418 mothers, 372 fathers). The data were analyzed via SPSS 16.0 and AMOS 7.0 software. According to research findings, parents' perceptions of invitations for involvement from the others directly affected parental involvement. Meanwhile, it was found that parents' perceptions of invitations for involvement from the others and parental involvement had a positive relationship. Perceived time and energy directly affected parental involvement. Besides, it was found that perceived time and energy and parental involvement had also a positive relationship. Parents' perceptions of invitations for involvement from the others had a high level of effect and perceived time and energy had a low level of effect on parental involvement. Parents' motivational factors indirectly affected parental involvement. Meanwhile, they had a positive relationship.
A Study on the Development of a Mathematics Creativity Scale
Akgul, S (Akgul, Savas); Kahveci, NG (Kahveci, Nihat Gurel)
EURASIAN JOURNAL OF EDUCATIONAL RESEARCH:62, 57-76 Basım Tarihi: 2016
Özet için tıklayınızStatement of Problem: Although there is a rapidly increasing interest in creativity in specific domains such as science and math, there are not enough valid and reliable scales to assess students' creativity in these domains. If mathematical creativity potential can be measured, then the curriculum can be adapted to address the needs of creative students. In addition to measuring mathematical creativity potential, it is crucial to identify students at as early an age as possible and develop a mathematics curriculum that would complement and supplement their potential (Balka, 1974; Mann, 2005). For these reasons, valid and reliable scales of measurement are necessary for determining the middle school students' (5th, 6th, 7th and 8th grade: 10-15 years old) mathematical creativity.
Purpose of the Study: The purpose of this study is to develop a valid and reliable Mathematical Creativity Scale for middle school students (5th, 6th, 7th and 8th grade: 10-15 years old).
Method: The main aim of the study is to develop a valid and reliable scale of math creativity for use with middle school students. The pilot study's sample consists of 50 middle school students who attend 5th, 6th, 7th and 8th grades. The field study's sample consists of 297 students who attend 5th, 6th, 7th and 8th grades at 4 middle schools in Istanbul. Exploratory factor analysis (EFA) was applied to evaluate the factor structure of the scale. Item analyses were conducted to check on item discrimination, internal consistency, and agreement between scorers, construct-related validity, and face validity.
Findings: Item analysis including the calculation of item discrimination, item total and item remainder values showed that each item was consistent with the entire scale and that distinctive powers of the items were at an acceptable level. To test internal consistency, Cronbach's Alpha coefficient value was found to be .80, which supports scale reliability. Since it has a naturally subjective scoring process, the scale was rated by two scorers, and the same scorer re-rated it at a different time. The correlation showed that the scale has an interrater reliability and intrarater reliability. Test-retest coefficient values showed that the scale measurements are consistent. Content, construct and face validity results are presented as a part of validation works of the scale. Acceptability to students' results are also presented.
Results: The study results showed that this scale was an appropriate instrument to evaluate middle school (5th, 6th, 7th and 8th grade) students' mathematical creativity. This scale can be used for measurement of the students' creativity in mathematics for the purpose of educational interventions and also for the purpose of determining gifted and talented students in mathematics.
Investigation of the effect of titanium alloy surface coating with different techniques on titanium-porcelain bonding
Aslan, MA (Aslan, Muhammed Ali); Ural, C (Ural, Cagri); Arici, S (Arici, Selim)
JOURNAL OF PROSTHETIC DENTISTRY, 115:1, 115-122 Basım Tarihi: JAN 2016
Özet için tıklayınızStatement of Problem. The bond strength of dental porcelain to titanium is inadequate as a clinical alternative to conventional alloys for metal ceramic restorations.
Purpose. The purpose of this in vitro study was to evaluate the effects of coating titanium surface with a micro-arc oxidation (MAO) technique and hydroxyapatite (HA) on the bond strength of porcelain to titanium surfaces.
Material and methods. One hundred twenty machined titanium specimens with a dimension of 25x3x0.5 mm were prepared from grade 5 titanium as the metal substrate. Specimens were divided into 6 groups (n=20) according to the surface treatment used: airborne-particle abraded (control), coated with MAO for 5 minutes, coated with MAO for 15 minutes, coated with MAO for 30 minutes, coated with HA, and combination of MAO and HA. Each group was further divided into 2 subgroups (n=10) according to the type of porcelain used: Noritake Ti-22 porcelain or Vita-Titankeramik porcelain. The bond strength was tested with a universal testing machine at a crosshead speed of 0.5 mm/min. Data were analyzed statistically with 2-way ANOVA and Tukey honest significant differences multiple comparison tests (alpha=.05).
Results. For both porcelain groups, the 30-minute MAO groups showed higher bond strength values than those of the control groups (P<.05). In the Vita Titankeramik porcelain subgroup, the specimens coated with HA- did not show any statistical differences compared with those of the control group (P>.05). Surface roughness was affected significantly (P<.001) by the coating procedure compared to that of the the control group.
Conclusions. Coating with either MAO or HA improved titanium-porcelain adhesion.
EPR study of gamma irradiated DL-methionine sulfone single crystals
Karabulut, B (Karabulut, Bunyamin); Yildirim, I (Yildirim, Ilkay)
JOURNAL OF MOLECULAR STRUCTURE, 1102, 186-189, DOI: 10.1016/j.molstruc.2015.08.063, 15 2015
Özet için tıklayınızElectron paramagnetic resonance (EPR) study of gamma irradiated dl-2-amino-4-(Methylsulfonyl) butyric acid (dl-methionine sulfone, hereafter dl-ABA) single crystals and powder was performed at room temperature. It has been found that this compound indicates the existence of CO2- and NH2 radicals after gamma-irradiation. While g and hyperfine splitting values for the NH2 radical were observed, for the CO2- radical, only the g factor was measured. The EPR spectra have shown that NH2 radical has two groups each having two distinct sites and CO2- radical has one site. The principal g and hyperfine values for all sites were analyzed. (C) 2015 Elsevier B.V. All rights reserved.
Nitric oxide mediated effects of nebivolol in myocardial infarction: the Dergi of nitric oxide
Mercanoglu, G (Mercanoglu, G.); Safran, N (Safran, N.); Ahishali, BB (Ahishali, B. B.); Uzun, H (Uzun, H.); Yalcin, A (Yalcin, A.); Mercanoglu, F (Mercanoglu, F.)
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 19:24, 4872-4889, 2015
Özet için tıklayınızOBJECTIVE: After MI pathological LV remodeling is one of the major causes of death. We previously showed the NO mediated beneficial effects of nebivolol in rat MI model, in this study we aimed to evaluate the NOS related mechanisms in this phenomenon.
MATERIALS AND METHODS: Rats were divided into four groups: sham operated (sham-control), MI-induced (MI-control), immediate nebivolol loaded (MI-neb1), orally nebivolol treated (MI-neb2). MI was induced by the ligation of the LAD. Loading dose of nebivolol (0.1 mg/kg) was administrated i.v. during reperfusion and continuation dose was administrated orally (2 mg/kg) by gastric gavages once daily. NOS related mechanisms were assessed either in acute (2nd day) and sub-acute (28th day) period of MI by histologic, hemodynamic and biologic studies.
RESULTS: Compared to MI-control rats, physiological functions of LV (LVEDP, Delta +/- dp/dt) were prevented in both nebivolol treated groups. Improvements in anatomical parameters (LEV, HW, LVW/HW) were consistent with functional improvement too. Moreover, oxidative (characterized by decreased MDA and increased SOD levels) and nitrosative (characterized by decreased ONOO-levels) damage were limited in these groups. Compared to MI-control rats, most marked change was seen in the nNOS labelling in the nebivolol treated groups. The decrease in iNOS labelling was also prominent in these groups too.
CONCLUSIONS: NOS mediated mechanisms of nebivolol can be summarized as: 1) diminishing iNOS expression together with restoration of MI induced eNOS activation both in vascular bed and myocytes at the acute period of MI, and 2) prevention of deterioration in nNOS expression in myocardial cells at the sub-acute period of MI.
Effectiveness of platelet-rich plasma in the treatment of moderate knee osteoarthritis: a randomized prospective study
Kavadar, G (Kavadar, Gulis); Demircioglu, DT (Demircioglu, Demet Tekdos); Celik, MY (Celik, Memet Yusuf); Emre, TY (Emre, Tuluhan Yunus)
JOURNAL OF PHYSICAL THERAPY SCIENCE, 27:12, 3863-3867, 2015
Özet için tıklayınız[Purpose] To assess the effects of different numbers of platelet-rich plasma (PRP) applications on pain and physical function in grade 3 knee osteoarthritis (OA). [Subjects and Methods] A total of 102 patients with grade 3 knee OA were randomly divided into three groups: Group 1 received a single injection of PRP, Group 2 received two injections of PRP two weeks apart, Group 3 received three injections of PRP at 2-weeks intervals. All patients were evaluated with a visual analog scale (VAS), the Western Ontario and McMaster Universities Arthritis Index (WOMAC), and the Timed-Up and Go test (TUG) before the treatment and at 1, 3 and 6 months after the treatment. [Results] Ninety-eight patients (15 males, 83 females) completed the study. The mean ages of the patients were 53.5 +/- 6.6, 54.9 +/- 5.3, and 55.1 +/- 5.6 years in Group 1, Group 2, and Group 3, respectively. Statistically significant improvements were noted in all of the evaluated measures in all of the groups. The mean differences of Group 1-Group 2 and Group 1-Group 3 WOMAC total, WOMAC pain, WOMAC stiffness, and WOMAC function scores were statistically significant. [Conclusion] PRP is an effective treatment for functional status and pain in moderate knee osteoarthritis and a minimum of two injections is appropriate.
Does social support affect development of cognitive dysfunction in individuals with diabetes mellitus?
Yilmaz, FT (Yilmaz, Feride T.); Sabanciogullari, S (Sabanciogullari, Selma); Aldemir, K (Aldemir, Kadriye); Kumsar, AK (Kumsar, Azime K.)
SAUDI MEDICAL JOURNAL, 36:12, 1425-1431, 2015
Özet için tıklayınızObjectives: To determine cognitive functions and perceived social support (SS) among individuals with diabetes mellitus (DM), and the effects of SS on the development of cognitive dysfunction (CD).
Methods: This cross-sectional study was conducted in 121 patients with DM presenting at the Endocrinology Clinic of Cumhuriyet University Health Services Application and Research Hospital, Sivas, Turkey between April and June 2014. Data were collected utilizing the "Patient Assessment Form", "Standardized Mini Mental State Examination (SMMSE)", and "Multidimensional Scale of Perceived Social Support (MSPSS)".
Results: The mean score obtained for DM patients from the SMMSE was 21.55 +/- 5.7, with 65.3% found to have cognitive impairment. The total mean score of the participants for MSPSS was considered moderate (66.61 +/- 14.42). There was a significant positive correlation between cognitive function and SS (r=0.273, p=0.002). It was determined that individuals with CD had low levels of perceived SS, and that insufficient support from families and significant others contributed to the development of CD (p=0.008).
Conclusion: In this study, it was determined that the cognitive function of individuals with DM was impaired and would improve as the perception of SS increased, and that perceived SS would affect the development of CD. Therefore, health professionals can contribute to the improvement of cognitive function of individuals with DM by facilitating the use of SS Dergis.
MiR-221 as a Pre- and Postoperative Plasma Biomarker for Larynx Cancer Patients
Yilmaz, SS (Yilmaz, Seda Salman); Guzel, E (Guzel, Esra); Karatas, OF (Karatas, Omer Faruk); Yilmaz, M (Yilmaz, Mehmet); Creighton, CJ (Creighton, Chad J.); Ozen, M (Ozen, Mustafa)
LARYNGOSCOPE, 125:12, E377-E381, DOI: 10.1002/lary.25332, 2015
Özet için tıklayınızObjective: In order to identify a plasma microRNA (miRNA) signature of larynx cancer (LCa), we examined miRNAs profile of plasma samples obtained from 30 LCa patients (preoperative and postoperative serum samples) and 30 healthy controls.
Study Design: Basic science research study.
Methods: MicroRNA profiling of eight plasma samples (four from preoperative, four from control individuals) were performed using miRNA microarray. Two of the significantly deregulated miRNAs were selected for further confirmation in the remaining samples using quantitative reverse-transcription polymerase chain reaction (qRT-PCR).
Results: Microarray profiling and qRT-PCR analysis showed that miR-221 was upregulated in LCa plasma samples. Further qRT-PCR analysis demonstrated that miR-221 was at normal levels in postoperative plasma samples.
Conclusions: Plasma miR-221 may have a potential as a novel diagnostic/prognostic marker and might be considered as a therapeutic target in LCa.
Gingival Crevicular Fluid Levels of Sclerostin, Osteoprotegerin, and Receptor Activator of Nuclear Factor-kappa B Ligand in Periodontitis
Balli, U (Balli, Umut); Aydogdu, A (Aydogdu, Ahmet); Dede, FO (Dede, Figen Ongoz); Turer, CC (Turer, Cigdem Coskun); Guven, B (Guven, Berrak)
JOURNAL OF PERIODONTOLOGY, 86:12, 1396-1404, DOI: 10.1902/jop.2015.150270, 2015
Özet için tıklayınızBackground: To investigate changes in the levels and relative ratios of sclerostin, osteoprotegerin (OPG), and receptor activator of nuclear factor-kappa B ligand (RANKL) in the gingival crevicular fluid (GCF) of patients with periodontitis after non-surgical periodontal treatment.
Methods: Fifty-four individuals (27 healthy controls and 27 patients with chronic periodontitis [CP]) were enrolled in the study. Periodontitis patients received non-surgical periodontal therapy. GCF sampling and clinical periodontal parameters were assessed before and 6 weeks after therapy. Sclerostin, OPG, and RANKL levels were measured by enzyme-linked immunosorbent assay, and their relative ratios were calculated.
Results: Total amounts and concentrations of sclerostin were significantly higher in patients with CP than in healthy individuals (P < 0.025) and decreased after treatment (P < 0.05). The RANKL/OPG ratio was significantly lower in healthy individuals than in patients with periodontitis before and after treatment (P < 0.025), but no significant difference was observed in patients with periodontitis after treatment (P > 0.05). The sclerostin/OPG and sclerostin/RANKL ratios were significantly lower in healthy individuals than in patients with periodontitis before and after treatment (P < 0.025) and decreased in patients with periodontitis after treatment (P < 0.05).
Conclusions: The GCF sclerostin level may be more reliable than the RANKL/OPG ratio as a diagnostic and prognostic marker of periodontal disease and treatment outcome. Regulation of sclerostin levels may aid the development of new therapeutic strategies for the treatment of periodontal disease.
STAT-dependent upregulation of 12/15-lipoxygenase contributes to neuronal injury after stroke
Jung, JE (Jung, Joo Eun); Karatas, H (Karatas, Hulya); Liu, Y (Liu, Yu); Yalcin, A (Yalcin, Ayfer); Montaner, J (Montaner, Joan); Lo, EH (Lo, Eng H.); van Leyen, K (van Leyen, Klaus)
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 35:12, 2043-2051, DOI: 10.1038/jcbfm.2015.169, 2015
Özet için tıklayınızOxidative stress is a major brain injury mechanism after ischemic stroke. 12/15-lipoxygenase (12/15-LOX) is a key mediator of oxidative stress, contributing to neuronal cell death and vascular leakage. Nonetheless, the mechanism leading to its upregulation is currently unknown. We show here that Signal Transducers and Activators of Transcription (STATs), specifically STAT6 and possibly STAT1, increase transcription of 12/15-LOX in neuronal cells. Both p-STAT6 and -1 bound to specific STAT binding sites in the mouse 12/15-LOX promoter. Small interfering RNA (siRNA) knockdown showed STAT6 to be the dominant regulator, reducing 12/15-LOX promoter activation and cell death in oxidatively stressed HT22 cells. STAT6 siRNA efficiently prevented the increase of 12/15-LOX in murine primary neurons, both after induction of oxidative stress and after oxygen-glucose deprivation. Early activation of STAT6 and STAT1 in mice was consistent with a role in regulating 12/15-LOX in focal ischemia. Brains of human stroke patients showed increased p-STAT6 and p-STAT1 in the peri-infarct region, along with 12/15-LOX and markers of apoptosis. These results link STAT6 and STAT1 to the 12/15-LOX damage pathway and suggest disregulation of STAT-dependent transcription as injury mechanism in stroke. Selectively targeting STATs may thus be a novel therapeutic approach to reducing brain injury after a stroke.
Circulating miR-21 and eNOS in subclinical atherosclerosis in patients with hypertension
Cengiz, M (Cengiz, Mahir); Yavuzer, S (Yavuzer, Serap); Avci, BK (Avci, Burcak Kilickiran); Yuruyen, M (Yuruyen, Mehmet); Yavuzer, H (Yavuzer, Hakan); Dikici, SA (Dikici, Suleyman Atilla); Karatas, OF (Karatas, Omer Faruk); Ozen, M (Ozen, Mustafa); Uzun, H (Uzun, Hafize); Ongen, Z (Ongen, Zeki)
CLINICAL AND EXPERIMENTAL HYPERTENSION, 37:8, 643-649, DOI: 10.3109/10641963.2015.1036064, 17 2015
Özet için tıklayınızObjectives: The aim of this study is to evaluate the relationship of miR-21, nitric oxide (NOx) and endothelial nitric oxide synthase (eNOS) with subclinical atherosclerosis in carotid arteries by measuring carotid intima media thickness (CIMT) in patients with hypertension and healthy controls. Design and Methods: A total of 28 hypertensive and 28 healthy controls were enrolled. MiR-21 expression was analyzed by quantitative reverse transcription-PCR and NOx, and eNOS levels were measured by ELISA assay. CIMT was evaluated by ultrasonography and CIMT0.8mm was accepted as increased CIMT (iCIMT). Results: C-reactive protein (CRP) level, plasma miR-21 expression level and CIMT were found to be significantly higher in the hypertension group when compared to the control group (p=0.009, p=0.002 and p<0.001, respectively). NOx and eNOS levels were significantly lower in the hypertension group compared to the control group (p<0.001, both). MiR-21 level was positively correlated with the clinical systolic blood pressure, clinical diastolic blood pressure, CRP and CIMT. MiR-21 was also negatively correlated with NOx and eNOS. Eighteen patients with hypertension had iCIMT. MiR-21 and CRP levels were significantly higher (p<0.001 and p=0.001), whereas NOx and eNOS levels were significantly lower in patients with iCIMT (p<0.001, both). Conclusion: The decreased levels of NOx and eNOS found in this study indicate the co-existence of endothelial dysfunction and hypertension once more. In the absence of microalbuminuria, the increased miR-21 expression in patients with iCIMT made us conclude that this miRNA might be involved in the early stages of atherosclerotic process in hypertensive patients.
Genes that Affect Brain Structure and Function Identified by Rare Variant Analyses of Mendelian Neurologic Disease
Karaca, E (Karaca, Ender); Harel, T (Harel, Tamar); Pehlivan, D (Pehlivan, Davut); Jhangiani, SN (Jhangiani, Shalini N.); Gambin, T (Gambin, Tomasz); Akdemir, ZC (Akdemir, Zeynep Coban); Gonzaga-Jauregui, C (Gonzaga-Jauregui, Claudia); Erdin, S (Erdin, Serkan); Bayram, Y (Bayram, Yavuz); Campbell, IM (Campbell, Ian M.); Hunter, JV (Hunter, Jill V.); Atik, MM (Atik, Mehmed M.); Van Esch, H (Van Esch, Hilde); Yuan, B (Yuan, Bo); Wiszniewski, W (Wiszniewski, Wojciech); Isikay, S (Isikay, Sedat); Yesil, G (Yesil, Gozde); Yuregir, OO (Yuregir, Ozge O.); Bozdogan, ST (Bozdogan, Sevcan Tug); Aslan, H (Aslan, Huseyin); Aydin, H (Aydin, Hatip); Tos, T (Tos, Tulay); Aksoy, A (Aksoy, Ayse); De Vivo, DC (De Vivo, Darryl C.); Jain, P (Jain, Preti); Geckinli, BB (Geckinli, B. Bilge); Sezer, O (Sezer, Ozlem); Gul, D (Gul, Davut); Durmaz, B (Durmaz, Burak); Cogulu, O (Cogulu, Ozgur); Ozkinay, F (Ozkinay, Ferda); Topcu, V (Topcu, Vehap); Candan, S (Candan, Sukru); Cebi, AH (Cebi, Alper Han); Ikbal, M (Ikbal, Mevlit); Gulec, EY (Gulec, Elif Yilmaz); Gezdirici, A (Gezdirici, Alper); Koparir, E (Koparir, Erkan); Ekici, F (Ekici, Fatma); Coskun, S (Coskun, Salih); Cicek, S (Cicek, Salih); Karaer, K (Karaer, Kadri); Koparir, A (Koparir, Asuman); Duz, MB (Duz, Mehmet Bugrahan); Kirat, E (Kirat, Emre); Fenercioglu, E (Fenercioglu, Elif); Ulucan, H (Ulucan, Hakan); Seven, M (Seven, Mehmet); Guran, T (Guran, Tulay); Elcioglu, N (Elcioglu, Nursel); Yildirim, MS (Yildirim, Mahmut Selman); Aktas, D (Aktas, Dilek); Alikasifoglu, M (Alikasifoglu, Mehmet); Ture, M (Ture, Mehmet); Yakut, T (Yakut, Tahsin); Overton, JD (Overton, John D.); Yuksel, A (Yuksel, Adnan); Ozen, M (Ozen, Mustafa); Muzny, DM (Muzny, Donna M.); Adams, DR (Adams, David R.); Boerwinkle, E (Boerwinkle, Eric); Chung, WK (Chung, Wendy K.); Gibbs, RA (Gibbs, Richard A.); Lupski, JR (Lupski, James R.)
NEURON, 88:3, 499-513, DOI: 10.1016/j.neuron.2015.09.048, 4 2015
Özet için tıklayınızDevelopment of the human nervous system involves complex interactions among fundamental cellular processes and requires a multitude of genes, many of which remain to be associated with human disease. We applied whole exome sequencing to 128 mostly consanguineous families with neurogenetic disorders that often included brain malformations. Rare variant analyses for both single nucleotide variant (SNV) and copy number variant (CNV) alleles allowed for identification of 45 novel variants in 43 known disease genes, 41 candidate genes, and CNVs in 10 families, with an overall potential molecular cause identified in >85% of families studied. Among the candidate genes identified, we found PRUNE, VARS, and DHX37 in multiple families and homozygous loss-of-function variants in AGBL2, SLC18A2, SMARCA1, UBQLN1, and CPLX1. Neuroimaging and in silico analysis of functional and expression proximity between candidate and known disease genes allowed for further understanding of genetic networks underlying specific types of brain malformations.
The effects of very early mirror therapy on functional improvement of the upper extremity in acute stroke patients
Yeldan, I (Yeldan, Ipek); Huseyinsinoglu, BE (Huseyinsinoglu, Burcu Ersoz); Akinci, B (Akinci, Buket); Tarakci, E (Tarakci, Ela); Baybas, S (Baybas, Sevim); Ozdincler, AR (Ozdincler, Arzu Razak)
JOURNAL OF PHYSICAL THERAPY SCIENCE, 27:11, 3519-3524, 2015
Özet için tıklayınız[Purpose] The aim of the study was to evaluate the effects of a very early mirror therapy program on functional improvement of the upper extremity in acute stroke patients. [Subjects] Eight stroke patients who were treated in an acute neurology unit were included in the study. [Methods] The patients were assigned alternatively to either the mirror therapy group receiving mirror therapy and neurodevelopmental treatment or the neurodevelopmental treatment only group. The primary outcome measures were the upper extremity motor subscale of the Fugl-Meyer Assessment, Motricity Index upper extremity score, and the Stroke Upper Limb Capacity Scale. Somatosensory assessment with the Ayres Southern California Sensory Integration Test, and the Barthel Index were used as secondary outcome measures. [Results] No statistically significant improvements were found for any measures in either group after the treatment. In terms of minimally clinically important differences, there were improvements in Fugl-Meyer Assessment and Barthel Index in both mirror therapy and neurodevelopmental treatment groups. [Conclusion] The results of this pilot study revealed that very early mirror therapy has no additional effect on functional improvement of upper extremity function in acute stroke patients. Multicenter trials are needed to determine the results of early application of mirror therapy in stroke rehabilitation.
Is plantar foot sensation associated with standing balance in type 2 diabetes mellitus patients
Kafa, N (Kafa, Nihan); Citaker, S (Citaker, Seyit); Tuna, Z (Tuna, Zeynep); Guney, H (Guney, Hande); Kaya, D (Kaya, Defne); Guzel, NA (Guzel, Nevin Atalay); Basar, S (Basar, Selda); Yetkin, I (Yetkin, Ilhan)
INTERNATIONAL JOURNAL OF DIABETES IN DEVELOPING COUNTRIES, 35, S405-S410, DOI: 10.1007/s13410-015-0396-y Supplement: 3, 2015
Özet için tıklayınızTo examine the relationship between one-leg standing balance (OLSB) and different modalities of foot sole sensation in patients with type 2 diabetes mellitus (T2DM). Forty patients diagnosed with T2DM (mean age, 53.96 +/- 15.31 years) and ten healthy control subjects were included. Duration of OLSB was measured with digital chronometer. Threshold of light-touch pressure (LTP), evaluated with full Semmes-Weinstein Monofilament Test Kit, and distance of two-point discrimination (TPD), evaluated with aesthesiometer, were assessed at six different regions of the foot sole. Thresholds of the LTP at all regions were significantly correlated with duration of OLSB (p<0.05). However, there was no relationship between TPD sensation and duration of OLSB (p>0.05). LTP at the plantar side of the great toe was the most related area with the OLSB (r=-0.48, p<0.05). In patients with T2DM, LTP, and TPD sensations of foot sole and duration of OLSB were decreased compared to healthy controls. In this study, there was a moderate correlation between LTP sensation and balance. Other factors that would affect the balance in patients with T2DM should be investigated.
Novel POC1A mutation in primordial dwarfism reveals new insights for centriole biogenesis
Koparir, A (Koparir, Asuman); Karatas, OF (Karatas, Omer F.); Yuceturk, B (Yuceturk, Betul); Yuksel, B (Yuksel, Bayram); Bayrak, AO (Bayrak, Ali O.); Gerdan, OF (Gerdan, Omer F.); Sagiroglu, MS (Sagiroglu, Mahmut S.); Gezdirici, A (Gezdirici, Alper); Kirimtay, K (Kirimtay, Koray); Selcuk, E (Selcuk, Ece); Karabay, A (Karabay, Arzu); Creighton, CJ (Creighton, Chad J.); Yuksel, A (Yuksel, Adnan); Ozen, M (Ozen, Mustafa)
HUMAN MOLECULAR GENETICS, 24:19, 5378-5387, DOI: 10.1093/hmg/ddv261, 1 2015
Özet için tıklayınızPOC1A encodes a WD repeat protein localizing to centrioles and spindle poles and is associated with short stature, onychodysplasia, facial dysmorphismand hypotrichosis (SOFT) syndrome. These main features are related to the defect in cell proliferation of chondrocytes in growth plate. In the current study, we aimed at identifying the molecular basis of two patients with primordial dwarfism (PD) in a single family through utilization of whole-exome sequencing. A novel homozygous p.T120A missense mutation was detected in POC1A in both patients, a known causative gene of SOFT syndrome, and confirmed using Sanger sequencing. To test the pathogenicity of the detected mutation, primary fibroblast cultures obtained from the patients and a control individual were used. For evaluating the global gene expression profile of cells carrying p.T120A mutation in POC1A, we performed the gene expression array and compared their expression profiles to those of control fibroblast cells. The gene expression array analysis showed that 4800 transcript probes were significantly deregulated in cells with p.T120A mutation in comparison to the control. GO term association results showed that deregulated genes are mostly involved in the extracellular matrix and cytoskeleton. Furthermore, the p.T120A missense mutation in POC1A caused the formation of abnormal mitotic spindle structure, including supernumerary centrosomes, and changes in POC1A were accompanied by alterations in another centrosome-associated WD repeat protein p80-katanin. As a result, we identified a novel mutation in POC1A of patients with PD and showed that this mutation causes the formation of multiple numbers of centrioles and multipolar spindles with abnormal chromosome arrangement.
DECISION TREE APPLICATION TO REDUCE INCORRECT DIAGNOSIS IN SYMPTOMS OF JAUNDICE
Topaloglu, M (Topaloglu, Murat); Sur, H (Sur, Haydar)
NOBEL MEDICUS, 11:3, 64-73, 2015
Özet için tıklayınızObjective: In this study, decision support system was created to diagnose jaundice and to reduce incorrect diagnosis of jaundice. The study was conducted to help physicians to diagnose jaundice.
Material and Method: In this study, data of 300 patients diagnosed with jaundice was taken from a health institution. All tests performed for the patients were included in the study from the beginning of complaints to the final diagnosis. To this end, data of patients previously diagnosed with jaundice were collected in the database, edited, and organized. Training kit was created with this organized data. Then data mining methods applied to agreed algorithms and the decision tree models were created from the results. Decision trees were created by using C5.0 and J48 algorithms. The purpose of the decision tree is to reach 16 illness using 21 qualities.
Results: In decision support system are two different decision trees and separate Sayfa designed according to expert opinion. The purpose is not to create decision support system with a single decision tree, but to identify the disease with three different opinions. Trimming operations were carried out in trees created with the algorithms. With most appropriate trimming 16 illnesses were reached with 100% accuracy.
Conclusion: Decision support system helping the physicians be able to use the decision trees in clinic environment easily has been developed. This study was conducted with the aim of preventing time loss in diagnosis phase and helping the physicians diagnose jaundice accurately.
Alpha-B-crystallin expression in human laryngeal squamous cell carcinoma tSayıs
Yilmaz, M (Yilmaz, Mehmet); Karatas, OF (Karatas, Omer Faruk); Yuceturk, B (Yuceturk, Betul); Dag, H (Dag, Huseyin); Yener, M (Yener, Murat); Ozen, M (Ozen, Mustafa)
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, 37:9, 1344-1348, DOI: 10.1002/hed.23746, 2015
Özet için tıklayınızBackgroundLaryngeal squamous cell carcinoma (SCC), being an aggressive malignancy, is one of the most commonly diagnosed malignant types of head and neck SCC worldwide. The recent studies suggested that B-crystallin might play an important role in tumorigenesis. The purpose of this study was to investigate the B-crystallin expression level in metastatic and nonmetastatic laryngeal SCC tSayıs and to determine its prognostic significance.
MethodsAlpha-B-crystallin expression status in metastatic, nonmetastatic laryngeal SCC, and normal tSayı samples was investigated by quantitative real-time polymerase chain reaction (qRT-PCR).
ResultsWe demonstrated that the expression of B-crystallin was significantly upregulated in laryngeal SCC tumor tSayı samples in comparison with the corresponding normal tSayıs (p<.001), although no significant association has been found between B-crystallin expression and either the metastatic potential or the T classification of the specimens.
ConclusionAlthough expression of B-crystallin is not statistically correlated with neck metastases, we demonstrated that B-crystallin is significantly overexpressed in laryngeal cancer. (c) 2014 Wiley Periodicals, Inc. Head Neck 37: 1344-1348, 2015
A computational insight into acetylcholinesterase inhibitory activity of a new lichen depsidone
Ece, A (Ece, Abdulilah); Pejin, B (Pejin, Boris)
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 30:4, 528-532, DOI: 10.3109/14756366.2014.949256, 2015
Özet için tıklayınızAcetylcholinesterase (AChE) inhibitors are yet the best drugs currently available for the management of Alzheimer's disease. The recent phytochemical investigation has led to the isolation of a new depsidone 1 with moderate AChE activity (1 mu g). This work was focused on its electronic properties analysed using commercially available programs. Both the active depsidone molecule 1 and galanthamine showed to have higher HOMO energies than the inactive depsidones 2-4, isolated from the same lichen species. However, the amino depsidone derivative 7, whose structure was proposed using computational approaches, is expected to be more active AChE inhibitor than the depsidone 1, due to the improved HOMO energy value. In addition, the molecular docking study indicated that the compound 7 has ability to make the well-known interactions of potent AChE inhibitors with the enzyme active site. The data presented herein support the design of novel AChE inhibitors based on the depsidone scaffold.
Renal biopsy in the elderly: a single-center experience
Harmankaya, O (Harmankaya, Ozlem); Okuturlar, Y (Okuturlar, Yildiz); Kocoglu, H (Kocoglu, Hakan); Kaptanogullari, H (Kaptanogullari, Hakan); Yucel, SK (Yucel, Sibel Kocak); Ozkan, H (Ozkan, Hanise); Acarer, D (Acarer, Didem); Erdogan, E (Erdogan, Ezgi); Yilmaz, M (Yilmaz, Murvet); Hursitoglu, M (Hursitoglu, Mehmet)
INTERNATIONAL UROLOGY AND NEPHROLOGY, 47:8, 1397-1401, DOI: 10.1007/s11255-015-1035-8, 2015
Özet için tıklayınızAging population has been increasing worldwide. So the number of elderly patients presenting with kidney disease has also been increasing. In this retrospective study, we assessed biopsy results of elderly patients and their clinical presentations.
Native renal biopsy results of 98 elderly patients (a parts per thousand yen65 years) were analyzed. These 98 patients consisted of 56 males (57.1 %) and 42 females (42.9 %) with a mean age of 70.59 +/- A 5.31 years (65-88 years).
The clinical presentations of our elderly patients were nephritic syndrome (n = 45), acute kidney injury (n = 35), nephrotic syndrome (n = 33), chronic kidney disease (n = 32) and combined nephritic and nephrotic syndrome (n = 14). In patients with nephritic syndrome, the most common diagnosis was crescentic GN (17.8 % type 3 and 13.3 % types 1 and 2). Crescentic GN was also the most frequent among patients with acute kidney injury (37.1 %), while membranous nephropathy was the major histopathological diagnosis in chronic kidney disease patients. In nephrotic syndrome, the most common histopathological diagnosis was AA amyloidosis. None of the patients had a major life-threatening complication.
Biopsies in the elderly are as safe as in the general population. Renal biopsy should be performed to provide an accurate diagnosis and initiate specific treatment in elderly patients.
The Place of Green Tea in the Prevention of Osteoporosis in Women
Demirel, G (Demirel, Gulbahtiyar); Kumsar, AK (Kumsar, Azime Karakoc); Yilmaz, FT (Yilmaz, Feride Taskin)
TURK OSTEOPOROZ DERGISI-TURKISH JOURNAL OF OSTEOPOROSIS, 21:2, 84-86, DOI: 10.4274/tod.54376, 2015
Özet için tıklayınızOsteoporosis is one of the most important health-related problems in today's world that it is increasing in incidence specifically after menopause in women. Various risk factors including genetic characteristics, Vitamin D deficiency, insufficient calcium intake, sedentary lifestyle and late menarche enable osteoporosis to be contained in terms of its progress and to be treated. The consumption of green tea is one of the non-pharmacological methods utilized in the prevention of osteoporosis in recent years. Studies conducted abroad with relation to this subject identified that the consumption of green tea is effective in the regulation of bone density and the protection of bone health. However, this finding lacks sufficient data at the national scale. The present review will provide healthcare professionals information on the necessity for the consumption of green tea in the prevention of osteoporosis in women.
Effect of atmospheric plasma versus conventional surface treatments on the adhesion capability between self-adhesive resin cement and titanium surface
Seker, E (Seker, Emre); Kilicarslan, MA (Kilicarslan, Mehmet Ali); Deniz, ST (Deniz, Sule Tugba); Mumcu, E (Mumcu, Emre); Ozkan, P (Ozkan, Pelin)
JOURNAL OF ADVANCED PROSTHODONTICS, 7:3, 249-256, DOI: 10.4047/jap.2015.7.3.249, 2015
Özet için tıklayınızPURPOSE. The aim of this study was to evaluate the effects of atmospheric plasma (APL) versus conventional surface treatments on the adhesion of self-adhesive resin cement to Ti-6Al-4V alloy. MATERIALS AND METHODS. Sixty plates of machined titanium (Ti) discs were divided into five groups (n=12): 1) Untreated (CNT); 2) Sandblasted (SAB); 3) Tribochemically treated (ROC); 4) Tungsten Carbide Bur (TCB); 5) APL treated (APL). SEM analysis and surface roughness (Ra) measurements were performed. Self-adhesive resin cement was bonded to the Ti surfaces and shear bond strength (SBS) tests, Ra and failure mode examinations were carried out. Data were analyzed by one-way analysis of variance and chi-squared test. RESULTS. The lowest SBS value was obtained with CNT and was significantly different from all other groups except for APL. The ROC showed the highest SBS and Ra values of all the groups. CONCLUSION. It was concluded that the effect of APL on SBS and Ra was not sufficient and it may not be a potential for promoting adhesion to titanium.
DNA damage in children with scoliosis following X-ray exposure
Himmetoglu, S (Himmetoglu, S.); Guven, MF (Guven, M. F.); Bilsel, N (Bilsel, N.); Dincer, Y (Dincer, Y.)
MINERVA PEDIATRICA, 67:3, 245-249, 2015
Özet için tıklayınızAim. It has been suggested that cancer incidence is high in subjects with scoliosis who are relatively more often exposed to X-ray for diagnosis and follow-up. X-ray is a kind of ionizing radiation and leads to formation of oxygen free radicals which are capable of damage to DNA, thus altered gen expression and mutation. p53 tumor suppressor gene plays a crucial role in the damage response. It controls the checkpoint of cell cycle and redirects the cell metabolism to either repair of damaged DNA or apoptosis as response to DNA damage. The aim of the present study was to examine serum levels of 8-Hydroxydeoxyguanosine (8-OHdG), a strongly mutagenic product of oxidative DNA damage, p53, superoxide dismutase (SOD) and glutathione peroxidase (G-Px), as antioxidant activity, in children with scoliosis who had got whole spine radiograph two times during the last year.
Methods. A total of 31 children with adolescent idiopathic scoliosis and 21 age-matched healthy children were included in the study. Serum levels of 8-OHdG and p53 were measured with ELISA kits. SOD and G-Px activities were determined with spectrophotometric assays.
Results. Serum levels of 8-OHdG and p53 were found to be higher (P<0.001 and P<0.01, respectively), SOD activity was found to be lower (P<0.001) in the children with scoliosis as compared to age-matched controls. There was no significant difference between the groups for G-Px activity.
Conclusion. Our data show that X-ray exposure causes increased 8-OHdG level, and decreased SOD activity, which both may reflect a tumor promoting condition. Increased p53 level may be interpreted as a compensatory effort of cell to X-ray mediated DNA damage.
Whole-exome sequencing revealed two novel mutations in Usher syndrome
Koparir, A (Koparir, Asuman); Karatas, OF (Karatas, Omer Faruk); Atayoglu, AT (Atayoglu, Ali Timucin); Yuksel, B (Yuksel, Bayram); Sagiroglu, MS (Sagiroglu, Mahmut Samil); Seven, M (Seven, Mehmet); Ulucan, H (Ulucan, Hakan); Yuksel, A (Yuksel, Adnan); Ozen, M (Ozen, Mustafa)
GENE, 563:2, 215-218, DOI: 10.1016/j.gene.2015.03.060, 1 2015
Özet için tıklayınızUsher syndrome is a clinically and genetically heterogeneous autosomal recessive inherited disorder accompanied by hearing loss and retinitis pigmentosa (RP). Since the associated genes are various and quite large, we utilized whole-exome sequencing (WES) as a diagnostic tool to identify the molecular basis of Usher syndrome. DNA from a 12-year-old male diagnosed with Usher syndrome was analyzed by WES. Mutations detected were confirmed by Sanger sequencing. The pathogenicity of these mutations was determined by in silico analysis.
A maternally inherited deleterious frameshift mutation, c.14439_14454del in exon 66 and a paternally inherited non-sense c.10830G>A stop-gain SNV in exon 55 of USH2A were found as two novel compound heterozygous mutations. Both of these mutations disrupt the C terminal of USH2A protein.
As a result, WES revealed two novel compound heterozygous mutations in a Turkish USH2A patient. This approach gave us an opportunity to have an appropriate diagnosis and provide genetic counseling to the family within a reasonable rime. (C) 2015 Elsevier B.V. All rights reserved.
An ethnobotanical survey of medicinal plants in western part of central Taurus Mountains: Aladaglar (Nigde - Turkey)
Ozdemir, E (Ozdemir, Ebru); Alpinar, K (Alpinar, Kerim)
JOURNAL OF ETHNOPHARMACOLOGY, 166, 53-65, DOI: 10.1016/j.jep.2015.02.052, 26 2015
Özet için tıklayınızEthnopharmacological relevance: With this study, we aimed to document traditional uses of medicinal plants in the western part of Aladaglar/Nigde.
Materials and methods: This study was conducted between 2003 and 2005. The research area was in the western part of the Aladaglar mountains. The settlements in Aladaglar (5 towns and 10 villages) were visited during the field work The plants collected by the help of medicinal plant users. The plants were identified and voucher specimens prepared. These voucher specimens were kept at the Herbarium of Istanbul University Faculty of Pharmacy (ISTE). We collected the information by means of semi-structured interviews with 170 informants (90 men and 80 women). In addition, the relative importance value of the species was determined and the informant consensus factor (FIC) was calculated for the medicinal plants researched in the study.
Results: According to the results of the identification, among 126 plants were used by the inhabitants and 110 species belonging to 40 families were used for medicinal purposes. Most of the medicinal plants used in Aladaglar/Nigde belong to the families Lamiaceae (25 species), Asteraceae (16 species), Apiaceae (7 species), Fabaceae (6 species) and Brassicaceae (5 species). The most commonly used plant species were Hypericum perforatum Thymus sipyleus var. sipyleus, Rosa canina, Urtica dioica, Malva neglecta, Thymus leucotrichus, Salix alba, Mentha longifolia, Berberis crataegina, Juniperus oxycedrus, Viscum album subsp. abietis, Allium rotundum and Taraxacum stevenii. The most common preparations were infusion and decoction. The traditional medicinal plants have been mostly used for the treatment of cardiovascular diseases (86%), hemorrhoids (79%), urinary diseases (69%), diabetes (68%) and respiratory diseases (61%).
Conclusion: The use of traditional medicine was still widespread among the inhabitants of Aladaglar mountains/Nigde region. Due to the lack of medical facilities in the villages of Aladaglar mountains, local people prefer herbal treatment rather than medical treatment. This study identified not only the wild plants collected for medical purposes by local people of Aladaglar/Nigde, but also the uses and local names of these plants. This paper helps to preserve valuable information that may otherwise be lost to future generations. (c) 2015 Elsevier Ireland Ltd. All rights reserved.
Differential Expression of Hypertension-Associated MicroRNAs in the Plasma of Patients With White Coat Hypertension
Cengiz, M (Cengiz, Mahir); Karatas, OF (Karatas, Omer Faruk); Koparir, E (Koparir, Erkan); Yavuzer, S (Yavuzer, Serap); Ali, C (Ali, Chayar); Yavuzer, H (Yavuzer, Hakan); Kirat, E (Kirat, Emre); Karter, Y (Karter, Yesari); Ozen, M (Ozen, Mustafa)
MEDICINE, 94:13 Article Number: e693, DOI: 10.1097/MD.0000000000000693, 2015
Özet için tıklayınızWhite coat hypertension (WCH) is a high cardiovascular risk condition, and a fundamental understanding of the cause and pathophysiology of the disorder is still lacking. Recent studies demonstrated that microRNAs (miRNAs) are involved in hypertension; however, the roles of miRNAs in WCH are not known.
The expressions of selected 10 miRNAs were investigated independently in plasma samples from 30 hypertension (HT) patients, 30 WCH patients, and 30 normotensive (NT) subjects.
MiR-21, miR-122, miR-637, and let-7e expression levels were significantly upregulated in the HT group compared with the NT groups (P = 0.017, P = 0.022, P = 0.048, and P = 0.013, respectively). MiR-122 and miR-637 expressions were also significantly upregulated in the WCH group compared with the NT group (P = 0.048 and P = 0.039, respectively). MiR-296-5p expression level was significantly down-regulated in HT patients and upregulated in the WCH patients compared with the NT group (P = 0.049 and P = 0.039, respectively).
Additionally, the ambulatory 24-hour and daytime systolic and diastolic blood pressures were negatively correlated with miR-2965p. MiR-296 and miR-637 had area under the curve (AUC) values of 0.778 and 0.774, respectively, which demonstrates their sufficiency to distinguish WCH from NT individuals. MiR-296 and miR-637 had AUC values of 0.868 and 0.680, respectively, which shows their potential to distinguish WCH from HT individuals.
We report for the first time a plasma miRNA profile for WCH patients and demonstrate a novel link between miRNA and WCH. These findings may reveal crucial insights into the development of WCH.
Effects of Nd:YAG Laser Irradiation for Minimizing Edema and Pain After Sinus Lift Surgery: Randomized Controlled Clinical Trial
Ozturan, S (Ozturan, Seda); Sirali, A (Sirali, Ali); Sur, H (Sur, Haydar)
PHOTOMEDICINE AND LASER SURGERY, 33:4, 193-199, DOI: 10.1089/pho.2014.3823, 1 2015
Özet için tıklayınızObjective: The aim of the present study was to evaluate the effectiveness of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser used for low-level laser therapy (LLLT) on pain, oral health-related quality of life (OHRQoL), and swelling after sinus lift procedure. Materials and methods: Ten healthy patients, requiring bilateral direct sinus lift surgery, were included in this prospective, randomized study. The treatment sides were randomly assigned to LLLT or control groups. The experimental side received 8 J/cm(2) of energy density, a wavelength of 1064 nm, for 60 sec, and 0.5 W laser immediately after surgery. Swelling, pain perception, and OHRQoL were evaluated at different time intervals. The data were analyzed by the one way ANOVA. Results: Evaluations of postoperative pain, OHRQoL, and swelling were made. We observed that the swelling and the OHRQoL in the Nd:YAG group were significantly improved when compared with the control group on the 3rd day after surgery (p<0.05). The pain levels after surgery was almost the same for both groups, without statistically significant differences (p>0.05). There were no statistically significant differences between groups with regard to any of the parameters evaluated on the 7th day. Conclusions: The results demonstrate that the 1064 nm Nd:YAG laser was effective in improving OHRQoL and reducing swelling after sinus lift surgery. Within the limitations of this study, we concluded that the 1064 nm Nd:YAG laser has beneficial effects on swelling and OHRQoL, making it suitable for LLLT.
Identification of microRNAs differentially expressed in prostatic secretions of patients with prostate cancer
Guzel, E (Guzel, Esra); Karatas, OF (Karatas, Omer F.); Semercioz, A (Semercioz, Atilla); Ekici, S (Ekici, Sinan); Aykan, S (Aykan, Serdar); Yentur, S (Yentur, Serhat); Creighton, CJ (Creighton, Chad J.); Ittmann, M (Ittmann, Michael); Ozen, M (Ozen, Mustafa)
INTERNATIONAL JOURNAL OF CANCER, 136:4, 875-879, DOI: 10.1002/ijc.29054, 15 2015
Özet için tıklayınızProstate cancer (PCa) is one of the leading causes of cancer deaths in men. Since there are limited treatment options available for the advanced tumors, there is an urgent need for novel diagnostic tools for PCa. Prostate secretion samples (PSS) from 23 PCa and 25 benign prostate hyperplasia (BPH) patients were obtained from Urology Department of Bagcilar Educational and Research Hospital (Istanbul). MicroRNA (miRNA) profiling of eight PSS (four from BPH, four from PCa patients) was performed using microarray. Four of significantly deregulated miRNAs were further confirmed using quantitative reverse-transcription PCR (qRT-PCR). Statistical analysis was performed using Student's t-test. ROC curves were plotted with SPSS-15.0. In this study, we aimed to identify a miRNA expression signature that could be used to distinguish PCa from BPH. MiRNA profiling of four PCa and four BPH patients with microarray revealed that miR-361-3p, miR-133b and miR-221 were significantly downregulated and miR-203 was upregulated in PSS of PCa patients. Further qRT-PCR analysis confirmed the altered expressions of these four miRNAs in PSS of 23 PCa and 25 BPH patients. Four miRNAs, together and individually have much power (AUC; 0.950) than PSA has (AUC; 0.463) to discriminate PCa from BPH patients. We have shown for the first time in the literature the presence of miRNAs in the PSS. We suggest PSS as a powerful non-invasive Dergi for evaluation of prognosis in PCa, since prostate massages can be easily applied during routine examination. Our results showed that certain differentially expressed miRNAs in PSS could be used as diagnostics markers.
What's new? The association of microRNAs (miRNAs) with cancer initiation, progression, and metastasis has fueled increasing interest in their potential as diagnostic and therapeutic markers. The current study aimed to identify an miRNA expression signature that could be used to distinguish prostate cancer from benign prostatic hyperplasia by using prostate secretions obtained from patients. MiRNAs were found to be present in the prostate secretion samples, with altered expression detected for four miRNAs in particular. The findings suggest that certain miRNAs may be powerful tools for aiding prostate cancer diagnosis.
Assessment of the soft tSayı Cilt Notric changes following acellular dermal matrix grafts with cone beam computerized tomography
Ozturan, S (Ozturan, Seda); Oztunc, H (Oztunc, Haluk); Evlice, BK (Evlice, Burcu Keles)
QUINTESSENCE INTERNATIONAL, 46:2, 171-178, DOI: 10.3290/j.qi.a32826, 2015
Özet için tıklayınızObjective: The aim of this study was to use cone beam computerized tomography (CBCT), which consistently allowed the determination of the dimensions of the soft tSayı, to evaluate acellular dermal matrix grafts (ADMG) used to increase gingival soft tSayı thickness (GSTT). Method and Materials: In total, 90 gingival defects were included in the study. Surgical procedures were performed to augment the gingival tSayı with ADMG. While GSTT measurements were assessed at baseline and 6, 12, and 18 months post-surgery, ADMG measurements were performed with CBCT at 6, 12, and 18 months post-sur-gery. Results: All parameters showed statistically significant differences between time intervals. In the ADMG-treated sites, the baseline GSTT was 1.00 +/- 0.37 mm and the final thickness was 1.66 +/- 0.34 mm. The 6-month measurement of ADMG was 0.79 +/- 0.08 mm, and the final measurement was 0.11 +/- 0.09 mm. Conclusion: High-quality images of the GSTT and ADMG can be consistently obtained with CBCT. The present findings indicate that soft tSayı augmentation can be achieved in gingival defects with the use of ADMG.
Chronic pain, depression symptoms and daily living independency level among geriatrics in nursing home
Tarakci, E (Tarakci, Ela); Zenginler, Y (Zenginler, Yonca); Kaya Mutlu, E (Kaya Mutlu, Ebru)
AGRI-THE JOURNAL OF THE TURKISH SOCIETY OF ALGOLOGY, 27:1, 35-41, DOI: 10.5505/agri.2015.14238 Basım Tarihi: JAN 2015
Özet için tıklayınızObjectives: The aim of this study was to investigate pain, depression and independence in activities of daily living in geriatric residents of nursing homes, as well as to evaluate the relationship between these parameters.
Methods: 186 nursing home residents, aged 65 to 90 years, were enrolled in the study. Their socio-demographic features, depression levels, pain levels and independence in activities of daily living were evaluated using a socio-demographic assessment form, the Geriatric Depression Scale (GDS), the Visual Analogue Scale (VAS) and the Nottingham Extended Activities of Daily Living Index (NEADL), respectively. For statistical analyses, participants were divided into groups with and without chronic pain.
Results: The main finding of this study was that 55.9% of the participants reported chronic pain, and these participants had significantly higher GDS (p=0.001) and lower NEADL scores (p=0.01) than those who reported no chronic pain. We found a significant correlation between VAS and GDS (r=0.47, p=0.001), VAS and NEADL (r=-0.30, p=0.001), and GDS and NEADL scores (r=-0.50, p=0.001). Female gender (p=0.001), number of children (p=0.005), number of chronic diseases (p=0.009), and GDS score (p=0.001) were found to affect chronic pain in multivariate model.
Conclusion: Investigation of pain, presence of depression, and independence in activities of daily living is important in determining the necessary measures to be adopted for promoting the health and well-being of the geriatric population.
Comparison of risk factors for cardiovascular disease in hemodialysis and peritoneal dialysis patients
Harmankaya, O (Harmankaya, Ozlem); Akalin, N (Akalin, Nilgul); Akay, H (Akay, Hatice); Okuturlar, Y (Okuturlar, Yildiz); Erturk, K (Erturk, Kayhan); Kaptanogullari, H (Kaptanogullari, Hakan); Kocoglu, H (Kocoglu, Hakan)
CLINICS, 70:9, 601-605, DOI: 10.6061/clinics/2015(09)01 Basım Tarihi: 2015
Özet için tıklayınızOBJECTIVE: In this study, we aimed to compare the cardiovascular risk factors that might be associated with inflammation, atherosclerosis and metabolic syndrome between hemodialysis and peritoneal dialysis patients.
METHODS: Fifty hemodialysis and 50 peritoneal dialysis patients who had been receiving dialysis therapy for at least one year were included in the study. Venous blood samples were taken after 12 hours of fasting, and serum glucose, triglyceride, low-density lipoprotein (LDL)-cholesterol, high-density lipoprotein (HDL)-cholesterol, C-reactive protein, fibrinogen and homocysteine levels were measured. The presence of atherosclerotic plaques in the carotid artery was evaluated by carotid Doppler ultrasound. These data were analyzed by Student's t test, the chi-square test and the Mann-Whitney U test, as appropriate.
RESULTS: No difference was found between the hemodialysis (n=50) and peritoneal dialysis (n=50) patient groups regarding mean age, gender distribution, body mass index or dialysis duration (p=0.269, 0.683, 0.426, and 0.052, respectively). LDL-cholesterol, fibrinogen and homocysteine levels were significantly higher in peritoneal dialysis patients (p=0.006, 0.001, and 0.002, respectively). In patients with diabetes mellitus (n=17) who were undergoing renal replacement therapy, LDL-cholesterol and fibrinogen levels were significantly higher than in patients without diabetes mellitus who were undergoing renal replacement therapy (p=0.001 and 0.004, respectively).
CONCLUSION: In our study, cardiovascular risk factors especially LDL-cholesterol) were more frequent in peritoneal dialysis patients than in hemodialysis patients.
Overexpression of miR-145-5p Inhibits Proliferation of Prostate Cancer Cells and Reduces SOX2 Expression
Ozen, M (Ozen, Mustafa); Karatas, OF (Karatas, Omer Faruk); Gulluoglu, S (Gulluoglu, Sukru); Bayrak, OF (Bayrak, Omer Faruk); Sevli, S (Sevli, Serhat); Guzel, E (Guzel, Esra); Ekici, ID (Ekici, Isin D.); Caskurlu, T (Caskurlu, Turhan); Solak, M (Solak, Mustafa); Creighton, CJ (Creighton, Chad J.); Ittmann, M (Ittmann, Michael)
CANCER INVESTIGATION, 33:6, 251-258, DOI: 10.3109/07357907.2015.1025407 Basım Tarihi: 2015
Özet için tıklayınızWe aimed to perform functional analysis of miR-145-5p in prostate cancer (PCa) cells and to identify targets of miR-145-5p for understanding its role in PCa pathogenesis. PC3, DU145, LNCaP PCa, and PNT1a nontumorigenic prostate cell lines were utilized for functional analysis of miR-145-5p. Its overexpression caused inhibition of proliferation through apoptosis and reduced migration in PCa cells. SOX2 expression was significantly decreased in both mRNA and protein level in miR-145-5p-overexpressed PCa cells. We proposed that miR-145-5p, being an important regulator of SOX2, carries a crucial role in PCa tumorigenesis.
Designing a gold nanoparticle-based nanocarrier for microRNA transfection into the prostate and breast cancer cells
Ekin, A (Ekin, Asli); Karatas, OF (Karatas, Omer Faruk); Culha, M (Culha, Mustafa); Ozen, M (Ozen, Mustafa)
JOURNAL OF GENE MEDICINE, 16:11-12, 331-335, DOI: 10.1002/jgm.2810, 2014
Özet için tıklayınızBackgroundCancer is one of the most common causes of human deaths worldwide. Nanotechnology has the potential to facilitate the detection, diagnosis, and treatment of cancer cases. Successful delivery of nucleic acids into cancer cells with the use of nanoparticles would be a significant improvement for medical and cellular biology. The use of nanoparticle-based vehicles in clinical treatment is considerably important for treating genetic disorders. Gold nanoparticles (AuNPs) have been suggested as therapeutic delivery tools for cancer. Because microRNAs (miRNAs), which induce post-transcriptional gene silencing, are deregulated in cancer cells, they are also considered as strong candidates for cancer therapy applications. In prostate and breast cancer, miR-145, a well-known tumor suppressor miRNA, is strongly downregulated in tumor tSayıs compared to their corresponding normal tSayıs.
MethodsIn the present study, we aimed to use engineered AuNPs as nanocarrier platforms to deliver miRNAs to prostate/breast cancer cells. 13-nm AuNPs were modified with thiolated RNAs and then the miR-145 was hybridized to the RNAs that were chemically attached to the AuNPs.
ResultsThe results obtained in the present study demonstrate the efficient delivery of miR-145 to prostate/breast cancer cells. We also show that delivery was more efficient when the AuNP-RNA-miRNA carrier complex was formed at an elevated temperature of 72 degrees C.
ConclusionsIn conclusion, we show that AuNPs help the effective in vitro delivery of miR-145 into cancer cells. Copyright (c) 2014 John Wiley & Sons, Ltd.
Differential Expression of Stem Cell Markers and ABCG2 in Recurrent Prostate Cancer
Guzel, E (Guzel, Esra); Karatas, OF (Karatas, Omer F.); Duz, MB (Duz, Mehmet B.); Solak, M (Solak, Mustafa); Ittmann, M (Ittmann, Michael); Ozen, M (Ozen, Mustafa)
PROSTATE, 74:15, 1498-1505, DOI: 10.1002/pros.22867, 2014
Özet için tıklayınızBACKGROUND. Prostate cancer (PCa) is the second most common tumor type related to mortality in males in the developed countries. Studies have demonstrated that therapeutic tools mostly ineffective to give positive outcome especially for PCa. Cancer stem cells are composed of a small cell population, which are supposed to have roles in tumorigenesis, metastasis, and tumor recurrence after chemo-radiotherapy. The aim of this research is to investigate expressions of stem cell markers in recurrent PCa and non-recurrent PCa tumors as well as in adjacent normal prostate tSayıs.
METHODS. We compared the expression of important sternness regulators like SOX2, OCT4, KLF4, and ABCG2 in recurrent, non-recurrent PCa and adjacent normal tSayı samples using quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS. Our results demonstrated that SOX2 and OCT4 are strongly overexpressed in PCa samples. Recurrent PCa samples are markedly positive for stem cell markers SOX2, OCT4, and KLF4. Furthermore, non-recurrent PCa samples presented low levels of ABCG2, a multidrug resistance protein, compared to both normal and recurrent samples, which might be associated with chemo-sensitivity.
CONCLUSIONS. Enhanced expression of ABCG2 and stem cell markers including SOX2, OCT4, and KLF4 in the recurrent PCa tSayıs postulates the suggestion that enrichment for cells with stem cell characteristics in these tSayıs might be playing a critical role for chemoresistance and recurrence of cancer. (C) 2014 Wiley Periodicals, Inc.
Ex vivo protective effects of nicotinamide and 3-aminobenzamide on rat synaptosomes treated with A beta(1-42)
Bayrakdar, ET (Bayrakdar, Ezgi Turunc); Armagan, G (Armagan, Guliz); Uyanikgil, Y (Uyanikgil, Yigit); Kanit, L (Kanit, Lutfiye); Koylu, E (Koylu, Ersin); Yalcin, A (Yalcin, Ayfer)
CELL BIOCHEMISTRY AND FUNCTION, 32:7, 557-564, DOI: 10.1002/cbf.3049, 2014
Özet için tıklayınızAlzheimer's disease (AD) is the most common form of dementia and is characterized by the presence of senile plaques and neurofibrillary tangles, along with synaptic loss. The underlying mechanisms of AD are not clarified yet, but oxidative stress and mitochondrial dysfunction are important factors. Overactivation of poly(adenosine diphosphate ribose) polymerase-1 (PARP-1) enzyme has been known to cause neuroinflammation and cell death in neurodegenerative processes. The aim of the present study was to investigate the protective effects of the PARP-1 inhibitors, 3-aminobenzamide (3-AB) and nicotinamide (NA), against amyloid peptide (1-42) (A(1-42))-induced oxidative damage and mitochondrial reduction capacity on isolated synaptosomes. Rats were injected intraperitoneally with 3-AB (30-100mgkg(-1)), NA (100-500mgkg(-1)) or with saline for 7days. Synaptosomes were incubated with 10-30M A(1-42) or saline for 6h at 37 degrees C. Ex vivo A(1-42) treatment significantly induced oxidative stress and mitochondrial dysfunction in synaptosomes of the saline group, while synaptosomes of 3-AB and NA groups showed significant decreases in lipid peroxidation, reactive oxygen species production and protein oxidation. Moreover, both NA and 3-AB were able to improve the mitochondrial reduction capacity against A(1-42). These data suggest that NA and 3-AB may have protective effects in neurodegenerative processes because of the reduced levels of oxidative stress and the improvement of mitochondrial function. Copyright (c) 2014 John Wiley & Sons, Ltd.
Poikiloderma with Neutropenia: Genotype-Ethnic Origin Correlation, Expanding Phenotype and Literature Review
Koparir, A (Koparir, Asuman); Gezdirici, A (Gezdirici, Alper); Koparir, E (Koparir, Erkan); Ulucan, H (Ulucan, Hakan); Yilmaz, M (Yilmaz, Mehmet); Erdemir, A (Erdemir, Asli); Yuksel, A (Yuksel, Adnan); Ozen, M (Ozen, Mustafa)
AMERICAN JOURNAL OF MEDICAL GENETICS PART A, 164:10, 2535-2540, DOI: 10.1002/ajmg.a.36683, 2014
Özet için tıklayınızPoikiloderma with neutropenia (PN), is a rare genodermatosis associated with patognomic features of poikiloderma and permanent neutropenia. Three common recurrent mutations of related gene, USB1, were considered to be associated with three different ethnic origins. The most common recurrent mutation, c.531delA, has been detected in seven Caucasian patients in the literature. In this paper, we present review of all patients from the literature and report two additional patients of Turkish ancestry with the diagnosis of PN. The diagnosis of these two PN patients were made clinically and confirmed by molecular analysis which detected the most common recurrent mutation, c.531delA. Genotype-ethnic origin correlation hypothesis, therefore, has been strengthened with this result. Short stature in PN, is a common finding, which until now has never been treated with growth hormone (GH). One of our patients is the first patient with attempted treatment of short stature via GH administration. Finally, both of our patients had high-pitched voice and vocal cord nodules which might be considered as additional clinical findings not associated with PN before. (C) 2014 Wiley Periodicals, Inc.
Case Series of Laser-Assisted Treatment of Excessive Gingival Display: An Alternative Treatment
Ozturan, S (Ozturan, Seda); Ay, E (Ay, Elif); Sagir, S (Sagir, Serkan)
PHOTOMEDICINE AND LASER SURGERY, 32:9, 517-523, DOI: 10.1089/pho.2014.3737, 2014
Özet için tıklayınızObjective: The objective of this study was to investigate the stable and significant outcomes of laser-assisted lip repositioning technique for the treatment of excessive gingival display. Background data: "Gummy smile'' is a major concern for a large number of patients visiting the dentist. The hyperactivity of the upper lip is one of the main causes of a gummy smile, and several techniques have been proposed for its treatment. Methods: Ten patients with excessive gingival display were treated with laser-assisted lip repositioning technique. The technique is composed of removing a strip of mucosa by laser incisions from the maxillary buccal vestibule, and coronal repositioning of the new mucosal margin. The clinical dimensions of gingival display were measured from the left, right posterior, and anterior sides at baseline, 6 and 12 months postoperatively. Subjects completed surveys to evaluate satisfaction with outcomes. Clinical and patient-centered parameters [visual analog scale for pain and aesthetics (VAS)] were measured at 7 days and again at 12 months after the surgery. Results: The baseline anterior gingival display of 4.3 +/- 1.8 mm significantly decreased to 1.1 +/- 1.0 mm at 6 months (p < 0.0001) and was maintained at 12 months (1.2 +/- 1.5 mm). The baseline left, right gingival display (4.9 +/- 2.2 mm; 5.0 +/- 1.4 mm) significantly decreased (1.2 +/- 1.4 mm; 1.3 +/- 1.1 mm) at 12 months (p < 0.0001). Patients were satisfied with their smile after surgery and would likely choose to undergo the procedure again. Conclusions: The treatment of gummy smile with laser-assisted lip repositioning successfully reduced the preoperative gingival display without postoperative complications, in all cases.
miR-1 and miR-133b Are Differentially Expressed in Patients with Recurrent Prostate Cancer
Karatas, OF (Karatas, Omer Faruk); Guzel, E (Guzel, Esra); Suer, I (Suer, Ilknur); Ekici, ID (Ekici, Isin D.); Caskurlu, T (Caskurlu, Turhan); Creighton, CJ (Creighton, Chad J.); Ittmann, M (Ittmann, Michael); Ozen, M (Ozen, Mustafa)
PLOS ONE, 9:6 Article Number: e98675, DOI: 10.1371/journal.pone.0098675, 26 2014
Özet için tıklayınızProstate cancer (PCa) is currently the most frequently diagnosed malignancy in the western countries. It is more prevalent in older men with 75% of the incident cases above 65 years old. After radical prostatectomy, approximately 30% of men develop clinical recurrence with elevated serum prostate-specific antigen levels. Therefore, it is important to unravel the molecular mechanisms underlying PCa progression to develop novel diagnostic/therapeutic approaches. In this study, it is aimed to compare the microRNA (miRNA) profile of recurrent and non-recurrent prostate tumor tSayıs to explore the possible involvement of miRNAs in PCa progression. Total RNA from 41 recurrent and 41 non-recurrent PCa tSayı samples were used to investigate the miRNA signature in PCa specimens. First of all, 20 recurrent and 20 non-recurrent PCa samples were profiled using miRNA microarray chips. Of the differentially expressed miRNAs, miR-1, miR-133b and miR-145* were selected for further validation with qRT-PCR in a different set of 21 recurrent and 21 non-recurrent PCa samples. Data were statistically analyzed using two-sided Student's t-test, Pearson Correlation test, Receiver operating characteristic analysis. Our results demonstrated that miR-1 and mir-133b have been significantly downregulated in recurrent PCa specimens in comparison to non-recurrent PCa samples and have sufficient power to distinguish recurrent specimens from non-recurrent ones on their own. Here, we report that the relative expression of miR-1 and mir-133b have been significantly reduced in recurrent PCa specimens in comparison to non-recurrent PCa samples, which can serve as novel biomarkers for prediction of PCa progression.